Microbial oil produced from biodiesel by-products could enhance overall production
Glycerol and rapeseed meal, two major by-products of biodiesel production, have been tested for possible use as low-cost raw materials for the production of microbial bio-oil using the oleaginous yeast Rhodosporidium toruloides. Using fed-batch fermentation with crude glycerol and a novel nitrogen r...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/96617 http://hdl.handle.net/10220/11798 |
| _version_ | 1826119481620955136 |
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| author | Uçkun Kiran, Esra Trzcinski, Antoine Webb, Colin |
| author2 | Advanced Environmental Biotechnology Centre (AEBC) |
| author_facet | Advanced Environmental Biotechnology Centre (AEBC) Uçkun Kiran, Esra Trzcinski, Antoine Webb, Colin |
| author_sort | Uçkun Kiran, Esra |
| collection | NTU |
| description | Glycerol and rapeseed meal, two major by-products of biodiesel production, have been tested for possible use as low-cost raw materials for the production of microbial bio-oil using the oleaginous yeast Rhodosporidium toruloides. Using fed-batch fermentation with crude glycerol and a novel nitrogen rich nutrient source derived from rapeseed meal as feed, it was shown that 13 g/L lipids could be produced, compared with 9.4 g/L when crude glycerol was used with yeast extract. When 100 g/L pure glycerol was used, the final lipid concentration was 19.7 g/L with the novel biomedium compared to 16.2 g/L for yeast extract. The novel biomedium also resulted in higher lipid yields (0.19 g lipid/g glycerol consumed compared to 0.12 g/L) suggesting it provides a better carbon to nitrogen balance for accumulating lipids. FAMEs produced from the microbial lipids indicated a high degree of unsaturation confirming that the fatty acids produced from the novel biomedium have potential for biodiesel production. |
| first_indexed | 2024-10-01T05:00:43Z |
| format | Journal Article |
| id | ntu-10356/96617 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:00:43Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/966172020-03-07T12:48:42Z Microbial oil produced from biodiesel by-products could enhance overall production Uçkun Kiran, Esra Trzcinski, Antoine Webb, Colin Advanced Environmental Biotechnology Centre (AEBC) DRNTU::Engineering::Chemical engineering::Biotechnology Glycerol and rapeseed meal, two major by-products of biodiesel production, have been tested for possible use as low-cost raw materials for the production of microbial bio-oil using the oleaginous yeast Rhodosporidium toruloides. Using fed-batch fermentation with crude glycerol and a novel nitrogen rich nutrient source derived from rapeseed meal as feed, it was shown that 13 g/L lipids could be produced, compared with 9.4 g/L when crude glycerol was used with yeast extract. When 100 g/L pure glycerol was used, the final lipid concentration was 19.7 g/L with the novel biomedium compared to 16.2 g/L for yeast extract. The novel biomedium also resulted in higher lipid yields (0.19 g lipid/g glycerol consumed compared to 0.12 g/L) suggesting it provides a better carbon to nitrogen balance for accumulating lipids. FAMEs produced from the microbial lipids indicated a high degree of unsaturation confirming that the fatty acids produced from the novel biomedium have potential for biodiesel production. 2013-07-17T08:48:58Z 2019-12-06T19:33:03Z 2013-07-17T08:48:58Z 2019-12-06T19:33:03Z 2012 2012 Journal Article Uçkun Kiran, E., Trzcinski, A., & Webb, C. (2013). Microbial oil produced from biodiesel by-products could enhance overall production. Bioresource Technology, 129, 650-654. 0960-8524 https://hdl.handle.net/10356/96617 http://hdl.handle.net/10220/11798 10.1016/j.biortech.2012.11.152 171274 en Bioresource technology © 2012 Elsevier Ltd. |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Biotechnology Uçkun Kiran, Esra Trzcinski, Antoine Webb, Colin Microbial oil produced from biodiesel by-products could enhance overall production |
| title | Microbial oil produced from biodiesel by-products could enhance overall production |
| title_full | Microbial oil produced from biodiesel by-products could enhance overall production |
| title_fullStr | Microbial oil produced from biodiesel by-products could enhance overall production |
| title_full_unstemmed | Microbial oil produced from biodiesel by-products could enhance overall production |
| title_short | Microbial oil produced from biodiesel by-products could enhance overall production |
| title_sort | microbial oil produced from biodiesel by products could enhance overall production |
| topic | DRNTU::Engineering::Chemical engineering::Biotechnology |
| url | https://hdl.handle.net/10356/96617 http://hdl.handle.net/10220/11798 |
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