Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane
We previously reported that mitotic endoplasmic reticulum (ER) membrane cisternae or sheets directly assemble mammalian nuclear envelope (NE) at the end of mitosis. In this study, we investigated the dynamics of the high curvature regions of partially assembled nuclear envelope membrane using reti...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2013
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| Online Access: | https://hdl.handle.net/10356/96720 http://hdl.handle.net/10220/13053 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291306/ |
| _version_ | 1811694266766852096 |
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| author | Lu, Lei Kirchhausen, Tomas. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Lu, Lei Kirchhausen, Tomas. |
| author_sort | Lu, Lei |
| collection | NTU |
| description | We previously reported that mitotic endoplasmic reticulum (ER) membrane cisternae or sheets directly assemble
mammalian nuclear envelope (NE) at the end of mitosis. In this study, we investigated the dynamics of the high curvature
regions of partially assembled nuclear envelope membrane using reticulon4a as a probe. We found that, after sorting out
reticulon4a from the nascent NE membrane sheets, reticulon4a is specifically localized to the leading edges. Our 3D time
lapse images suggested that ER tubules could be incompetent in assembling the NE membrane. Our findings suggest a
possible role of reticulons at the leading edges during the NE re-assembly and provide further evidences that the mitotic
assembly of NE is by ER cisternae rather than tubules. |
| first_indexed | 2024-10-01T07:04:51Z |
| format | Journal Article |
| id | ntu-10356/96720 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:04:51Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/967202023-02-28T16:59:13Z Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane Lu, Lei Kirchhausen, Tomas. School of Biological Sciences DRNTU::Science::Biological sciences We previously reported that mitotic endoplasmic reticulum (ER) membrane cisternae or sheets directly assemble mammalian nuclear envelope (NE) at the end of mitosis. In this study, we investigated the dynamics of the high curvature regions of partially assembled nuclear envelope membrane using reticulon4a as a probe. We found that, after sorting out reticulon4a from the nascent NE membrane sheets, reticulon4a is specifically localized to the leading edges. Our 3D time lapse images suggested that ER tubules could be incompetent in assembling the NE membrane. Our findings suggest a possible role of reticulons at the leading edges during the NE re-assembly and provide further evidences that the mitotic assembly of NE is by ER cisternae rather than tubules. Accepted version 2013-08-06T04:45:51Z 2019-12-06T19:34:15Z 2013-08-06T04:45:51Z 2019-12-06T19:34:15Z 2011 2011 Journal Article https://hdl.handle.net/10356/96720 http://hdl.handle.net/10220/13053 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291306/ en Communicative & integrative biology © 2012 Landes Bioscience. This is the author created version of a work that has been peer reviewed and accepted for publication by Communicative & integrative biology, Landes Bioscience. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291306/]. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Lu, Lei Kirchhausen, Tomas. Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane |
| title | Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane |
| title_full | Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane |
| title_fullStr | Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane |
| title_full_unstemmed | Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane |
| title_short | Visualizing the high curvature regions of post-mitotic nascent nuclear envelope membrane |
| title_sort | visualizing the high curvature regions of post mitotic nascent nuclear envelope membrane |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/96720 http://hdl.handle.net/10220/13053 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3291306/ |
| work_keys_str_mv | AT lulei visualizingthehighcurvatureregionsofpostmitoticnascentnuclearenvelopemembrane AT kirchhausentomas visualizingthehighcurvatureregionsofpostmitoticnascentnuclearenvelopemembrane |