Active control of SPR by thermoresponsive hydrogels for biosensor applications
The use of thermoresponsive poly(N-isopropylacrylamide)-based hydrogel (pNIPAAm) for rapid tuning of surface plasmon resonance (SPR) is reported. This approach is implemented by using an SPR layer architecture with an embedded indium tin oxide microheater and pNIPAAm film on its top. It takes advant...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/96738 http://hdl.handle.net/10220/18043 |
| _version_ | 1826111841418346496 |
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| author | Toma, Mana Jonas, Ulrich Mateescu, Anca Knoll, Wolfgang Dostálek, Jakub |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Toma, Mana Jonas, Ulrich Mateescu, Anca Knoll, Wolfgang Dostálek, Jakub |
| author_sort | Toma, Mana |
| collection | NTU |
| description | The use of thermoresponsive poly(N-isopropylacrylamide)-based hydrogel (pNIPAAm) for rapid tuning of surface plasmon resonance (SPR) is reported. This approach is implemented by using an SPR layer architecture with an embedded indium tin oxide microheater and pNIPAAm film on its top. It takes advantage of rapid thermally induced swelling and collapse of pNIPAAm that is accompanied by large refractive index changes and leads to high thermo-optical coefficient of dn/dT = 2 × 10–2 RIU/K. We show that this material is excellently suited for efficient control of refractive index-sensitive SPR and that it can serve simultaneously as a 3D binding matrix in biosensor applications (if modified with biomolecular recognition elements for a specific capture of target analyte). We demonstrate that this approach enables modulating of the output signal in surface plasmon-enhanced fluorescence spectroscopy biosensors and holds potential for simple time-multiplexing of sensing channels for parallelized readout of fluorescence assays. |
| first_indexed | 2024-10-01T02:57:27Z |
| format | Journal Article |
| id | ntu-10356/96738 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:57:27Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/967382022-02-16T16:28:59Z Active control of SPR by thermoresponsive hydrogels for biosensor applications Toma, Mana Jonas, Ulrich Mateescu, Anca Knoll, Wolfgang Dostálek, Jakub School of Materials Science & Engineering DRNTU::Engineering::Materials::Biomaterials The use of thermoresponsive poly(N-isopropylacrylamide)-based hydrogel (pNIPAAm) for rapid tuning of surface plasmon resonance (SPR) is reported. This approach is implemented by using an SPR layer architecture with an embedded indium tin oxide microheater and pNIPAAm film on its top. It takes advantage of rapid thermally induced swelling and collapse of pNIPAAm that is accompanied by large refractive index changes and leads to high thermo-optical coefficient of dn/dT = 2 × 10–2 RIU/K. We show that this material is excellently suited for efficient control of refractive index-sensitive SPR and that it can serve simultaneously as a 3D binding matrix in biosensor applications (if modified with biomolecular recognition elements for a specific capture of target analyte). We demonstrate that this approach enables modulating of the output signal in surface plasmon-enhanced fluorescence spectroscopy biosensors and holds potential for simple time-multiplexing of sensing channels for parallelized readout of fluorescence assays. 2013-12-05T01:14:49Z 2019-12-06T19:34:25Z 2013-12-05T01:14:49Z 2019-12-06T19:34:25Z 2013 2013 Journal Article Toma, M., Jonas, U., Mateescu, A., Knoll, W., & Dostálek, J. (2013). Active control of SPR by thermoresponsive hydrogels for biosensor applications. The journal of physical chemistry C, 117(22), 11705-11712. https://hdl.handle.net/10356/96738 http://hdl.handle.net/10220/18043 10.1021/jp400255u 23762499 en The journal of physical chemistry C |
| spellingShingle | DRNTU::Engineering::Materials::Biomaterials Toma, Mana Jonas, Ulrich Mateescu, Anca Knoll, Wolfgang Dostálek, Jakub Active control of SPR by thermoresponsive hydrogels for biosensor applications |
| title | Active control of SPR by thermoresponsive hydrogels for biosensor applications |
| title_full | Active control of SPR by thermoresponsive hydrogels for biosensor applications |
| title_fullStr | Active control of SPR by thermoresponsive hydrogels for biosensor applications |
| title_full_unstemmed | Active control of SPR by thermoresponsive hydrogels for biosensor applications |
| title_short | Active control of SPR by thermoresponsive hydrogels for biosensor applications |
| title_sort | active control of spr by thermoresponsive hydrogels for biosensor applications |
| topic | DRNTU::Engineering::Materials::Biomaterials |
| url | https://hdl.handle.net/10356/96738 http://hdl.handle.net/10220/18043 |
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