Cytotoxicity evaluation of oxidized single-walled carbon nanotubes and graphene oxide on human Hepatoma HepG2 cells : an iTRAQ-coupled 2D LC-MS/MS proteome analysis
Because of their attractive chemical and physical properties, graphitic nanomaterials and their derivatives have gained tremendous interest for applications in electronics, materials, and biomedical areas. However, few detailed studies have been performed to evaluate the potential cytotoxicity of th...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2013
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| Online Access: | https://hdl.handle.net/10356/97115 http://hdl.handle.net/10220/11827 |
| _version_ | 1811689981395075072 |
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| author | Yuan, Jifeng Gao, Hongcai Sui, Jianjun Duan, Hongwei Chen, William Wei Ning Ching, Chi Bun |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Yuan, Jifeng Gao, Hongcai Sui, Jianjun Duan, Hongwei Chen, William Wei Ning Ching, Chi Bun |
| author_sort | Yuan, Jifeng |
| collection | NTU |
| description | Because of their attractive chemical and physical properties, graphitic nanomaterials and their derivatives have gained tremendous interest for applications in electronics, materials, and biomedical areas. However, few detailed studies have been performed to evaluate the potential cytotoxicity of these nanomaterials on living systems at the molecular level. In the present study, our group exploited the isobaric tagged relative and absolute quantification (iTRAQ)–coupled two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) approach with the purpose of characterizing the cellular functions in response to these nanomaterials at the proteome level. Specifically, the human hepatoma HepG2 cells were selected as the in vitro model to study the potential cytotoxicity of oxidized single-walled carbon nanotubes (SWCNTs) and graphene oxide (GO) on the vital organ of liver. Overall, 30 differentially expressed proteins involved in metabolic pathway, redox regulation, cytoskeleton formation, and cell growth were identified. Based on the protein profile, we found oxidized SWCNTs induced oxidative stress and interfered with intracellular metabolic routes, protein synthesis, and cytoskeletal systems. Further functional assays confirmed that oxidized SWCNTs triggered elevated level of reactive oxygen species (ROS), perturbed the cell cycle, and resulted in a significant increase in the proportion of apoptotic cells. However, only moderate variation of protein levels for the cells treated with GO was observed and functional assays further confirmed that GO was less cytotoxic in comparison to oxidized SWCNTs. These finding suggested that GO was more biocompatible and could be a promising candidate for bio-related applications. |
| first_indexed | 2024-10-01T05:56:44Z |
| format | Journal Article |
| id | ntu-10356/97115 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:56:44Z |
| publishDate | 2013 |
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| spelling | ntu-10356/971152020-03-07T11:35:36Z Cytotoxicity evaluation of oxidized single-walled carbon nanotubes and graphene oxide on human Hepatoma HepG2 cells : an iTRAQ-coupled 2D LC-MS/MS proteome analysis Yuan, Jifeng Gao, Hongcai Sui, Jianjun Duan, Hongwei Chen, William Wei Ning Ching, Chi Bun School of Chemical and Biomedical Engineering Because of their attractive chemical and physical properties, graphitic nanomaterials and their derivatives have gained tremendous interest for applications in electronics, materials, and biomedical areas. However, few detailed studies have been performed to evaluate the potential cytotoxicity of these nanomaterials on living systems at the molecular level. In the present study, our group exploited the isobaric tagged relative and absolute quantification (iTRAQ)–coupled two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) approach with the purpose of characterizing the cellular functions in response to these nanomaterials at the proteome level. Specifically, the human hepatoma HepG2 cells were selected as the in vitro model to study the potential cytotoxicity of oxidized single-walled carbon nanotubes (SWCNTs) and graphene oxide (GO) on the vital organ of liver. Overall, 30 differentially expressed proteins involved in metabolic pathway, redox regulation, cytoskeleton formation, and cell growth were identified. Based on the protein profile, we found oxidized SWCNTs induced oxidative stress and interfered with intracellular metabolic routes, protein synthesis, and cytoskeletal systems. Further functional assays confirmed that oxidized SWCNTs triggered elevated level of reactive oxygen species (ROS), perturbed the cell cycle, and resulted in a significant increase in the proportion of apoptotic cells. However, only moderate variation of protein levels for the cells treated with GO was observed and functional assays further confirmed that GO was less cytotoxic in comparison to oxidized SWCNTs. These finding suggested that GO was more biocompatible and could be a promising candidate for bio-related applications. 2013-07-18T02:31:41Z 2019-12-06T19:39:06Z 2013-07-18T02:31:41Z 2019-12-06T19:39:06Z 2011 2011 Journal Article Yuan, J., Gao, H., Sui, J., Duan, H., Chen, W. N. W., & Ching, C. B. (2012). Cytotoxicity evaluation of oxidized single-walled carbon nanotubes and graphene oxide on human Hepatoma HepG2 cells : an iTRAQ-coupled 2D LC-MS/MS proteome analysis. Toxicological sciences, 126(1), 149-161. https://hdl.handle.net/10356/97115 http://hdl.handle.net/10220/11827 10.1093/toxsci/kfr332 en Toxicological sciences © 2011 The Authors. |
| spellingShingle | Yuan, Jifeng Gao, Hongcai Sui, Jianjun Duan, Hongwei Chen, William Wei Ning Ching, Chi Bun Cytotoxicity evaluation of oxidized single-walled carbon nanotubes and graphene oxide on human Hepatoma HepG2 cells : an iTRAQ-coupled 2D LC-MS/MS proteome analysis |
| title | Cytotoxicity evaluation of oxidized single-walled carbon nanotubes and graphene oxide on human Hepatoma HepG2 cells : an iTRAQ-coupled 2D LC-MS/MS proteome analysis |
| title_full | Cytotoxicity evaluation of oxidized single-walled carbon nanotubes and graphene oxide on human Hepatoma HepG2 cells : an iTRAQ-coupled 2D LC-MS/MS proteome analysis |
| title_fullStr | Cytotoxicity evaluation of oxidized single-walled carbon nanotubes and graphene oxide on human Hepatoma HepG2 cells : an iTRAQ-coupled 2D LC-MS/MS proteome analysis |
| title_full_unstemmed | Cytotoxicity evaluation of oxidized single-walled carbon nanotubes and graphene oxide on human Hepatoma HepG2 cells : an iTRAQ-coupled 2D LC-MS/MS proteome analysis |
| title_short | Cytotoxicity evaluation of oxidized single-walled carbon nanotubes and graphene oxide on human Hepatoma HepG2 cells : an iTRAQ-coupled 2D LC-MS/MS proteome analysis |
| title_sort | cytotoxicity evaluation of oxidized single walled carbon nanotubes and graphene oxide on human hepatoma hepg2 cells an itraq coupled 2d lc ms ms proteome analysis |
| url | https://hdl.handle.net/10356/97115 http://hdl.handle.net/10220/11827 |
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