N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks
A novel N- to C-terminus sequential chemical ligation approach has been developed for protein synthesis. Key to this strategy is the relative stability of the N,N-bis(2-mercaptoethyl)amide (BMEA) to the conventional conditions of native chemical ligation. We have also found a new thiol additive for...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/97425 http://hdl.handle.net/10220/10577 |
| _version_ | 1826128744349171712 |
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| author | Liu, Chuan-Fa Yang, Renliang. Hou, Wen. Zhang, Xiaohong. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Liu, Chuan-Fa Yang, Renliang. Hou, Wen. Zhang, Xiaohong. |
| author_sort | Liu, Chuan-Fa |
| collection | NTU |
| description | A novel N- to C-terminus sequential chemical ligation approach has been developed for protein synthesis. Key to this strategy is the relative stability of the N,N-bis(2-mercaptoethyl)amide (BMEA) to the conventional conditions of native chemical ligation. We have also found a new thiol additive for the BMEA-mediated ligation reaction. The usefulness of this approach was demonstrated in the syntheses of a medium-sized peptide and ubiquitin. |
| first_indexed | 2024-10-01T07:29:35Z |
| format | Journal Article |
| id | ntu-10356/97425 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:29:35Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/974252020-03-07T12:18:19Z N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks Liu, Chuan-Fa Yang, Renliang. Hou, Wen. Zhang, Xiaohong. School of Biological Sciences DRNTU::Science::Biological sciences A novel N- to C-terminus sequential chemical ligation approach has been developed for protein synthesis. Key to this strategy is the relative stability of the N,N-bis(2-mercaptoethyl)amide (BMEA) to the conventional conditions of native chemical ligation. We have also found a new thiol additive for the BMEA-mediated ligation reaction. The usefulness of this approach was demonstrated in the syntheses of a medium-sized peptide and ubiquitin. 2013-06-25T02:18:11Z 2019-12-06T19:42:39Z 2013-06-25T02:18:11Z 2019-12-06T19:42:39Z 2011 2011 Journal Article Yang, R., Hou, W., Zhang, X., & Liu, C. F. (2012). N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks. Organic letters, 14(1), 374-377. 1523-7060 https://hdl.handle.net/10356/97425 http://hdl.handle.net/10220/10577 10.1021/ol2031284 en Organic letters © 2011 American Chemical Society. |
| spellingShingle | DRNTU::Science::Biological sciences Liu, Chuan-Fa Yang, Renliang. Hou, Wen. Zhang, Xiaohong. N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks |
| title | N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks |
| title_full | N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks |
| title_fullStr | N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks |
| title_full_unstemmed | N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks |
| title_short | N-to-C sequential ligation using peptidyl N,N-Bis(2-mercaptoethyl)amide building blocks |
| title_sort | n to c sequential ligation using peptidyl n n bis 2 mercaptoethyl amide building blocks |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/97425 http://hdl.handle.net/10220/10577 |
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