Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics
Real-time monitoring of cell apoptosis could provide valuable insights into early detection of therapy efficiency and evaluation of disease progression. In this work, we designed and synthesized a new live-cell-permeable, fluorescent light-up probe for real-time cell apoptosis imaging. The probe is...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/97650 http://hdl.handle.net/10220/11235 |
| _version_ | 1811688644542464000 |
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| author | Kwok, Ryan T. K. Shi, Haibin Liu, Jianzhao Xing, Bengang Tang, Ben Zhong Liu, Bin |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Kwok, Ryan T. K. Shi, Haibin Liu, Jianzhao Xing, Bengang Tang, Ben Zhong Liu, Bin |
| author_sort | Kwok, Ryan T. K. |
| collection | NTU |
| description | Real-time monitoring of cell apoptosis could provide valuable insights into early detection of therapy efficiency and evaluation of disease progression. In this work, we designed and synthesized a new live-cell-permeable, fluorescent light-up probe for real-time cell apoptosis imaging. The probe is comprised of a hydrophilic caspase-specific Asp-Glu-Val-Asp (DEVD) peptide and a hydrophobic tetraphenylethene (TPE) unit, a typical fluorogen with aggregation-induced emission characteristics. In aqueous solution, the probe is almost nonfluorescent but displays significant fluorescence enhancement in response to caspase-3/-7, which are activated in the apoptotic process and able to cleave the DEVD moieties. This fluorescence “turn-on” response is ascribed to aggregation of cleaved hydrophobic TPE residues, which restricts the intramolecular rotations of TPE phenyl rings and populates the radiative decay channels. The light-up nature of the probe allows real-time monitoring of caspase-3/-7 activities both in solutions and in living cells with a high signal-to-noise ratio. The probe provides a new opportunity to screen enzyme inhibitors and evaluate the apoptosis-associated drug efficacy. |
| first_indexed | 2024-10-01T05:35:29Z |
| format | Journal Article |
| id | ntu-10356/97650 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:35:29Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/976502020-03-07T12:31:27Z Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics Kwok, Ryan T. K. Shi, Haibin Liu, Jianzhao Xing, Bengang Tang, Ben Zhong Liu, Bin School of Physical and Mathematical Sciences Real-time monitoring of cell apoptosis could provide valuable insights into early detection of therapy efficiency and evaluation of disease progression. In this work, we designed and synthesized a new live-cell-permeable, fluorescent light-up probe for real-time cell apoptosis imaging. The probe is comprised of a hydrophilic caspase-specific Asp-Glu-Val-Asp (DEVD) peptide and a hydrophobic tetraphenylethene (TPE) unit, a typical fluorogen with aggregation-induced emission characteristics. In aqueous solution, the probe is almost nonfluorescent but displays significant fluorescence enhancement in response to caspase-3/-7, which are activated in the apoptotic process and able to cleave the DEVD moieties. This fluorescence “turn-on” response is ascribed to aggregation of cleaved hydrophobic TPE residues, which restricts the intramolecular rotations of TPE phenyl rings and populates the radiative decay channels. The light-up nature of the probe allows real-time monitoring of caspase-3/-7 activities both in solutions and in living cells with a high signal-to-noise ratio. The probe provides a new opportunity to screen enzyme inhibitors and evaluate the apoptosis-associated drug efficacy. 2013-07-11T08:14:06Z 2019-12-06T19:44:56Z 2013-07-11T08:14:06Z 2019-12-06T19:44:56Z 2012 2012 Journal Article Shi, H., Kwok, R. T. K., Liu, J., Xing, B., Tang, B. Z.,& Liu, B. (2012). Real-Time Monitoring of Cell Apoptosis and Drug Screening Using Fluorescent Light-Up Probe with Aggregation-Induced Emission Characteristics. Journal of the American Chemical Society, 134(43), 17972-17981. https://hdl.handle.net/10356/97650 http://hdl.handle.net/10220/11235 10.1021/ja3064588 en Journal of the American chemical society © 2012 American Chemical Society. |
| spellingShingle | Kwok, Ryan T. K. Shi, Haibin Liu, Jianzhao Xing, Bengang Tang, Ben Zhong Liu, Bin Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics |
| title | Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics |
| title_full | Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics |
| title_fullStr | Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics |
| title_full_unstemmed | Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics |
| title_short | Real-time monitoring of cell apoptosis and drug screening using fluorescent light-up probe with aggregation-induced emission characteristics |
| title_sort | real time monitoring of cell apoptosis and drug screening using fluorescent light up probe with aggregation induced emission characteristics |
| url | https://hdl.handle.net/10356/97650 http://hdl.handle.net/10220/11235 |
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