Gene silencing of human neuronal cells for drug addiction therapy using anisotropic nanocrystals
Theranostic platform integrating diagnostic imaging and therapeutic function into a single system has become a new direction of nanoparticle research. In the process of treatment, therapeutic efficacy is monitored. The use of theranostic nanoparticle can add an additional "layer" to keep t...
| Main Authors: | , , , , , , , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/97675 http://hdl.handle.net/10220/11858 |
| _version_ | 1811692958234181632 |
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| author | Mahajan, Supriya D. Reynolds, Jessica L. Schwartz, Stanley A. Prasad, Paras N. Law, Wing-Cheung Kopwitthaya, Atcha Liu, Maixian Liu, Xin Chen, Guanying Erogbogbo, Folarin Vathy, Lisa Aalinkeel, Ravikumar Yong, Ken-Tye |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Mahajan, Supriya D. Reynolds, Jessica L. Schwartz, Stanley A. Prasad, Paras N. Law, Wing-Cheung Kopwitthaya, Atcha Liu, Maixian Liu, Xin Chen, Guanying Erogbogbo, Folarin Vathy, Lisa Aalinkeel, Ravikumar Yong, Ken-Tye |
| author_sort | Mahajan, Supriya D. |
| collection | NTU |
| description | Theranostic platform integrating diagnostic imaging and therapeutic function into a single system has become a new direction of nanoparticle research. In the process of treatment, therapeutic efficacy is monitored. The use of theranostic nanoparticle can add an additional "layer" to keep track on the therapeutic agent such as the pharmacokinetics and biodistribution. In this report, we have developed quantum rod (QR) based formulations for the delivery of small interfering RNAs (siRNAs) to human neuronal cells. PEGlyated QRs with different surface functional groups (amine and maleimide) were designed for selectively down-regulating the dopaminergic signaling pathway which is associated with the drug abuse behavior. We have demonstrated that the DARPP-32 siRNAs were successfully delivered to dopaminergic neuronal (DAN) cells which led to drastic knockdown of specific gene expression by both the electrostatic and covalent bond conjugation regimes. The PEGlyated surface offered high biocompatibilities and negligible cytotoxicities to the QR formulations that may facilitate the in vivo applications of these nanoparticles. |
| first_indexed | 2024-10-01T06:44:03Z |
| format | Journal Article |
| id | ntu-10356/97675 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:44:03Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/976752022-02-16T16:31:20Z Gene silencing of human neuronal cells for drug addiction therapy using anisotropic nanocrystals Mahajan, Supriya D. Reynolds, Jessica L. Schwartz, Stanley A. Prasad, Paras N. Law, Wing-Cheung Kopwitthaya, Atcha Liu, Maixian Liu, Xin Chen, Guanying Erogbogbo, Folarin Vathy, Lisa Aalinkeel, Ravikumar Yong, Ken-Tye School of Electrical and Electronic Engineering DRNTU::Engineering::Electrical and electronic engineering Theranostic platform integrating diagnostic imaging and therapeutic function into a single system has become a new direction of nanoparticle research. In the process of treatment, therapeutic efficacy is monitored. The use of theranostic nanoparticle can add an additional "layer" to keep track on the therapeutic agent such as the pharmacokinetics and biodistribution. In this report, we have developed quantum rod (QR) based formulations for the delivery of small interfering RNAs (siRNAs) to human neuronal cells. PEGlyated QRs with different surface functional groups (amine and maleimide) were designed for selectively down-regulating the dopaminergic signaling pathway which is associated with the drug abuse behavior. We have demonstrated that the DARPP-32 siRNAs were successfully delivered to dopaminergic neuronal (DAN) cells which led to drastic knockdown of specific gene expression by both the electrostatic and covalent bond conjugation regimes. The PEGlyated surface offered high biocompatibilities and negligible cytotoxicities to the QR formulations that may facilitate the in vivo applications of these nanoparticles. Published Version 2013-07-18T04:13:08Z 2019-12-06T19:45:17Z 2013-07-18T04:13:08Z 2019-12-06T19:45:17Z 2012 2012 Journal Article Law, W.-C., Mahajan, S. D., Kopwitthaya, A., Reynolds, J. L., Liu, M., Liu, X., et al. (2012). Gene Silencing of Human Neuronal Cells for Drug Addiction Therapy using Anisotropic Nanocrystals. Theranostics, 2(7), 695-704. 1838-7640 https://hdl.handle.net/10356/97675 http://hdl.handle.net/10220/11858 10.7150/thno.3459 22896771 en Theranostics © 2012 Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/ licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. application/pdf |
| spellingShingle | DRNTU::Engineering::Electrical and electronic engineering Mahajan, Supriya D. Reynolds, Jessica L. Schwartz, Stanley A. Prasad, Paras N. Law, Wing-Cheung Kopwitthaya, Atcha Liu, Maixian Liu, Xin Chen, Guanying Erogbogbo, Folarin Vathy, Lisa Aalinkeel, Ravikumar Yong, Ken-Tye Gene silencing of human neuronal cells for drug addiction therapy using anisotropic nanocrystals |
| title | Gene silencing of human neuronal cells for drug addiction therapy using anisotropic nanocrystals |
| title_full | Gene silencing of human neuronal cells for drug addiction therapy using anisotropic nanocrystals |
| title_fullStr | Gene silencing of human neuronal cells for drug addiction therapy using anisotropic nanocrystals |
| title_full_unstemmed | Gene silencing of human neuronal cells for drug addiction therapy using anisotropic nanocrystals |
| title_short | Gene silencing of human neuronal cells for drug addiction therapy using anisotropic nanocrystals |
| title_sort | gene silencing of human neuronal cells for drug addiction therapy using anisotropic nanocrystals |
| topic | DRNTU::Engineering::Electrical and electronic engineering |
| url | https://hdl.handle.net/10356/97675 http://hdl.handle.net/10220/11858 |
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