Engineering of erythrocyte-based drug carriers : control of protein release and bioactivity
This work reports the fabrication of layer-by-layer (LbL) polyelectrolyte coated erythrocyte carriers that provide a simple means for controlling the burst and subsequent release of lysozyme. Erythrocytes were loaded with RITC-lysozyme as model compound via the hypotonic dialysis method. An encapsul...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/97719 http://hdl.handle.net/10220/18105 |
| _version_ | 1826119699747831808 |
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| author | Luo, Rongcong Shaillender, Mutukumaraswamy Venkatraman, Subbu S. Neu, Björn |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Luo, Rongcong Shaillender, Mutukumaraswamy Venkatraman, Subbu S. Neu, Björn |
| author_sort | Luo, Rongcong |
| collection | NTU |
| description | This work reports the fabrication of layer-by-layer (LbL) polyelectrolyte coated erythrocyte carriers that provide a simple means for controlling the burst and subsequent release of lysozyme. Erythrocytes were loaded with RITC-lysozyme as model compound via the hypotonic dialysis method. An encapsulation efficiency of 41.6% and a loading amount of 12.7 pg/cell was achieved. It is demonstrated that these carriers maintain their shape and integrity similar to natural erythrocytes after the encapsulation procedures, and achieve a uniform distribution of the encapsulated lysozyme. The erythrocyte carriers were fixed with glutaraldehyde and then successfully coated with biocompatible polyelectrolytes, poly-l-lysine hydrobromide and dextran sulfate, using the LbL method. It is demonstrated that the release profile of the encapsulated macromolecule can be regulated by adjusting the number of polyelectrolyte layers. Furthermore by adjusting the concentrations of the cross linking agent the activity of the encapsulated lysozyme can be well preserved. These core–shell microcapsules, consisting of erythrocytes loaded with bioactive substances and coated with a polyelectrolyte multilayer shell, hold promise for a new type of biocompatible and biodegradable drug delivery system. |
| first_indexed | 2024-10-01T05:04:22Z |
| format | Journal Article |
| id | ntu-10356/97719 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:04:22Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/977192020-06-01T10:01:58Z Engineering of erythrocyte-based drug carriers : control of protein release and bioactivity Luo, Rongcong Shaillender, Mutukumaraswamy Venkatraman, Subbu S. Neu, Björn School of Chemical and Biomedical Engineering School of Materials Science & Engineering DRNTU::Engineering::Materials::Biomaterials This work reports the fabrication of layer-by-layer (LbL) polyelectrolyte coated erythrocyte carriers that provide a simple means for controlling the burst and subsequent release of lysozyme. Erythrocytes were loaded with RITC-lysozyme as model compound via the hypotonic dialysis method. An encapsulation efficiency of 41.6% and a loading amount of 12.7 pg/cell was achieved. It is demonstrated that these carriers maintain their shape and integrity similar to natural erythrocytes after the encapsulation procedures, and achieve a uniform distribution of the encapsulated lysozyme. The erythrocyte carriers were fixed with glutaraldehyde and then successfully coated with biocompatible polyelectrolytes, poly-l-lysine hydrobromide and dextran sulfate, using the LbL method. It is demonstrated that the release profile of the encapsulated macromolecule can be regulated by adjusting the number of polyelectrolyte layers. Furthermore by adjusting the concentrations of the cross linking agent the activity of the encapsulated lysozyme can be well preserved. These core–shell microcapsules, consisting of erythrocytes loaded with bioactive substances and coated with a polyelectrolyte multilayer shell, hold promise for a new type of biocompatible and biodegradable drug delivery system. 2013-12-05T06:14:20Z 2019-12-06T19:45:50Z 2013-12-05T06:14:20Z 2019-12-06T19:45:50Z 2012 2012 Journal Article Luo, R., Shaillender, M., Venkatraman, S. S., & Neu, B. (2012). Engineering of erythrocyte-based drug carriers : control of protein release and bioactivity. Journal of materials science : materials in medicine, 23(1), 63-71. https://hdl.handle.net/10356/97719 http://hdl.handle.net/10220/18105 10.1007/s10856-011-4485-2 en Journal of materials science : Materials in medicine |
| spellingShingle | DRNTU::Engineering::Materials::Biomaterials Luo, Rongcong Shaillender, Mutukumaraswamy Venkatraman, Subbu S. Neu, Björn Engineering of erythrocyte-based drug carriers : control of protein release and bioactivity |
| title | Engineering of erythrocyte-based drug carriers : control of protein release and bioactivity |
| title_full | Engineering of erythrocyte-based drug carriers : control of protein release and bioactivity |
| title_fullStr | Engineering of erythrocyte-based drug carriers : control of protein release and bioactivity |
| title_full_unstemmed | Engineering of erythrocyte-based drug carriers : control of protein release and bioactivity |
| title_short | Engineering of erythrocyte-based drug carriers : control of protein release and bioactivity |
| title_sort | engineering of erythrocyte based drug carriers control of protein release and bioactivity |
| topic | DRNTU::Engineering::Materials::Biomaterials |
| url | https://hdl.handle.net/10356/97719 http://hdl.handle.net/10220/18105 |
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