Applications of saturation transfer difference NMR in biological systems
The method of saturation transfer difference (STD) nuclear magnetic resonance (NMR) is an indispensable NMR tool in drug discovery. It identifies binding epitope(s) at the atomic resolution of small molecule ligands (e.g. organic drugs, peptides and oligosaccharides), while interacting with their re...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/97921 http://hdl.handle.net/10220/13241 |
| _version_ | 1811697408405405696 |
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| author | Bhunia, Anirban Bhattacharjya, Surajit Chatterjee, Subhrangsu |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Bhunia, Anirban Bhattacharjya, Surajit Chatterjee, Subhrangsu |
| author_sort | Bhunia, Anirban |
| collection | NTU |
| description | The method of saturation transfer difference (STD) nuclear magnetic resonance (NMR) is an indispensable NMR tool in drug discovery. It identifies binding epitope(s) at the atomic resolution of small molecule ligands (e.g. organic drugs, peptides and oligosaccharides), while interacting with their receptors, such as proteins and/or nucleic acids. The method is widely used to screen active drug molecules, simultaneously ranking them in a qualitative way. STD NMR is highly successful for a variety of high molecular weight systems, such as whole viruses, platelets, intact cells, lipopolysaccharide micelles, membrane proteins, recombinant proteins and dispersion pigments. Modifications of STD pulse programs using 13C and 15N nuclei are now used to overcome the signal overlapping that occurs with more complex structures. |
| first_indexed | 2024-10-01T07:54:47Z |
| format | Journal Article |
| id | ntu-10356/97921 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:54:47Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/979212020-03-07T12:18:18Z Applications of saturation transfer difference NMR in biological systems Bhunia, Anirban Bhattacharjya, Surajit Chatterjee, Subhrangsu School of Biological Sciences The method of saturation transfer difference (STD) nuclear magnetic resonance (NMR) is an indispensable NMR tool in drug discovery. It identifies binding epitope(s) at the atomic resolution of small molecule ligands (e.g. organic drugs, peptides and oligosaccharides), while interacting with their receptors, such as proteins and/or nucleic acids. The method is widely used to screen active drug molecules, simultaneously ranking them in a qualitative way. STD NMR is highly successful for a variety of high molecular weight systems, such as whole viruses, platelets, intact cells, lipopolysaccharide micelles, membrane proteins, recombinant proteins and dispersion pigments. Modifications of STD pulse programs using 13C and 15N nuclei are now used to overcome the signal overlapping that occurs with more complex structures. 2013-08-27T06:02:34Z 2019-12-06T19:48:22Z 2013-08-27T06:02:34Z 2019-12-06T19:48:22Z 2012 2012 Journal Article Bhunia, A., Bhattacharjya, S., & Chatterjee, S. (2012). Applications of saturation transfer difference NMR in biological systems. Drug Discovery Today, 17(9-10), 505-513. https://hdl.handle.net/10356/97921 http://hdl.handle.net/10220/13241 10.1016/j.drudis.2011.12.016 en Drug discovery today |
| spellingShingle | Bhunia, Anirban Bhattacharjya, Surajit Chatterjee, Subhrangsu Applications of saturation transfer difference NMR in biological systems |
| title | Applications of saturation transfer difference NMR in biological systems |
| title_full | Applications of saturation transfer difference NMR in biological systems |
| title_fullStr | Applications of saturation transfer difference NMR in biological systems |
| title_full_unstemmed | Applications of saturation transfer difference NMR in biological systems |
| title_short | Applications of saturation transfer difference NMR in biological systems |
| title_sort | applications of saturation transfer difference nmr in biological systems |
| url | https://hdl.handle.net/10356/97921 http://hdl.handle.net/10220/13241 |
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