Oxidation of DNA bases influenced by the presence of other bases
Electrochemical detection of DNA is a highly important topic. Here we show that the electrochemical responses of one DNA base (guanine, adenine, cytosine or thymine), in terms of oxidation potential, current intensity, peak width and resolution can be highly influenced by the presence of other DNA...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/98173 http://hdl.handle.net/10220/13258 |
| _version_ | 1826125213339746304 |
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| author | Shuhua Goh, Madeline Pumera, Martin |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Shuhua Goh, Madeline Pumera, Martin |
| author_sort | Shuhua Goh, Madeline |
| collection | NTU |
| description | Electrochemical detection of DNA is a highly important topic. Here we show that the electrochemical responses of
one DNA base (guanine, adenine, cytosine or thymine), in terms of oxidation potential, current intensity, peak
width and resolution can be highly influenced by the presence of other DNA bases at electrochemically reduced
graphene oxide (ER-GO) as well as standard glassy carbon electrode. We have observed that the effects were more
significant for adenine base on ER-GO and cytosine base on glassy carbon (GC) electrode. Differences in responses
were generally low in a mixture of four different DNA bases but interestingly, deviations become significantly
larger when only one or two other bases were present. Our findings are of paramount importance for future developments
in DNA detection and analysis since individual DNA bases are not present in isolation in nature or in typical
biosensing systems. |
| first_indexed | 2024-10-01T06:33:02Z |
| format | Journal Article |
| id | ntu-10356/98173 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:33:02Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/981732020-03-07T12:34:44Z Oxidation of DNA bases influenced by the presence of other bases Shuhua Goh, Madeline Pumera, Martin School of Physical and Mathematical Sciences Electrochemical detection of DNA is a highly important topic. Here we show that the electrochemical responses of one DNA base (guanine, adenine, cytosine or thymine), in terms of oxidation potential, current intensity, peak width and resolution can be highly influenced by the presence of other DNA bases at electrochemically reduced graphene oxide (ER-GO) as well as standard glassy carbon electrode. We have observed that the effects were more significant for adenine base on ER-GO and cytosine base on glassy carbon (GC) electrode. Differences in responses were generally low in a mixture of four different DNA bases but interestingly, deviations become significantly larger when only one or two other bases were present. Our findings are of paramount importance for future developments in DNA detection and analysis since individual DNA bases are not present in isolation in nature or in typical biosensing systems. 2013-08-29T07:23:51Z 2019-12-06T19:51:46Z 2013-08-29T07:23:51Z 2019-12-06T19:51:46Z 2012 2012 Journal Article Shuhua Goh, M.,& Pumera, M. (2012). Oxidation of DNA Bases Influenced by the Presence of Other Bases. Electroanalysis, 24(5), 1147-1152. 1040-0397 https://hdl.handle.net/10356/98173 http://hdl.handle.net/10220/13258 10.1002/elan.201200036 en Electroanalysis |
| spellingShingle | Shuhua Goh, Madeline Pumera, Martin Oxidation of DNA bases influenced by the presence of other bases |
| title | Oxidation of DNA bases influenced by the presence of other bases |
| title_full | Oxidation of DNA bases influenced by the presence of other bases |
| title_fullStr | Oxidation of DNA bases influenced by the presence of other bases |
| title_full_unstemmed | Oxidation of DNA bases influenced by the presence of other bases |
| title_short | Oxidation of DNA bases influenced by the presence of other bases |
| title_sort | oxidation of dna bases influenced by the presence of other bases |
| url | https://hdl.handle.net/10356/98173 http://hdl.handle.net/10220/13258 |
| work_keys_str_mv | AT shuhuagohmadeline oxidationofdnabasesinfluencedbythepresenceofotherbases AT pumeramartin oxidationofdnabasesinfluencedbythepresenceofotherbases |