Imaging of surface O2 dynamics in corals with magnetic micro optode particles
We present a new method for quantifying spatio-temporal O2 distribution and dynamics at biologically active surfaces with a complex surface topography. Magnetized O2 optode microparticles (~80–100 μm) containing the NIR-emitting luminophore platinum (II) meso-tetra(4-fluorophenyl) tetrabenzoporphyri...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/98271 http://hdl.handle.net/10220/17322 |
| _version_ | 1811682943942262784 |
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| author | Borisov, Sergey M. Klimant, Ingo. Kühl, Michael. Fabricius-Dyg, J. Mistlberger, G. Staal, M. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Borisov, Sergey M. Klimant, Ingo. Kühl, Michael. Fabricius-Dyg, J. Mistlberger, G. Staal, M. |
| author_sort | Borisov, Sergey M. |
| collection | NTU |
| description | We present a new method for quantifying spatio-temporal O2 distribution and dynamics at biologically active surfaces with a complex surface topography. Magnetized O2 optode microparticles (~80–100 μm) containing the NIR-emitting luminophore platinum (II) meso-tetra(4-fluorophenyl) tetrabenzoporphyrin (PtTPTBPF; ex. max. 615 nm; em. max. 780 nm) were distributed across the surface tissue of the scleractinian coral Caulastrea furcata and were held in place with a strong magnet. The O2-dependent luminescence of the particles was mapped with a lifetime imaging system enabling measurements of the lateral surface heterogeneity of the O2 microenvironment across coral polyps exposed to flow. Mapping steady-state O2 concentrations under constant light and O2 dynamics during experimental light–dark shifts enabled us to identify zones of different photosynthetic activities within a single coral polyp linked to the distribution of coral host pigments. Measurements under increasing irradiance showed typical saturation curves of O2 concentration and estimates of gross photosynthesis that could be spatially resolved at ~100 μm pixel resolution. The new method for O2 imaging with magnetized optode particles has much potential to be used in studies of the surface microenvironment of other aquatic systems such as sediments, biofilms, plant, and animal tissue. |
| first_indexed | 2024-10-01T04:04:53Z |
| format | Journal Article |
| id | ntu-10356/98271 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T04:04:53Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/982712020-03-07T12:18:19Z Imaging of surface O2 dynamics in corals with magnetic micro optode particles Borisov, Sergey M. Klimant, Ingo. Kühl, Michael. Fabricius-Dyg, J. Mistlberger, G. Staal, M. School of Biological Sciences DRNTU::Science::Biological sciences We present a new method for quantifying spatio-temporal O2 distribution and dynamics at biologically active surfaces with a complex surface topography. Magnetized O2 optode microparticles (~80–100 μm) containing the NIR-emitting luminophore platinum (II) meso-tetra(4-fluorophenyl) tetrabenzoporphyrin (PtTPTBPF; ex. max. 615 nm; em. max. 780 nm) were distributed across the surface tissue of the scleractinian coral Caulastrea furcata and were held in place with a strong magnet. The O2-dependent luminescence of the particles was mapped with a lifetime imaging system enabling measurements of the lateral surface heterogeneity of the O2 microenvironment across coral polyps exposed to flow. Mapping steady-state O2 concentrations under constant light and O2 dynamics during experimental light–dark shifts enabled us to identify zones of different photosynthetic activities within a single coral polyp linked to the distribution of coral host pigments. Measurements under increasing irradiance showed typical saturation curves of O2 concentration and estimates of gross photosynthesis that could be spatially resolved at ~100 μm pixel resolution. The new method for O2 imaging with magnetized optode particles has much potential to be used in studies of the surface microenvironment of other aquatic systems such as sediments, biofilms, plant, and animal tissue. 2013-11-05T08:22:48Z 2019-12-06T19:53:03Z 2013-11-05T08:22:48Z 2019-12-06T19:53:03Z 2012 2012 Journal Article Fabricius-Dyg, J., Mistlberger, G., Staal, M., Borisov, S. M., Klimant, I., & Kühl, M. (2012). Imaging of surface O2 dynamics in corals with magnetic micro optode particles. Marine biology, 159(7), 1621-1631. https://hdl.handle.net/10356/98271 http://hdl.handle.net/10220/17322 10.1007/s00227-012-1920-y en Marine biology |
| spellingShingle | DRNTU::Science::Biological sciences Borisov, Sergey M. Klimant, Ingo. Kühl, Michael. Fabricius-Dyg, J. Mistlberger, G. Staal, M. Imaging of surface O2 dynamics in corals with magnetic micro optode particles |
| title | Imaging of surface O2 dynamics in corals with magnetic micro optode particles |
| title_full | Imaging of surface O2 dynamics in corals with magnetic micro optode particles |
| title_fullStr | Imaging of surface O2 dynamics in corals with magnetic micro optode particles |
| title_full_unstemmed | Imaging of surface O2 dynamics in corals with magnetic micro optode particles |
| title_short | Imaging of surface O2 dynamics in corals with magnetic micro optode particles |
| title_sort | imaging of surface o2 dynamics in corals with magnetic micro optode particles |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/98271 http://hdl.handle.net/10220/17322 |
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