Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus
A sensitive membrane-based electrochemical nanobiosensor is developed for the detection of dengue type 2 virus (DENV-2) using nanoporous alumina-modified platinum electrode. Its sensing mechanism relies on the monitoring of electrode's Faradaic current response toward redox probe, ferrocenemeth...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/98333 http://hdl.handle.net/10220/12430 |
| _version_ | 1826125494145253376 |
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| author | Cheng, Ming Soon Ho, Jia Shin Tan, Cheong Huat Wong, Jeslyn Pei Sze Ng, Lee Ching Toh, Chee-Seng |
| author2 | School of Physical and Mathematical Sciences |
| author_facet | School of Physical and Mathematical Sciences Cheng, Ming Soon Ho, Jia Shin Tan, Cheong Huat Wong, Jeslyn Pei Sze Ng, Lee Ching Toh, Chee-Seng |
| author_sort | Cheng, Ming Soon |
| collection | NTU |
| description | A sensitive membrane-based electrochemical nanobiosensor is developed for the detection of dengue type 2 virus (DENV-2) using nanoporous alumina-modified platinum electrode. Its sensing mechanism relies on the monitoring of electrode's Faradaic current response toward redox probe, ferrocenemethanol, which is sensitive toward the formation of immune complexes within the alumina nanochannels. Anti-DENV-2 monoclonal antibody (clone 3H5, isotype IgG) is used as the biorecognition element in this work. The stepwise additions of antibody, bovine serum albumin (BSA) and DENV-2 are characterized by differential pulse voltammetry (DPV). A low detection limit of 1 pfu mL−1 with linear range from 1 to 103 pfu mL−1 (R2 = 0.98) can be achieved by the nanobiosensor. The nanobiosensor is selective toward DENV-2 with insignificant cross reaction with non-specific viruses, Chikungunya virus, West Nile virus and dengue type 3 virus (DENV-3). Relative standard deviation (RSD) for triplicate analysis of 5.9% indicates an acceptable level of reproducibility. The first direct quantitation of DENV-2 concentration in whole mosquito vector is demonstrated using this electrochemical nanobiosensor. |
| first_indexed | 2024-10-01T06:37:33Z |
| format | Journal Article |
| id | ntu-10356/98333 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:37:33Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/983332020-03-07T12:34:46Z Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus Cheng, Ming Soon Ho, Jia Shin Tan, Cheong Huat Wong, Jeslyn Pei Sze Ng, Lee Ching Toh, Chee-Seng School of Physical and Mathematical Sciences A sensitive membrane-based electrochemical nanobiosensor is developed for the detection of dengue type 2 virus (DENV-2) using nanoporous alumina-modified platinum electrode. Its sensing mechanism relies on the monitoring of electrode's Faradaic current response toward redox probe, ferrocenemethanol, which is sensitive toward the formation of immune complexes within the alumina nanochannels. Anti-DENV-2 monoclonal antibody (clone 3H5, isotype IgG) is used as the biorecognition element in this work. The stepwise additions of antibody, bovine serum albumin (BSA) and DENV-2 are characterized by differential pulse voltammetry (DPV). A low detection limit of 1 pfu mL−1 with linear range from 1 to 103 pfu mL−1 (R2 = 0.98) can be achieved by the nanobiosensor. The nanobiosensor is selective toward DENV-2 with insignificant cross reaction with non-specific viruses, Chikungunya virus, West Nile virus and dengue type 3 virus (DENV-3). Relative standard deviation (RSD) for triplicate analysis of 5.9% indicates an acceptable level of reproducibility. The first direct quantitation of DENV-2 concentration in whole mosquito vector is demonstrated using this electrochemical nanobiosensor. 2013-07-29T03:47:07Z 2019-12-06T19:53:38Z 2013-07-29T03:47:07Z 2019-12-06T19:53:38Z 2012 2012 Journal Article Cheng, M. S., Ho, J. S., Tan, C. H., Wong, J. P. S., Ng, L. C., & Toh, C.-S. (2012). Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus. Analytica Chimica Acta, 725, 74-80. 0003-2670 https://hdl.handle.net/10356/98333 http://hdl.handle.net/10220/12430 10.1016/j.aca.2012.03.017 en Analytica chimica acta © 2012 Elsevier B.V. |
| spellingShingle | Cheng, Ming Soon Ho, Jia Shin Tan, Cheong Huat Wong, Jeslyn Pei Sze Ng, Lee Ching Toh, Chee-Seng Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus |
| title | Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus |
| title_full | Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus |
| title_fullStr | Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus |
| title_full_unstemmed | Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus |
| title_short | Development of an electrochemical membrane-based nanobiosensor for ultrasensitive detection of dengue virus |
| title_sort | development of an electrochemical membrane based nanobiosensor for ultrasensitive detection of dengue virus |
| url | https://hdl.handle.net/10356/98333 http://hdl.handle.net/10220/12430 |
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