Biomimicking polysaccharide nanofibers promote vascular phenotypes : a potential application for vascular tissue engineering
The potential of electrospun pullulan/dextran (P/D) nanofibers (average diameter = 323 nm) for vascular tissue engineering applications is explored. The mechanical properties of the nanofibers are of the same order of magnitude as that of human arteries (Young's modulus ≈0.88 MPa; tensile stren...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/98408 http://hdl.handle.net/10220/17323 |
| _version_ | 1811689961070526464 |
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| author | Shi, Liya Aid, Rachida Visage, Catherine Le Chew, Sing Yian |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Shi, Liya Aid, Rachida Visage, Catherine Le Chew, Sing Yian |
| author_sort | Shi, Liya |
| collection | NTU |
| description | The potential of electrospun pullulan/dextran (P/D) nanofibers (average diameter = 323 nm) for vascular tissue engineering applications is explored. The mechanical properties of the nanofibers are of the same order of magnitude as that of human arteries (Young's modulus ≈0.88 MPa; tensile strength ≈0.35 MPa). It is demonstrated that the nanofiber topography enables cell adhesion and that the endothelial phenotype is maintained on the nanofibers. Moreover, P/D nanofibers support a stable confluent monolayer of endothelial cells over 14 d. SMCs seeded on nanofibers display similar levels of alpha smooth muscle actin and a lower proliferation rate than cells on 2D cultures. The observations suggest that nanofibers promote a shift to a quiescent contractile phenotype in SMCs. |
| first_indexed | 2024-10-01T05:56:25Z |
| format | Journal Article |
| id | ntu-10356/98408 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:56:25Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/984082020-03-07T11:35:38Z Biomimicking polysaccharide nanofibers promote vascular phenotypes : a potential application for vascular tissue engineering Shi, Liya Aid, Rachida Visage, Catherine Le Chew, Sing Yian School of Chemical and Biomedical Engineering DRNTU::Engineering::Bioengineering The potential of electrospun pullulan/dextran (P/D) nanofibers (average diameter = 323 nm) for vascular tissue engineering applications is explored. The mechanical properties of the nanofibers are of the same order of magnitude as that of human arteries (Young's modulus ≈0.88 MPa; tensile strength ≈0.35 MPa). It is demonstrated that the nanofiber topography enables cell adhesion and that the endothelial phenotype is maintained on the nanofibers. Moreover, P/D nanofibers support a stable confluent monolayer of endothelial cells over 14 d. SMCs seeded on nanofibers display similar levels of alpha smooth muscle actin and a lower proliferation rate than cells on 2D cultures. The observations suggest that nanofibers promote a shift to a quiescent contractile phenotype in SMCs. 2013-11-05T08:27:17Z 2019-12-06T19:54:56Z 2013-11-05T08:27:17Z 2019-12-06T19:54:56Z 2012 2012 Journal Article Shi, L., Aid, R., Visage, C. L., & Chew, S. Y. (2012). Biomimicking polysaccharide nanofibers promote vascular phenotypes : A potential application for vascular tissue engineering. Macromolecular bioscience, 12(3), 395-401. 1616-5187 https://hdl.handle.net/10356/98408 http://hdl.handle.net/10220/17323 10.1002/mabi.201100336 en Macromolecular bioscience |
| spellingShingle | DRNTU::Engineering::Bioengineering Shi, Liya Aid, Rachida Visage, Catherine Le Chew, Sing Yian Biomimicking polysaccharide nanofibers promote vascular phenotypes : a potential application for vascular tissue engineering |
| title | Biomimicking polysaccharide nanofibers promote vascular phenotypes : a potential application for vascular tissue engineering |
| title_full | Biomimicking polysaccharide nanofibers promote vascular phenotypes : a potential application for vascular tissue engineering |
| title_fullStr | Biomimicking polysaccharide nanofibers promote vascular phenotypes : a potential application for vascular tissue engineering |
| title_full_unstemmed | Biomimicking polysaccharide nanofibers promote vascular phenotypes : a potential application for vascular tissue engineering |
| title_short | Biomimicking polysaccharide nanofibers promote vascular phenotypes : a potential application for vascular tissue engineering |
| title_sort | biomimicking polysaccharide nanofibers promote vascular phenotypes a potential application for vascular tissue engineering |
| topic | DRNTU::Engineering::Bioengineering |
| url | https://hdl.handle.net/10356/98408 http://hdl.handle.net/10220/17323 |
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