Surface activation of poly(methyl methacrylate) by plasma treatment : stable antibody immobilization for microfluidic enzyme-linked immunosorbent assay
With the aim of obtaining stable antibody immobilization on the poly(methyl methacrylate), PMMA channel surface, PMMA substrates were activated with O2 plasma treatment to introduce surface polar groups on it. The plasma-treated PMMA surfaces were characterized using water contact angle measurement,...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/98444 http://hdl.handle.net/10220/12434 |
| _version_ | 1811696414794711040 |
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| author | Darain, Farzana Wahab, M. Abdul Tjin, Swee Chuan |
| author2 | School of Electrical and Electronic Engineering |
| author_facet | School of Electrical and Electronic Engineering Darain, Farzana Wahab, M. Abdul Tjin, Swee Chuan |
| author_sort | Darain, Farzana |
| collection | NTU |
| description | With the aim of obtaining stable antibody immobilization on the poly(methyl methacrylate), PMMA channel surface, PMMA substrates were activated with O2 plasma treatment to introduce surface polar groups on it. The plasma-treated PMMA surfaces were characterized using water contact angle measurement, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). It was observed that plasma treatment significantly improved the surface wettability with changing surface chemistry and topography. The strategy of immobilization of a model antibody, anti-goat IgG on plasma-treated PMMA involved two steps. First the plasma-treated PMMA was functionalized with (3-aminopropyl)thriethoxy silane, APTES off-chip which facilitated covalent capturing of antibody via a crosslinking agent in the inner surface of PMMA channel in the second step. The antibody immobilization on plasma-treated PMMA was also confirmed using AFM, XPS, and fluorescence microscopy. The anti-IgG covalently captured on channel surface was evaluated with sandwich ELISA protocol on-chip using fluorescence microscopy. The observed results demonstrate that this technique could be extended to integrate the current diagnostic techniques into the plastic chip for important biomarker diagnosis. |
| first_indexed | 2024-10-01T07:38:59Z |
| format | Journal Article |
| id | ntu-10356/98444 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:38:59Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/984442020-03-07T14:00:30Z Surface activation of poly(methyl methacrylate) by plasma treatment : stable antibody immobilization for microfluidic enzyme-linked immunosorbent assay Darain, Farzana Wahab, M. Abdul Tjin, Swee Chuan School of Electrical and Electronic Engineering Photonics Research Centre DRNTU::Engineering::Electrical and electronic engineering With the aim of obtaining stable antibody immobilization on the poly(methyl methacrylate), PMMA channel surface, PMMA substrates were activated with O2 plasma treatment to introduce surface polar groups on it. The plasma-treated PMMA surfaces were characterized using water contact angle measurement, atomic force microscopy (AFM), and X-ray photoelectron spectroscopy (XPS). It was observed that plasma treatment significantly improved the surface wettability with changing surface chemistry and topography. The strategy of immobilization of a model antibody, anti-goat IgG on plasma-treated PMMA involved two steps. First the plasma-treated PMMA was functionalized with (3-aminopropyl)thriethoxy silane, APTES off-chip which facilitated covalent capturing of antibody via a crosslinking agent in the inner surface of PMMA channel in the second step. The antibody immobilization on plasma-treated PMMA was also confirmed using AFM, XPS, and fluorescence microscopy. The anti-IgG covalently captured on channel surface was evaluated with sandwich ELISA protocol on-chip using fluorescence microscopy. The observed results demonstrate that this technique could be extended to integrate the current diagnostic techniques into the plastic chip for important biomarker diagnosis. 2013-07-29T03:59:59Z 2019-12-06T19:55:18Z 2013-07-29T03:59:59Z 2019-12-06T19:55:18Z 2012 2012 Journal Article Darain, F., Wahab, M. A., & Tjin, S. C. (2012). Surface Activation of Poly(Methyl methacrylate) by Plasma Treatment: Stable Antibody Immobilization for Microfluidic Enzyme-Linked Immunosorbent Assay. Analytical Letters, 45(17), 2569-2579. https://hdl.handle.net/10356/98444 http://hdl.handle.net/10220/12434 10.1080/00032719.2012.698673 en Analytical letters © 2012 Taylor & Francis Group, LLC. |
| spellingShingle | DRNTU::Engineering::Electrical and electronic engineering Darain, Farzana Wahab, M. Abdul Tjin, Swee Chuan Surface activation of poly(methyl methacrylate) by plasma treatment : stable antibody immobilization for microfluidic enzyme-linked immunosorbent assay |
| title | Surface activation of poly(methyl methacrylate) by plasma treatment : stable antibody immobilization for microfluidic enzyme-linked immunosorbent assay |
| title_full | Surface activation of poly(methyl methacrylate) by plasma treatment : stable antibody immobilization for microfluidic enzyme-linked immunosorbent assay |
| title_fullStr | Surface activation of poly(methyl methacrylate) by plasma treatment : stable antibody immobilization for microfluidic enzyme-linked immunosorbent assay |
| title_full_unstemmed | Surface activation of poly(methyl methacrylate) by plasma treatment : stable antibody immobilization for microfluidic enzyme-linked immunosorbent assay |
| title_short | Surface activation of poly(methyl methacrylate) by plasma treatment : stable antibody immobilization for microfluidic enzyme-linked immunosorbent assay |
| title_sort | surface activation of poly methyl methacrylate by plasma treatment stable antibody immobilization for microfluidic enzyme linked immunosorbent assay |
| topic | DRNTU::Engineering::Electrical and electronic engineering |
| url | https://hdl.handle.net/10356/98444 http://hdl.handle.net/10220/12434 |
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