Advanced immobilization and amplification for high performance protein chips
With the success of high-throughput DNA microarrays, protein biochips have been intensively investigated and broadly used in bioscience research, clinic diagnosis, drug discovery, and other applications. However, there is great need to significantly improve the sensitivity of protein chips, especial...
| Main Authors: | , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/98462 http://hdl.handle.net/10220/12433 |
| _version_ | 1811692999329972224 |
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| author | Liu, Yingshuai Li, Chang Ming |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Liu, Yingshuai Li, Chang Ming |
| author_sort | Liu, Yingshuai |
| collection | NTU |
| description | With the success of high-throughput DNA microarrays, protein biochips have been intensively investigated and broadly used in bioscience research, clinic diagnosis, drug discovery, and other applications. However, there is great need to significantly improve the sensitivity of protein chips, especially in early diagnosis. A major challenge of improving sensitivity is that protein detection does not have an effective amplification method, such as PCR for DNA microarrays. Construction of unique biofilms for efficient immobilization of protein probes and innovation of new amplification schemes could play a critical role in performance improvement of protein biochips. With dramatic developments in microfabrication, nanotechnologies, and biotechnologies, enormous progress has been made, particularly in improving biosensing sensitivity. This article reviews new advances in protein biochip technologies with emphasis on novel approaches for efficient probe immobilization and nanomaterials-assisted signal amplification for high performance protein chips. Prominent progress in integration of protein microarrays with microfluidic platforms is briefly discussed. The major challenges and perspectives on the future of protein biochips are also addressed. |
| first_indexed | 2024-10-01T06:44:42Z |
| format | Journal Article |
| id | ntu-10356/98462 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:44:42Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/984622020-03-07T11:35:39Z Advanced immobilization and amplification for high performance protein chips Liu, Yingshuai Li, Chang Ming School of Chemical and Biomedical Engineering Centre for Advanced Bionanosystems With the success of high-throughput DNA microarrays, protein biochips have been intensively investigated and broadly used in bioscience research, clinic diagnosis, drug discovery, and other applications. However, there is great need to significantly improve the sensitivity of protein chips, especially in early diagnosis. A major challenge of improving sensitivity is that protein detection does not have an effective amplification method, such as PCR for DNA microarrays. Construction of unique biofilms for efficient immobilization of protein probes and innovation of new amplification schemes could play a critical role in performance improvement of protein biochips. With dramatic developments in microfabrication, nanotechnologies, and biotechnologies, enormous progress has been made, particularly in improving biosensing sensitivity. This article reviews new advances in protein biochip technologies with emphasis on novel approaches for efficient probe immobilization and nanomaterials-assisted signal amplification for high performance protein chips. Prominent progress in integration of protein microarrays with microfluidic platforms is briefly discussed. The major challenges and perspectives on the future of protein biochips are also addressed. 2013-07-29T03:57:30Z 2019-12-06T19:55:28Z 2013-07-29T03:57:30Z 2019-12-06T19:55:28Z 2012 2012 Journal Article Liu, Y., & Li, C. M. (2012). Advanced Immobilization and Amplification for High Performance Protein Chips. Analytical Letters, 45(2-3), 130-155. https://hdl.handle.net/10356/98462 http://hdl.handle.net/10220/12433 10.1080/00032719.2011.633187 en Analytical letters © 2012 Taylor & Francis Group, LLC. |
| spellingShingle | Liu, Yingshuai Li, Chang Ming Advanced immobilization and amplification for high performance protein chips |
| title | Advanced immobilization and amplification for high performance protein chips |
| title_full | Advanced immobilization and amplification for high performance protein chips |
| title_fullStr | Advanced immobilization and amplification for high performance protein chips |
| title_full_unstemmed | Advanced immobilization and amplification for high performance protein chips |
| title_short | Advanced immobilization and amplification for high performance protein chips |
| title_sort | advanced immobilization and amplification for high performance protein chips |
| url | https://hdl.handle.net/10356/98462 http://hdl.handle.net/10220/12433 |
| work_keys_str_mv | AT liuyingshuai advancedimmobilizationandamplificationforhighperformanceproteinchips AT lichangming advancedimmobilizationandamplificationforhighperformanceproteinchips |