The imminent role of protein engineering in synthetic biology
Protein engineering has for decades been a powerful tool in biotechnology for generating vast numbers of useful enzymes for industrial applications. Today, protein engineering has a crucial role in advancing the emerging field of synthetic biology, where metabolic engineering efforts alone are insuf...
| Main Authors: | , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/98899 http://hdl.handle.net/10220/12702 |
| _version_ | 1811687220537458688 |
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| author | Foo, Jee Loon Ching, Chi Bun Chang, Matthew Wook Leong, Susanna Su Jan |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Foo, Jee Loon Ching, Chi Bun Chang, Matthew Wook Leong, Susanna Su Jan |
| author_sort | Foo, Jee Loon |
| collection | NTU |
| description | Protein engineering has for decades been a powerful tool in biotechnology for generating vast numbers of useful enzymes for industrial applications. Today, protein engineering has a crucial role in advancing the emerging field of synthetic biology, where metabolic engineering efforts alone are insufficient to maximize the full potential of synthetic biology. This article reviews the advancements in protein engineering techniques for improving biocatalytic properties to optimize engineered pathways in host systems, which are instrumental to achieve high titer production of target molecules. We also discuss the specific means by which protein engineering has improved metabolic engineering efforts and provide our assessment on its potential to continue to advance biology engineering as a whole. |
| first_indexed | 2024-10-01T05:12:51Z |
| format | Journal Article |
| id | ntu-10356/98899 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:12:51Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/988992020-03-07T11:35:38Z The imminent role of protein engineering in synthetic biology Foo, Jee Loon Ching, Chi Bun Chang, Matthew Wook Leong, Susanna Su Jan School of Chemical and Biomedical Engineering Protein engineering has for decades been a powerful tool in biotechnology for generating vast numbers of useful enzymes for industrial applications. Today, protein engineering has a crucial role in advancing the emerging field of synthetic biology, where metabolic engineering efforts alone are insufficient to maximize the full potential of synthetic biology. This article reviews the advancements in protein engineering techniques for improving biocatalytic properties to optimize engineered pathways in host systems, which are instrumental to achieve high titer production of target molecules. We also discuss the specific means by which protein engineering has improved metabolic engineering efforts and provide our assessment on its potential to continue to advance biology engineering as a whole. 2013-08-01T02:04:48Z 2019-12-06T20:00:55Z 2013-08-01T02:04:48Z 2019-12-06T20:00:55Z 2012 2012 Journal Article Foo, J. L., Ching, C. B., Chang, M. W.,& Leong, S. S. J. (2012). The imminent role of protein engineering in synthetic biology. Biotechnology Advances, 30(3), 541-549. 0734-9750 https://hdl.handle.net/10356/98899 http://hdl.handle.net/10220/12702 10.1016/j.biotechadv.2011.09.008 en Biotechnology advances |
| spellingShingle | Foo, Jee Loon Ching, Chi Bun Chang, Matthew Wook Leong, Susanna Su Jan The imminent role of protein engineering in synthetic biology |
| title | The imminent role of protein engineering in synthetic biology |
| title_full | The imminent role of protein engineering in synthetic biology |
| title_fullStr | The imminent role of protein engineering in synthetic biology |
| title_full_unstemmed | The imminent role of protein engineering in synthetic biology |
| title_short | The imminent role of protein engineering in synthetic biology |
| title_sort | imminent role of protein engineering in synthetic biology |
| url | https://hdl.handle.net/10356/98899 http://hdl.handle.net/10220/12702 |
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