Temporal control over cellular targeting through hybridization of folate-targeted dendrimers and PEG-PLA nanoparticles
Polymeric nanoparticles (NPs) and dendrimers are two major classes of nanomaterials that have demonstrated great potential for targeted drug delivery. However, their targeting efficacy has not yet met clinical needs, largely because of a lack of control over their targeting kinetics, which often res...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/98957 http://hdl.handle.net/10220/12814 |
| _version_ | 1811688365105348608 |
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| author | Sunoqrot, Suhair Bae, Jin Woo Shyu, Kevin Liu, Ying Kim, Dong-Hwan Hong, Seungpyo Pearson, Ryan M. |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Sunoqrot, Suhair Bae, Jin Woo Shyu, Kevin Liu, Ying Kim, Dong-Hwan Hong, Seungpyo Pearson, Ryan M. |
| author_sort | Sunoqrot, Suhair |
| collection | NTU |
| description | Polymeric nanoparticles (NPs) and dendrimers are two major classes of nanomaterials that have demonstrated great potential for targeted drug delivery. However, their targeting efficacy has not yet met clinical needs, largely because of a lack of control over their targeting kinetics, which often results in rapid clearance and off-target drug delivery. To address this issue, we have designed a novel hybrid NP (nanohybrid) platform that allows targeting kinetics to be effectively controlled through hybridization of targeted dendrimers with polymeric NPs. Folate (FA)-targeted generation 4 poly(amidoamine) dendrimers were encapsulated into poly(ethylene glycol)-b-poly(d,l-lactide) (PEG-PLA) NPs using a double emulsion method, forming nanohybrids with a uniform size (100 nm in diameter) at high encapsulation efficiencies (69–85%). Targeted dendrimers encapsulated within the NPs selectively interacted with FA receptor (FR)-overexpressing KB cells upon release in a temporally controlled manner. The targeting kinetics of the nanohybrids were modulated using three different molecular weights (MW) of the PLA block (23, 30, and 45 kDa). The release rates of the dendrimers from the nanohybrids were inversely proportional to the MW of the PLA block, which dictated their binding and internalization kinetics with KB cells. Our results provide evidence that selective cellular interactions can be kinetically controlled by the nanohybrid design, which can potentially enhance targeting efficacy of nanocarriers. |
| first_indexed | 2024-10-01T05:31:03Z |
| format | Journal Article |
| id | ntu-10356/98957 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:31:03Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/989572022-02-16T16:28:54Z Temporal control over cellular targeting through hybridization of folate-targeted dendrimers and PEG-PLA nanoparticles Sunoqrot, Suhair Bae, Jin Woo Shyu, Kevin Liu, Ying Kim, Dong-Hwan Hong, Seungpyo Pearson, Ryan M. School of Chemical and Biomedical Engineering Polymeric nanoparticles (NPs) and dendrimers are two major classes of nanomaterials that have demonstrated great potential for targeted drug delivery. However, their targeting efficacy has not yet met clinical needs, largely because of a lack of control over their targeting kinetics, which often results in rapid clearance and off-target drug delivery. To address this issue, we have designed a novel hybrid NP (nanohybrid) platform that allows targeting kinetics to be effectively controlled through hybridization of targeted dendrimers with polymeric NPs. Folate (FA)-targeted generation 4 poly(amidoamine) dendrimers were encapsulated into poly(ethylene glycol)-b-poly(d,l-lactide) (PEG-PLA) NPs using a double emulsion method, forming nanohybrids with a uniform size (100 nm in diameter) at high encapsulation efficiencies (69–85%). Targeted dendrimers encapsulated within the NPs selectively interacted with FA receptor (FR)-overexpressing KB cells upon release in a temporally controlled manner. The targeting kinetics of the nanohybrids were modulated using three different molecular weights (MW) of the PLA block (23, 30, and 45 kDa). The release rates of the dendrimers from the nanohybrids were inversely proportional to the MW of the PLA block, which dictated their binding and internalization kinetics with KB cells. Our results provide evidence that selective cellular interactions can be kinetically controlled by the nanohybrid design, which can potentially enhance targeting efficacy of nanocarriers. 2013-08-02T02:21:51Z 2019-12-06T20:01:29Z 2013-08-02T02:21:51Z 2019-12-06T20:01:29Z 2012 2012 Journal Article Sunoqrot, S., Bae, J. W., Pearson, R. M., Shyu, K., Liu, Y., Kim, D. H.,& Hong, S. (2012). Temporal Control over Cellular Targeting through Hybridization of Folate-targeted Dendrimers and PEG-PLA Nanoparticles. Biomacromolecules, 13(4), 1223-1230. https://hdl.handle.net/10356/98957 http://hdl.handle.net/10220/12814 10.1021/bm300316n 22439905 en Biomacromolecules |
| spellingShingle | Sunoqrot, Suhair Bae, Jin Woo Shyu, Kevin Liu, Ying Kim, Dong-Hwan Hong, Seungpyo Pearson, Ryan M. Temporal control over cellular targeting through hybridization of folate-targeted dendrimers and PEG-PLA nanoparticles |
| title | Temporal control over cellular targeting through hybridization of folate-targeted dendrimers and PEG-PLA nanoparticles |
| title_full | Temporal control over cellular targeting through hybridization of folate-targeted dendrimers and PEG-PLA nanoparticles |
| title_fullStr | Temporal control over cellular targeting through hybridization of folate-targeted dendrimers and PEG-PLA nanoparticles |
| title_full_unstemmed | Temporal control over cellular targeting through hybridization of folate-targeted dendrimers and PEG-PLA nanoparticles |
| title_short | Temporal control over cellular targeting through hybridization of folate-targeted dendrimers and PEG-PLA nanoparticles |
| title_sort | temporal control over cellular targeting through hybridization of folate targeted dendrimers and peg pla nanoparticles |
| url | https://hdl.handle.net/10356/98957 http://hdl.handle.net/10220/12814 |
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