Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites
A common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. Here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance th...
| Main Authors: | , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/98974 http://hdl.handle.net/10220/18428 |
| _version_ | 1811681511478394880 |
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| author | Penesyan, Anahit Ballestriero, Francesco Daim, Malak Kjelleberg, Staffan Thomas, Torsten Egan, Suhelen |
| author_facet | Penesyan, Anahit Ballestriero, Francesco Daim, Malak Kjelleberg, Staffan Thomas, Torsten Egan, Suhelen |
| author_sort | Penesyan, Anahit |
| collection | NTU |
| description | A common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. Here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance the screening efficiency, by increasing the “hit-rate” and unmasking multiple activities from the same bacterial source. |
| first_indexed | 2024-10-01T03:42:06Z |
| format | Journal Article |
| id | ntu-10356/98974 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T03:42:06Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/989742022-02-16T16:31:18Z Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites Penesyan, Anahit Ballestriero, Francesco Daim, Malak Kjelleberg, Staffan Thomas, Torsten Egan, Suhelen DRNTU::Science::Biological sciences A common limitation for the identification of novel activities from functional (meta) genomic screens is the low number of active clones detected relative to the number of clones screened. Here we demonstrate that constructing libraries with strains known to produce bioactives can greatly enhance the screening efficiency, by increasing the “hit-rate” and unmasking multiple activities from the same bacterial source. Published version 2014-01-10T02:49:25Z 2019-12-06T20:01:51Z 2014-01-10T02:49:25Z 2019-12-06T20:01:51Z 2013 2013 Journal Article Penesyan, A., Ballestriero, F., Daim, M., Kjelleberg, S., Thomas, T., & Egan, S. (2013). Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites. Marine drugs, 11(1), 40-49. 1660-3397 https://hdl.handle.net/10356/98974 http://hdl.handle.net/10220/18428 10.3390/md11010040 23271424 en Marine drugs © 2013 The Authors (published by MDPI). This paper was published in Marine Drugs and is made available as an electronic reprint (preprint) with permission of the authors. The paper can be found at the following official DOI: [http://dx.doi.org/10.3390/md11010040]. One print or electronic copy may be made for personal use only. Systematic or multiple reproduction, distribution to multiple locations via electronic or other means, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper is prohibited and is subject to penalties under law. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Penesyan, Anahit Ballestriero, Francesco Daim, Malak Kjelleberg, Staffan Thomas, Torsten Egan, Suhelen Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites |
| title | Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites |
| title_full | Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites |
| title_fullStr | Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites |
| title_full_unstemmed | Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites |
| title_short | Assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites |
| title_sort | assessing the effectiveness of functional genetic screens for the identification of bioactive metabolites |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/98974 http://hdl.handle.net/10220/18428 |
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