Size influences the cytotoxicity of poly (lactic-co-glycolic acid) (PLGA) and titanium dioxide (TiO2) nanoparticles
The aim of this study is to uncover the size influence of poly (lactic-co-glycolic acid) (PLGA) and titanium dioxide (TiO2) nanoparticles on their potential cytotoxicity. PLGA and TiO2 nanoparticles of three different sizes were thoroughly characterized before in vitro cytotoxic tests which included...
| Main Authors: | , , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2013
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| Online Access: | https://hdl.handle.net/10356/98977 http://hdl.handle.net/10220/12617 |
| _version_ | 1811686810607157248 |
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| author | Xiong, Sijing George, Saji Yu, Haiyang Damoiseaux, Robert France, Bryan Ng, Kee Woei Loo, Say Chye Joachim |
| author2 | School of Materials Science & Engineering |
| author_facet | School of Materials Science & Engineering Xiong, Sijing George, Saji Yu, Haiyang Damoiseaux, Robert France, Bryan Ng, Kee Woei Loo, Say Chye Joachim |
| author_sort | Xiong, Sijing |
| collection | NTU |
| description | The aim of this study is to uncover the size influence of poly (lactic-co-glycolic acid) (PLGA) and titanium dioxide (TiO2) nanoparticles on their potential cytotoxicity. PLGA and TiO2 nanoparticles of three different sizes were thoroughly characterized before in vitro cytotoxic tests which included viability, generation of reactive oxygen species (ROS), mitochondrial depolarization, integrity of plasma membrane, intracellular calcium influx and cytokine release. Size-dependent cytotoxic effect was observed in both RAW264.7 cells and BEAS-2B cells after cells were incubated with PLGA or TiO2 nanoparticles for 24 h. Although PLGA nanoparticles did not trigger significantly lethal toxicity up to a concentration of 300 μg/ml, the TNF-α release after the stimulation of PLGA nanoparticles should not be ignored especially in clinical applications. Relatively more toxic TiO2 nanoparticles triggered cell death, ROS generation, mitochondrial depolarization, plasma membrane damage, intracellular calcium concentration increase and size-dependent TNF-α release, especially at a concentration higher than 100 μg/ml. These cytotoxic effects could be due to the size-dependent interaction between nanoparticles and biomolecules, as smaller particles tend to adsorb more biomolecules. In summary, we demonstrated that the ability of protein adsorption could be an important paradigm to predict the in vitro cytotoxicity of nanoparticles, especially for low toxic nanomaterials such as PLGA and TiO2 nanoparticles. |
| first_indexed | 2024-10-01T05:06:20Z |
| format | Journal Article |
| id | ntu-10356/98977 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T05:06:20Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/989772023-07-14T15:54:32Z Size influences the cytotoxicity of poly (lactic-co-glycolic acid) (PLGA) and titanium dioxide (TiO2) nanoparticles Xiong, Sijing George, Saji Yu, Haiyang Damoiseaux, Robert France, Bryan Ng, Kee Woei Loo, Say Chye Joachim School of Materials Science & Engineering The aim of this study is to uncover the size influence of poly (lactic-co-glycolic acid) (PLGA) and titanium dioxide (TiO2) nanoparticles on their potential cytotoxicity. PLGA and TiO2 nanoparticles of three different sizes were thoroughly characterized before in vitro cytotoxic tests which included viability, generation of reactive oxygen species (ROS), mitochondrial depolarization, integrity of plasma membrane, intracellular calcium influx and cytokine release. Size-dependent cytotoxic effect was observed in both RAW264.7 cells and BEAS-2B cells after cells were incubated with PLGA or TiO2 nanoparticles for 24 h. Although PLGA nanoparticles did not trigger significantly lethal toxicity up to a concentration of 300 μg/ml, the TNF-α release after the stimulation of PLGA nanoparticles should not be ignored especially in clinical applications. Relatively more toxic TiO2 nanoparticles triggered cell death, ROS generation, mitochondrial depolarization, plasma membrane damage, intracellular calcium concentration increase and size-dependent TNF-α release, especially at a concentration higher than 100 μg/ml. These cytotoxic effects could be due to the size-dependent interaction between nanoparticles and biomolecules, as smaller particles tend to adsorb more biomolecules. In summary, we demonstrated that the ability of protein adsorption could be an important paradigm to predict the in vitro cytotoxicity of nanoparticles, especially for low toxic nanomaterials such as PLGA and TiO2 nanoparticles. Accepted version 2013-07-31T06:22:57Z 2019-12-06T20:01:54Z 2013-07-31T06:22:57Z 2019-12-06T20:01:54Z 2012 2012 Journal Article Xiong, S., George, S., Yu, H., Damoiseaux, R., France, B., Ng, K. W., & Loo, S. C. J. (2013). Size influences the cytotoxicity of poly (lactic-co-glycolic acid) (PLGA) and titanium dioxide (TiO2) nanoparticles. Archives of Toxicology, 87(6), 1075-1086. https://hdl.handle.net/10356/98977 http://hdl.handle.net/10220/12617 10.1007/s00204-012-0938-8 en Archives of toxicology © 2012 Springer-Verlag. This is the author created version of a work that has been peer reviewed and accepted for publication by Archives of toxicology, Springer-Verlag. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [http://dx.doi.org/10.1007/s00204-012-0938-8]. application/pdf |
| spellingShingle | Xiong, Sijing George, Saji Yu, Haiyang Damoiseaux, Robert France, Bryan Ng, Kee Woei Loo, Say Chye Joachim Size influences the cytotoxicity of poly (lactic-co-glycolic acid) (PLGA) and titanium dioxide (TiO2) nanoparticles |
| title | Size influences the cytotoxicity of poly (lactic-co-glycolic acid) (PLGA) and titanium dioxide (TiO2) nanoparticles |
| title_full | Size influences the cytotoxicity of poly (lactic-co-glycolic acid) (PLGA) and titanium dioxide (TiO2) nanoparticles |
| title_fullStr | Size influences the cytotoxicity of poly (lactic-co-glycolic acid) (PLGA) and titanium dioxide (TiO2) nanoparticles |
| title_full_unstemmed | Size influences the cytotoxicity of poly (lactic-co-glycolic acid) (PLGA) and titanium dioxide (TiO2) nanoparticles |
| title_short | Size influences the cytotoxicity of poly (lactic-co-glycolic acid) (PLGA) and titanium dioxide (TiO2) nanoparticles |
| title_sort | size influences the cytotoxicity of poly lactic co glycolic acid plga and titanium dioxide tio2 nanoparticles |
| url | https://hdl.handle.net/10356/98977 http://hdl.handle.net/10220/12617 |
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