The in vivo biofilm
Bacteria can grow and proliferate either as single, independent cells or organized in aggregates commonly referred to as biofilms. When bacteria succeed in forming a biofilm within the human host, the infection often becomes very resistant to treatment and can develop into a chronic state. Biofilms...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/99289 http://hdl.handle.net/10220/17367 |
| _version_ | 1826129460852686848 |
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| author | Jensen, Peter Østrup Høiby, Niels Bjarnsholt, Thomas Alhede, Maria Alhede, Morten Eickhardt-Sørensen, Steffen R. Moser, Claus Kühl, Michael |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Jensen, Peter Østrup Høiby, Niels Bjarnsholt, Thomas Alhede, Maria Alhede, Morten Eickhardt-Sørensen, Steffen R. Moser, Claus Kühl, Michael |
| author_sort | Jensen, Peter Østrup |
| collection | NTU |
| description | Bacteria can grow and proliferate either as single, independent cells or organized in aggregates commonly referred to as biofilms. When bacteria succeed in forming a biofilm within the human host, the infection often becomes very resistant to treatment and can develop into a chronic state. Biofilms have been studied for decades using various in vitro models, but it remains debatable whether such in vitro biofilms actually resemble in vivo biofilms in chronic infections. In vivo biofilms share several structural characteristics that differ from most in vitro biofilms. Additionally, the in vivo experimental time span and presence of host defenses differ from chronic infections and the chemical microenvironment of both in vivo and in vitro biofilms is seldom taken into account. In this review, we discuss why the current in vitro models of biofilms might be limited for describing infectious biofilms, and we suggest new strategies for improving this discrepancy. |
| first_indexed | 2024-10-01T07:40:39Z |
| format | Journal Article |
| id | ntu-10356/99289 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T07:40:39Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/992892020-03-07T12:18:13Z The in vivo biofilm Jensen, Peter Østrup Høiby, Niels Bjarnsholt, Thomas Alhede, Maria Alhede, Morten Eickhardt-Sørensen, Steffen R. Moser, Claus Kühl, Michael School of Biological Sciences DRNTU::Science::Biological sciences Bacteria can grow and proliferate either as single, independent cells or organized in aggregates commonly referred to as biofilms. When bacteria succeed in forming a biofilm within the human host, the infection often becomes very resistant to treatment and can develop into a chronic state. Biofilms have been studied for decades using various in vitro models, but it remains debatable whether such in vitro biofilms actually resemble in vivo biofilms in chronic infections. In vivo biofilms share several structural characteristics that differ from most in vitro biofilms. Additionally, the in vivo experimental time span and presence of host defenses differ from chronic infections and the chemical microenvironment of both in vivo and in vitro biofilms is seldom taken into account. In this review, we discuss why the current in vitro models of biofilms might be limited for describing infectious biofilms, and we suggest new strategies for improving this discrepancy. 2013-11-07T06:19:43Z 2019-12-06T20:05:26Z 2013-11-07T06:19:43Z 2019-12-06T20:05:26Z 2013 2013 Journal Article Bjarnsholt, T., Alhede, M., Alhede, M., Eickhardt-Sørensen, S. R., Moser, C., Kühl, M., et al. (2013). The in vivo biofilm. Trends in Microbiology, 21(9), 466-474. 0966-842X https://hdl.handle.net/10356/99289 http://hdl.handle.net/10220/17367 10.1016/j.tim.2013.06.002 en Trends in microbiology |
| spellingShingle | DRNTU::Science::Biological sciences Jensen, Peter Østrup Høiby, Niels Bjarnsholt, Thomas Alhede, Maria Alhede, Morten Eickhardt-Sørensen, Steffen R. Moser, Claus Kühl, Michael The in vivo biofilm |
| title | The in vivo biofilm |
| title_full | The in vivo biofilm |
| title_fullStr | The in vivo biofilm |
| title_full_unstemmed | The in vivo biofilm |
| title_short | The in vivo biofilm |
| title_sort | in vivo biofilm |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/99289 http://hdl.handle.net/10220/17367 |
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