A mixed co-culture of mesenchymal stem cells and transgenic chondrocytes in alginate hydrogel for cartilage tissue engineering
To regenerate articular cartilage tissue from degeneration and trauma, synovial mesenchymal stem cells (SMSCs) were used in this study as therapeutic progenitor cells to induce therapeutic chondrogenesis. To accomplish this, chondrocytes pre-transduced with adenoviral vectors carrying the transformi...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/99295 http://hdl.handle.net/10220/17313 |
| _version_ | 1826124739379200000 |
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| author | Zhang, Feng Su, Kai Fang, Yu Sandhya, Swaminathan Wang, Dong-An |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Zhang, Feng Su, Kai Fang, Yu Sandhya, Swaminathan Wang, Dong-An |
| author_sort | Zhang, Feng |
| collection | NTU |
| description | To regenerate articular cartilage tissue from degeneration and trauma, synovial mesenchymal stem cells (SMSCs) were used in this study as therapeutic progenitor cells to induce therapeutic chondrogenesis. To accomplish this, chondrocytes pre-transduced with adenoviral vectors carrying the transforming growth factor (TGF) β3 gene were selected as transgenic companion cells and co-cultured side-by-side with SMSCs in a 3D environment to provide chondrogenic growth factors in situ. We adopted a mixed co-culture strategy for this purpose. Transgenic delivery of TGF-β3 in chondrocytes was performed via recombinant adenoviral vectors. The mixed co-culture of SMSCs and transgenic chondrocytes was produced in alginate gel constructs. Gene expression in both SMSCs and chondrocytes were characterized. Biochemical assays in vitro and in vivo showed that release of TGF-ß3 from transgenic chondrocytes not only induced SMSC differentiation into chondrocytic cells but also preserved the chondrocytic phenotype of chondrocytes from suspected dedifferentiation. As a result, this mixed co-culture strategy in conjunction with TGF-ß3 gene delivery could be a promising approach in cartilage tissue engineering. |
| first_indexed | 2024-10-01T06:25:17Z |
| format | Journal Article |
| id | ntu-10356/99295 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:25:17Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/992952020-03-07T11:35:31Z A mixed co-culture of mesenchymal stem cells and transgenic chondrocytes in alginate hydrogel for cartilage tissue engineering Zhang, Feng Su, Kai Fang, Yu Sandhya, Swaminathan Wang, Dong-An School of Chemical and Biomedical Engineering DRNTU::Engineering::Chemical engineering::Biochemical engineering To regenerate articular cartilage tissue from degeneration and trauma, synovial mesenchymal stem cells (SMSCs) were used in this study as therapeutic progenitor cells to induce therapeutic chondrogenesis. To accomplish this, chondrocytes pre-transduced with adenoviral vectors carrying the transforming growth factor (TGF) β3 gene were selected as transgenic companion cells and co-cultured side-by-side with SMSCs in a 3D environment to provide chondrogenic growth factors in situ. We adopted a mixed co-culture strategy for this purpose. Transgenic delivery of TGF-β3 in chondrocytes was performed via recombinant adenoviral vectors. The mixed co-culture of SMSCs and transgenic chondrocytes was produced in alginate gel constructs. Gene expression in both SMSCs and chondrocytes were characterized. Biochemical assays in vitro and in vivo showed that release of TGF-ß3 from transgenic chondrocytes not only induced SMSC differentiation into chondrocytic cells but also preserved the chondrocytic phenotype of chondrocytes from suspected dedifferentiation. As a result, this mixed co-culture strategy in conjunction with TGF-ß3 gene delivery could be a promising approach in cartilage tissue engineering. 2013-11-05T07:38:23Z 2019-12-06T20:05:29Z 2013-11-05T07:38:23Z 2019-12-06T20:05:29Z 2012 2012 Journal Article Zhang, F., Su, K., Fang, Y., Sandhya, S., & Wang, D.-A. (2012). A mixed co-culture of mesenchymal stem cells and transgenic chondrocytes in alginate hydrogel for cartilage tissue engineering. Journal of tissue engineering and regenerative medicine, in press. 1932-6254 https://hdl.handle.net/10356/99295 http://hdl.handle.net/10220/17313 10.1002/term.1641 en Journal of tissue engineering and regenerative medicine |
| spellingShingle | DRNTU::Engineering::Chemical engineering::Biochemical engineering Zhang, Feng Su, Kai Fang, Yu Sandhya, Swaminathan Wang, Dong-An A mixed co-culture of mesenchymal stem cells and transgenic chondrocytes in alginate hydrogel for cartilage tissue engineering |
| title | A mixed co-culture of mesenchymal stem cells and transgenic chondrocytes in alginate hydrogel for cartilage tissue engineering |
| title_full | A mixed co-culture of mesenchymal stem cells and transgenic chondrocytes in alginate hydrogel for cartilage tissue engineering |
| title_fullStr | A mixed co-culture of mesenchymal stem cells and transgenic chondrocytes in alginate hydrogel for cartilage tissue engineering |
| title_full_unstemmed | A mixed co-culture of mesenchymal stem cells and transgenic chondrocytes in alginate hydrogel for cartilage tissue engineering |
| title_short | A mixed co-culture of mesenchymal stem cells and transgenic chondrocytes in alginate hydrogel for cartilage tissue engineering |
| title_sort | mixed co culture of mesenchymal stem cells and transgenic chondrocytes in alginate hydrogel for cartilage tissue engineering |
| topic | DRNTU::Engineering::Chemical engineering::Biochemical engineering |
| url | https://hdl.handle.net/10356/99295 http://hdl.handle.net/10220/17313 |
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