Proteomic analysis of temperature dependent extracellular proteins from Aspergillus fumigatus grown under solid-state culture condition
Fungal species of the genus Aspergillus are filamentous ubiquitous saprophytes that play a major role in lignocellulosic biomass recycling and also are considered as cell factories for the production of organic acids, pharmaceuticals, and industrially important enzymes. Analysis of extracellular sec...
| Main Authors: | , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2013
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| Online Access: | https://hdl.handle.net/10356/99296 http://hdl.handle.net/10220/17034 |
| _version_ | 1824455337065840640 |
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| author | Ravindran, Anita Sze, Siu Kwan Adav, Sunil S. |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Ravindran, Anita Sze, Siu Kwan Adav, Sunil S. |
| author_sort | Ravindran, Anita |
| collection | NTU |
| description | Fungal species of the genus Aspergillus are filamentous ubiquitous saprophytes that play a major role in lignocellulosic biomass recycling and also are considered as cell factories for the production of organic acids, pharmaceuticals, and industrially important enzymes. Analysis of extracellular secreted biomass degrading enzymes using complex lignocellulosic biomass as a substrate by solid-state fermentation could be a more practical approach to evaluate application of the enzymes for lignocellulosic biorefinery. This study isolated a fungal strain from compost, identified as Aspergillus fumigatus, and further analyzed it for lignocellulolytic enzymes at different temperatures using label free quantitative proteomics. The profile of secretome composition discovered cellulases, hemicellulases, lignin degrading proteins, peptidases and proteases, and transport and hypothetical proteins; while protein abundances and further their hierarchical clustering analysis revealed temperature dependent expression of these enzymes during solid-state fermentation of sawdust. The enzyme activities and protein abundances as determined by exponentially modified protein abundance index (emPAI) indicated the maximum activities at the range of 40–50 °C, demonstrating the thermophilic nature of the isolate A. fumigatus LF9. Characterization of the thermostability of secretome suggested the potential of the isolated fungal strain in the production of thermophilic biomass degrading enzymes for industrial application. |
| first_indexed | 2025-02-19T03:36:36Z |
| format | Journal Article |
| id | ntu-10356/99296 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:36:36Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/992962020-03-07T12:18:14Z Proteomic analysis of temperature dependent extracellular proteins from Aspergillus fumigatus grown under solid-state culture condition Ravindran, Anita Sze, Siu Kwan Adav, Sunil S. School of Biological Sciences DRNTU::Science::Biological sciences::Microbiology Fungal species of the genus Aspergillus are filamentous ubiquitous saprophytes that play a major role in lignocellulosic biomass recycling and also are considered as cell factories for the production of organic acids, pharmaceuticals, and industrially important enzymes. Analysis of extracellular secreted biomass degrading enzymes using complex lignocellulosic biomass as a substrate by solid-state fermentation could be a more practical approach to evaluate application of the enzymes for lignocellulosic biorefinery. This study isolated a fungal strain from compost, identified as Aspergillus fumigatus, and further analyzed it for lignocellulolytic enzymes at different temperatures using label free quantitative proteomics. The profile of secretome composition discovered cellulases, hemicellulases, lignin degrading proteins, peptidases and proteases, and transport and hypothetical proteins; while protein abundances and further their hierarchical clustering analysis revealed temperature dependent expression of these enzymes during solid-state fermentation of sawdust. The enzyme activities and protein abundances as determined by exponentially modified protein abundance index (emPAI) indicated the maximum activities at the range of 40–50 °C, demonstrating the thermophilic nature of the isolate A. fumigatus LF9. Characterization of the thermostability of secretome suggested the potential of the isolated fungal strain in the production of thermophilic biomass degrading enzymes for industrial application. 2013-10-30T04:03:01Z 2019-12-06T20:05:30Z 2013-10-30T04:03:01Z 2019-12-06T20:05:30Z 2013 2013 Journal Article Adav, S. S., Ravindran, A., & Sze, S. K. (2013). Proteomic Analysis of Temperature Dependent Extracellular Proteins from Aspergillus fumigatus Grown under Solid-State Culture Condition. Journal of Proteome Research, 12(6), 2715-2731. 1535-3893 https://hdl.handle.net/10356/99296 http://hdl.handle.net/10220/17034 10.1021/pr4000762 en Journal of proteome research |
| spellingShingle | DRNTU::Science::Biological sciences::Microbiology Ravindran, Anita Sze, Siu Kwan Adav, Sunil S. Proteomic analysis of temperature dependent extracellular proteins from Aspergillus fumigatus grown under solid-state culture condition |
| title | Proteomic analysis of temperature dependent extracellular proteins from Aspergillus fumigatus grown under solid-state culture condition |
| title_full | Proteomic analysis of temperature dependent extracellular proteins from Aspergillus fumigatus grown under solid-state culture condition |
| title_fullStr | Proteomic analysis of temperature dependent extracellular proteins from Aspergillus fumigatus grown under solid-state culture condition |
| title_full_unstemmed | Proteomic analysis of temperature dependent extracellular proteins from Aspergillus fumigatus grown under solid-state culture condition |
| title_short | Proteomic analysis of temperature dependent extracellular proteins from Aspergillus fumigatus grown under solid-state culture condition |
| title_sort | proteomic analysis of temperature dependent extracellular proteins from aspergillus fumigatus grown under solid state culture condition |
| topic | DRNTU::Science::Biological sciences::Microbiology |
| url | https://hdl.handle.net/10356/99296 http://hdl.handle.net/10220/17034 |
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