Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta
The term placenta is a highly vascularized tissue and is usually discarded upon birth. Our objective was to isolate clinically relevant quantities of fetal endothelial colony-forming cells (ECFCs) from human term placenta and to compare them to the well-established donor-matched umbilical cord blood...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/99343 http://hdl.handle.net/10220/24036 |
| _version_ | 1826110162575818752 |
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| author | Chan, Jerry Kok Yen Patel, Jatin Seppanen, Elke Chong, Mark S. K. Yeo, Julie S. L. Teo, Erin Y. L. Fisk, Nicholas M. Khosrotehrani, Kiarash |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Chan, Jerry Kok Yen Patel, Jatin Seppanen, Elke Chong, Mark S. K. Yeo, Julie S. L. Teo, Erin Y. L. Fisk, Nicholas M. Khosrotehrani, Kiarash |
| author_sort | Chan, Jerry Kok Yen |
| collection | NTU |
| description | The term placenta is a highly vascularized tissue and is usually discarded upon birth. Our objective was to isolate clinically relevant quantities of fetal endothelial colony-forming cells (ECFCs) from human term placenta and to compare them to the well-established donor-matched umbilical cord blood (UCB)-derived ECFCs. A sorting strategy was devised to enrich for CD45−CD34+CD31Lo cells prior to primary plating to obtain pure placental ECFCs (PL-ECFCs) upon culture. UCB-ECFCs were derived using a well-described assay. PL-ECFCs were fetal in origin and expressed the same cell surface markers as UCB-ECFCs. Most importantly, a single term placenta could yield as many ECFCs as 27 UCB donors. PL-ECFCs and UCB-ECFCs had similar in vitro and in vivo vessel forming capacities and restored mouse hind limb ischemia in similar proportions. Gene expression profiles were only minimally divergent between PL-ECFCs and UCB-ECFCs, probably reflecting a vascular source versus a circulating source. Finally, PL-ECFCs and UCB-ECFCs displayed similar hierarchies between high and low proliferative colonies. We report a robust strategy to isolate ECFCs from human term placentas based on their cell surface expression. This yielded much larger quantities of ECFCs than UCB, but the cells were comparable in immunophenotype, gene expression, and in vivo functional ability. We conclude that PL-ECFCs have significant bio-banking and clinical translatability potential. |
| first_indexed | 2024-10-01T02:29:54Z |
| format | Journal Article |
| id | ntu-10356/99343 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:29:54Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/993432022-02-16T16:29:21Z Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta Chan, Jerry Kok Yen Patel, Jatin Seppanen, Elke Chong, Mark S. K. Yeo, Julie S. L. Teo, Erin Y. L. Fisk, Nicholas M. Khosrotehrani, Kiarash School of Chemical and Biomedical Engineering DRNTU::Science::Chemistry::Biochemistry The term placenta is a highly vascularized tissue and is usually discarded upon birth. Our objective was to isolate clinically relevant quantities of fetal endothelial colony-forming cells (ECFCs) from human term placenta and to compare them to the well-established donor-matched umbilical cord blood (UCB)-derived ECFCs. A sorting strategy was devised to enrich for CD45−CD34+CD31Lo cells prior to primary plating to obtain pure placental ECFCs (PL-ECFCs) upon culture. UCB-ECFCs were derived using a well-described assay. PL-ECFCs were fetal in origin and expressed the same cell surface markers as UCB-ECFCs. Most importantly, a single term placenta could yield as many ECFCs as 27 UCB donors. PL-ECFCs and UCB-ECFCs had similar in vitro and in vivo vessel forming capacities and restored mouse hind limb ischemia in similar proportions. Gene expression profiles were only minimally divergent between PL-ECFCs and UCB-ECFCs, probably reflecting a vascular source versus a circulating source. Finally, PL-ECFCs and UCB-ECFCs displayed similar hierarchies between high and low proliferative colonies. We report a robust strategy to isolate ECFCs from human term placentas based on their cell surface expression. This yielded much larger quantities of ECFCs than UCB, but the cells were comparable in immunophenotype, gene expression, and in vivo functional ability. We conclude that PL-ECFCs have significant bio-banking and clinical translatability potential. 2014-10-15T02:51:21Z 2019-12-06T20:06:16Z 2014-10-15T02:51:21Z 2019-12-06T20:06:16Z 2013 2013 Journal Article Patel, J., Seppanen, E., Chong, M. S. K., Yeo, J. S. L., Teo, E. Y. L., Chan, J. K. Y., et al. (2013). Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta. Stem cells translational medicine, 2(11), 839-847. 2157-6564 https://hdl.handle.net/10356/99343 http://hdl.handle.net/10220/24036 10.5966/sctm.2013-0092 24106336 en Stem cells translational medicine © 2013 AlphaMed Press. |
| spellingShingle | DRNTU::Science::Chemistry::Biochemistry Chan, Jerry Kok Yen Patel, Jatin Seppanen, Elke Chong, Mark S. K. Yeo, Julie S. L. Teo, Erin Y. L. Fisk, Nicholas M. Khosrotehrani, Kiarash Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta |
| title | Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta |
| title_full | Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta |
| title_fullStr | Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta |
| title_full_unstemmed | Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta |
| title_short | Prospective surface marker-based isolation and expansion of fetal endothelial colony-forming cells from human term placenta |
| title_sort | prospective surface marker based isolation and expansion of fetal endothelial colony forming cells from human term placenta |
| topic | DRNTU::Science::Chemistry::Biochemistry |
| url | https://hdl.handle.net/10356/99343 http://hdl.handle.net/10220/24036 |
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