Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects
In this paper, two-dimensional flow field simulation was conducted to determine shear stresses and velocity profiles for bone tissue engineering in a rotating wall vessel bioreactor (RWVB). In addition, in vitro three-dimensional fabrication of tissue-engineered bones was carried out in optimized bi...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
| Published: |
2013
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| Online Access: | https://hdl.handle.net/10356/99463 http://hdl.handle.net/10220/12852 |
| _version_ | 1811676989209182208 |
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| author | Song, Kedong Wang, Hai Zhang, Bowen Lim, Mayasari Liu, YingChao Liu, Tianqing |
| author2 | School of Chemical and Biomedical Engineering |
| author_facet | School of Chemical and Biomedical Engineering Song, Kedong Wang, Hai Zhang, Bowen Lim, Mayasari Liu, YingChao Liu, Tianqing |
| author_sort | Song, Kedong |
| collection | NTU |
| description | In this paper, two-dimensional flow field simulation was conducted to determine shear stresses and velocity profiles for bone tissue engineering in a rotating wall vessel bioreactor (RWVB). In addition, in vitro three-dimensional fabrication of tissue-engineered bones was carried out in optimized bioreactor conditions, and in vivo implantation using fabricated bones was performed for segmental bone defects of Zelanian rabbits. The distribution of dynamic pressure, total pressure, shear stress, and velocity within the culture chamber was calculated for different scaffold locations. According to the simulation results, the dynamic pressure, velocity, and shear stress around the surface of cell-scaffold construction periodically changed at different locations of the RWVB, which could result in periodical stress stimulation for fabricated tissue constructs. However, overall shear stresses were relatively low, and the fluid velocities were uniform in the bioreactor. Our in vitro experiments showed that the number of cells cultured in the RWVB was five times higher than those cultured in a T-flask. The tissue-engineered bones grew very well in the RWVB. This study demonstrates that stress stimulation in an RWVB can be beneficial for cell/bio-derived bone constructs fabricated in an RWVB, with an application for repairing segmental bone defects. |
| first_indexed | 2024-10-01T02:30:14Z |
| format | Journal Article |
| id | ntu-10356/99463 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T02:30:14Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | ntu-10356/994632022-02-16T16:31:10Z Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects Song, Kedong Wang, Hai Zhang, Bowen Lim, Mayasari Liu, YingChao Liu, Tianqing School of Chemical and Biomedical Engineering In this paper, two-dimensional flow field simulation was conducted to determine shear stresses and velocity profiles for bone tissue engineering in a rotating wall vessel bioreactor (RWVB). In addition, in vitro three-dimensional fabrication of tissue-engineered bones was carried out in optimized bioreactor conditions, and in vivo implantation using fabricated bones was performed for segmental bone defects of Zelanian rabbits. The distribution of dynamic pressure, total pressure, shear stress, and velocity within the culture chamber was calculated for different scaffold locations. According to the simulation results, the dynamic pressure, velocity, and shear stress around the surface of cell-scaffold construction periodically changed at different locations of the RWVB, which could result in periodical stress stimulation for fabricated tissue constructs. However, overall shear stresses were relatively low, and the fluid velocities were uniform in the bioreactor. Our in vitro experiments showed that the number of cells cultured in the RWVB was five times higher than those cultured in a T-flask. The tissue-engineered bones grew very well in the RWVB. This study demonstrates that stress stimulation in an RWVB can be beneficial for cell/bio-derived bone constructs fabricated in an RWVB, with an application for repairing segmental bone defects. 2013-08-02T03:29:28Z 2019-12-06T20:07:47Z 2013-08-02T03:29:28Z 2019-12-06T20:07:47Z 2012 2012 Journal Article Song, K., Wang, H., Zhang, B., Lim, M., Liu, Y.,& Liu, T. (2013). Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects. Cell Stress and Chaperones, 18(2), 193-201. https://hdl.handle.net/10356/99463 http://hdl.handle.net/10220/12852 10.1007/s12192-012-0370-2 23054889 en Cell stress and chaperones |
| spellingShingle | Song, Kedong Wang, Hai Zhang, Bowen Lim, Mayasari Liu, YingChao Liu, Tianqing Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects |
| title | Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects |
| title_full | Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects |
| title_fullStr | Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects |
| title_full_unstemmed | Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects |
| title_short | Numerical simulation of fluid field and in vitro three-dimensional fabrication of tissue-engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects |
| title_sort | numerical simulation of fluid field and in vitro three dimensional fabrication of tissue engineered bones in a rotating bioreactor and in vivo implantation for repairing segmental bone defects |
| url | https://hdl.handle.net/10356/99463 http://hdl.handle.net/10220/12852 |
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