Comparison of single-stage and two-phase anaerobic sludge digestion systems : performance and microbial community dynamics
This study compared reactor performance and the respective microbial community dynamics in the conventional single-stage and 2-phase anaerobic digestion (AD) systems, treating municipal sludge to generate methane. The 2-phase system’s COD and VS reduction, and methane production could be maintained...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal Article |
| Language: | English |
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2014
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| Online Access: | https://hdl.handle.net/10356/99554 http://hdl.handle.net/10220/24056 |
| _version_ | 1811691877482627072 |
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| author | Maspolim, Yogananda Zhou, Yan Guo, Chenghong Xiao, Keke Ng, Wun Jern |
| author2 | School of Civil and Environmental Engineering |
| author_facet | School of Civil and Environmental Engineering Maspolim, Yogananda Zhou, Yan Guo, Chenghong Xiao, Keke Ng, Wun Jern |
| author_sort | Maspolim, Yogananda |
| collection | NTU |
| description | This study compared reactor performance and the respective microbial community dynamics in the conventional single-stage and 2-phase anaerobic digestion (AD) systems, treating municipal sludge to generate methane. The 2-phase system’s COD and VS reduction, and methane production could be maintained throughout the three HRTs tested (p = 0.05), which was associated with an increase in organic loading (30 d = 1.5 g COD L−1 d−1, 20 d = 2.2 g COD L−1 d−1 and 10 d = 3.5 g COD L−1 d−1); but this was not so in the single-stage system where it deteriorated at HRT of 10 d (p = 0.05) due to impairment of particulate COD reduction. qPCR, DGGE and the subsequent phylogenetic analysis revealed that microbial adaptation occurred as the seed sludge formed a different community in each reactor at 30 d HRT; however, no further significant microbial shift occurred at lower HRTs. The presence of specific hydrolytic and acidogenic Flavobacteriales and Clostriales in the acidogenic reactor may have allowed for enhanced hydrolysis and acidogenesis, leading to higher organic loading tolerance at 10 d HRT. Methanogenic activity in the acidogenic reactor may have been performed by Methanobacteriales and Methanosarcinaceae. Operation of the acidogenic reactor at neutral pH may have to be considered to ensure the cultivation of propionate oxidising bacteria, which could in turn, prevent reactor “souring” during high load conditions. |
| first_indexed | 2024-10-01T06:26:52Z |
| format | Journal Article |
| id | ntu-10356/99554 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2024-10-01T06:26:52Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | ntu-10356/995542020-09-26T22:01:08Z Comparison of single-stage and two-phase anaerobic sludge digestion systems : performance and microbial community dynamics Maspolim, Yogananda Zhou, Yan Guo, Chenghong Xiao, Keke Ng, Wun Jern School of Civil and Environmental Engineering Nanyang Environment and Water Research Institute DRNTU::Engineering::Environmental engineering::Water treatment This study compared reactor performance and the respective microbial community dynamics in the conventional single-stage and 2-phase anaerobic digestion (AD) systems, treating municipal sludge to generate methane. The 2-phase system’s COD and VS reduction, and methane production could be maintained throughout the three HRTs tested (p = 0.05), which was associated with an increase in organic loading (30 d = 1.5 g COD L−1 d−1, 20 d = 2.2 g COD L−1 d−1 and 10 d = 3.5 g COD L−1 d−1); but this was not so in the single-stage system where it deteriorated at HRT of 10 d (p = 0.05) due to impairment of particulate COD reduction. qPCR, DGGE and the subsequent phylogenetic analysis revealed that microbial adaptation occurred as the seed sludge formed a different community in each reactor at 30 d HRT; however, no further significant microbial shift occurred at lower HRTs. The presence of specific hydrolytic and acidogenic Flavobacteriales and Clostriales in the acidogenic reactor may have allowed for enhanced hydrolysis and acidogenesis, leading to higher organic loading tolerance at 10 d HRT. Methanogenic activity in the acidogenic reactor may have been performed by Methanobacteriales and Methanosarcinaceae. Operation of the acidogenic reactor at neutral pH may have to be considered to ensure the cultivation of propionate oxidising bacteria, which could in turn, prevent reactor “souring” during high load conditions. NRF (Natl Research Foundation, S’pore) Accepted version 2014-10-17T02:45:06Z 2019-12-06T20:08:45Z 2014-10-17T02:45:06Z 2019-12-06T20:08:45Z 2014 2014 Journal Article Maspolim, Y., Zhou, Y., Guo, C., Xiao, K., & Ng, W. J. (2014). Comparison of single-stage and two-phase anaerobic sludge digestion systems: Performance and microbial community dynamics. Chemosphere, 140, 54-62. 0045-6535 https://hdl.handle.net/10356/99554 http://hdl.handle.net/10220/24056 10.1016/j.chemosphere.2014.07.028 en Chemosphere © 2014 Elsevier Ltd. This is the author created version of a work that has been peer reviewed and accepted for publication by Chemosphere, Elsevier Ltd. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: [DOI:http://dx.doi.org/10.1016/j.chemosphere.2014.07.028]. 35 p. application/pdf |
| spellingShingle | DRNTU::Engineering::Environmental engineering::Water treatment Maspolim, Yogananda Zhou, Yan Guo, Chenghong Xiao, Keke Ng, Wun Jern Comparison of single-stage and two-phase anaerobic sludge digestion systems : performance and microbial community dynamics |
| title | Comparison of single-stage and two-phase anaerobic sludge digestion systems : performance and microbial community dynamics |
| title_full | Comparison of single-stage and two-phase anaerobic sludge digestion systems : performance and microbial community dynamics |
| title_fullStr | Comparison of single-stage and two-phase anaerobic sludge digestion systems : performance and microbial community dynamics |
| title_full_unstemmed | Comparison of single-stage and two-phase anaerobic sludge digestion systems : performance and microbial community dynamics |
| title_short | Comparison of single-stage and two-phase anaerobic sludge digestion systems : performance and microbial community dynamics |
| title_sort | comparison of single stage and two phase anaerobic sludge digestion systems performance and microbial community dynamics |
| topic | DRNTU::Engineering::Environmental engineering::Water treatment |
| url | https://hdl.handle.net/10356/99554 http://hdl.handle.net/10220/24056 |
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