Kinetin riboside preferentially induces apoptosis by modulating Bcl-2 family proteins and caspase-3 in cancer cells
Here, we demonstrate that kinetin riboside (KR), a cytokinin analog, induces apoptosis in HeLa and mouse melanoma B16F-10 cells. KR disrupted the mitochondrial membrane potential and induced the release of cytochrome c and activation of caspase-3. Bad were upregulated while Bcl-2 was down-regu...
| Main Authors: | , , , , , , , |
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| Format: | Journal Article |
| Language: | English |
| Published: |
2012
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| Subjects: | |
| Online Access: | https://hdl.handle.net/10356/99906 http://hdl.handle.net/10220/8384 |
| _version_ | 1824454231296311296 |
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| author | Choi, Bo-Hwa Kim, Wanil Wang, Qiuxia Chelsia Kim, Dong-Chan Tan, Swee Ngin Yong, Jean Wan Hong Kim, Kyong-Tai Yoon, Ho Sup |
| author2 | School of Biological Sciences |
| author_facet | School of Biological Sciences Choi, Bo-Hwa Kim, Wanil Wang, Qiuxia Chelsia Kim, Dong-Chan Tan, Swee Ngin Yong, Jean Wan Hong Kim, Kyong-Tai Yoon, Ho Sup |
| author_sort | Choi, Bo-Hwa |
| collection | NTU |
| description | Here, we demonstrate that kinetin riboside (KR), a cytokinin analog, induces apoptosis in HeLa and mouse melanoma
B16F-10 cells. KR disrupted the mitochondrial membrane potential and induced the release of cytochrome c and activation
of caspase-3. Bad were upregulated while Bcl-2 was down-regulated under KR exposure. A tumor growth in mice was
dramatically suppressed by KR. In contrast, human skin fibroblast CCL-116 and bovine primary fibroblast cells show
resistances to KR and no significant changes in Bad, Bcl-XL, and cleaved PARP were observed. Our data suggest that
KR selectively induces apoptosis in cancer cells through the classical mitochondria dependent apoptosis pathway. |
| first_indexed | 2025-02-19T03:19:01Z |
| format | Journal Article |
| id | ntu-10356/99906 |
| institution | Nanyang Technological University |
| language | English |
| last_indexed | 2025-02-19T03:19:01Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | ntu-10356/999062023-02-28T17:04:51Z Kinetin riboside preferentially induces apoptosis by modulating Bcl-2 family proteins and caspase-3 in cancer cells Choi, Bo-Hwa Kim, Wanil Wang, Qiuxia Chelsia Kim, Dong-Chan Tan, Swee Ngin Yong, Jean Wan Hong Kim, Kyong-Tai Yoon, Ho Sup School of Biological Sciences DRNTU::Science::Biological sciences Here, we demonstrate that kinetin riboside (KR), a cytokinin analog, induces apoptosis in HeLa and mouse melanoma B16F-10 cells. KR disrupted the mitochondrial membrane potential and induced the release of cytochrome c and activation of caspase-3. Bad were upregulated while Bcl-2 was down-regulated under KR exposure. A tumor growth in mice was dramatically suppressed by KR. In contrast, human skin fibroblast CCL-116 and bovine primary fibroblast cells show resistances to KR and no significant changes in Bad, Bcl-XL, and cleaved PARP were observed. Our data suggest that KR selectively induces apoptosis in cancer cells through the classical mitochondria dependent apoptosis pathway. Accepted version 2012-08-14T04:21:28Z 2019-12-06T20:13:22Z 2012-08-14T04:21:28Z 2019-12-06T20:13:22Z 2007 2007 Journal Article Choi, B. -H., Kim, W., Wang, Q. C., Kim, D., -C., Tan, S., N., Yong, J. W. H., et al. (2008). Kinetin riboside preferentially induces apoptosis by modulating Bcl-2 family proteins and caspase-3 in cancer cells. Cancer Letters, 261(1), 37-45. https://hdl.handle.net/10356/99906 http://hdl.handle.net/10220/8384 10.1016/j.canlet.2007.11.014 en Cancer letters © 2007 Elsevier. This is the author created version of a work that has been peer reviewed and accepted for publication by Cancer Letters, Elsevier. It incorporates referee’s comments but changes resulting from the publishing process, such as copyediting, structural formatting, may not be reflected in this document. The published version is available at: http://dx.doi.org/10.1016/j.canlet.2007.11.014. application/pdf |
| spellingShingle | DRNTU::Science::Biological sciences Choi, Bo-Hwa Kim, Wanil Wang, Qiuxia Chelsia Kim, Dong-Chan Tan, Swee Ngin Yong, Jean Wan Hong Kim, Kyong-Tai Yoon, Ho Sup Kinetin riboside preferentially induces apoptosis by modulating Bcl-2 family proteins and caspase-3 in cancer cells |
| title | Kinetin riboside preferentially induces apoptosis by modulating Bcl-2 family proteins and caspase-3 in cancer cells |
| title_full | Kinetin riboside preferentially induces apoptosis by modulating Bcl-2 family proteins and caspase-3 in cancer cells |
| title_fullStr | Kinetin riboside preferentially induces apoptosis by modulating Bcl-2 family proteins and caspase-3 in cancer cells |
| title_full_unstemmed | Kinetin riboside preferentially induces apoptosis by modulating Bcl-2 family proteins and caspase-3 in cancer cells |
| title_short | Kinetin riboside preferentially induces apoptosis by modulating Bcl-2 family proteins and caspase-3 in cancer cells |
| title_sort | kinetin riboside preferentially induces apoptosis by modulating bcl 2 family proteins and caspase 3 in cancer cells |
| topic | DRNTU::Science::Biological sciences |
| url | https://hdl.handle.net/10356/99906 http://hdl.handle.net/10220/8384 |
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