PERBANDINGAN EKSPRESI PROTEIN Bax dan APOPTOSIS SEL TROFOBLAS PLASENTA ANTARA PREEKLAMPSIA BERAT / EKLAMPSIA DENGAN KEHAMILAN NORMOTENSI
Background: Placenta is a central and important focus on the pathogenesis of preeclampsia. Failure of trophoblast cell to the spiral arteries remodeling process due to excessive apoptosis causes uteroplacenter ischaemia and damage of endothelial cells that give rise to clinical manifestations of pre...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2012
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| Summary: | Background: Placenta is a central and important focus on the
pathogenesis of preeclampsia. Failure of trophoblast cell to the spiral
arteries remodeling process due to excessive apoptosis causes
uteroplacenter ischaemia and damage of endothelial cells that give rise to
clinical manifestations of preeclampsia. Excessive throphoblast apoptosis
in preeclampsia mainly occurs through the intrinsic pathway in which
expression of Bax protein increases mitocondrial membranes permeability
of cytochrom C which further activate the caspase cascade and become
involved in the process of cell death.
Objective: To compare the expression of Bax protein and
throphoblastic apoptosis process between severe preeclampsia/eclampsia
and the normotensive pregnancy.
Methods: Cross sectional study which consist of 43 severe
preeclampsia/eclampsia pregnancies and 38 third trimester normotensive
pregnancies, recruited between October 2011 � March 2012. Trophoblastic
Bax protein expression is measured by imunohistochemical staining
technique and trophoblast apoptosis process is examined by the Tunel
assay. Statistical analysis using the independent t test (p<0.05).
Results: Bax protein expression was significantly higher in
trophoblast cell of severe preeclampsia/eclampsia compared to
normotensive pregnancy (1.7 vs 1.4, p=0.00). Bax expression positively
correlated (r=0.01) with mean arterial pressure which is increasing of the
mean arterial pressure will increase the expression of the Bax protein.
There was no significant difference in trophoblastic apoptosis index
between severe preeclampsia/eclampsia pregnancy and normotensive
pregnancy (23.8 vs 35.5, p= 0.10).
Conclusions: Bax protein expression was significantly higher in
severe preeclampsia/eclampsia than normotensive pregnancy. There was
no significantly difference in trophoblast apoptosis index between severe
preeclampsia/eclampsia and normotensive pregnancy. |
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