PENGGUNAAN BAKTERI PROTEOLITIK NON KOLAGENASE DARI LIMBAH INDUSTRI TUNA SEBAGAI AGENSIA BATING PADA PROSES PENYAMAKAN KULIT IKAN TUNA

The purpose of research is the application of protease enzyme from tuna industry waste as a bating agency in the tanning of tuna fish hides. The first stage of the research is to isolate proteolitic bacteria from the wash water of the intestine of the tuna fish loins at Bitung North Sulawesi. The se...

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Main Authors: , Ir. AGNES TRIASIH AGUSTIN MAPP.Sc, , Prof. Dr. Ir. Suharjono Triatmojo, MS.
Format: Thesis
Published: [Yogyakarta] : Universitas Gadjah Mada 2012
Subjects:
ETD
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author , Ir. AGNES TRIASIH AGUSTIN MAPP.Sc
, Prof. Dr. Ir. Suharjono Triatmojo, MS.
author_facet , Ir. AGNES TRIASIH AGUSTIN MAPP.Sc
, Prof. Dr. Ir. Suharjono Triatmojo, MS.
author_sort , Ir. AGNES TRIASIH AGUSTIN MAPP.Sc
collection UGM
description The purpose of research is the application of protease enzyme from tuna industry waste as a bating agency in the tanning of tuna fish hides. The first stage of the research is to isolate proteolitic bacteria from the wash water of the intestine of the tuna fish loins at Bitung North Sulawesi. The second stage of the research was the fermentation of the proteolitic bacteria in the activated on pH and different temperature. The third stage is the application of the produced protease enzyme as a bating agency in the tanning of tuna fish hides. Of the 10 isolates produced from the waste water of the tuna fish intestines are the Bacillus sp. Sequencing of 16 S gen rRNA has homology as Bacillus subtilis with identity more 97 %. The protease content is achieved after 20 hours. The maximum adaptation produced will have the highest activity at 370 C is (5.30U/ml) and 15U/ml at pH 6,0. The treatment with protease produce by bacillus sp of 1%, 1.5% and 2% for bating agency comparable with commercial enzyme of 1% not significantly different there are for strength, flexibility, stretch. Therefore 1.5% protease of Bacillus sp already meets the standard requirements of SNI 06-0277-1998.
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format Thesis
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institution Universiti Gadjah Mada
last_indexed 2024-03-13T22:42:18Z
publishDate 2012
publisher [Yogyakarta] : Universitas Gadjah Mada
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spelling oai:generic.eprints.org:1003772016-03-04T08:47:12Z https://repository.ugm.ac.id/100377/ PENGGUNAAN BAKTERI PROTEOLITIK NON KOLAGENASE DARI LIMBAH INDUSTRI TUNA SEBAGAI AGENSIA BATING PADA PROSES PENYAMAKAN KULIT IKAN TUNA , Ir. AGNES TRIASIH AGUSTIN MAPP.Sc , Prof. Dr. Ir. Suharjono Triatmojo, MS. ETD The purpose of research is the application of protease enzyme from tuna industry waste as a bating agency in the tanning of tuna fish hides. The first stage of the research is to isolate proteolitic bacteria from the wash water of the intestine of the tuna fish loins at Bitung North Sulawesi. The second stage of the research was the fermentation of the proteolitic bacteria in the activated on pH and different temperature. The third stage is the application of the produced protease enzyme as a bating agency in the tanning of tuna fish hides. Of the 10 isolates produced from the waste water of the tuna fish intestines are the Bacillus sp. Sequencing of 16 S gen rRNA has homology as Bacillus subtilis with identity more 97 %. The protease content is achieved after 20 hours. The maximum adaptation produced will have the highest activity at 370 C is (5.30U/ml) and 15U/ml at pH 6,0. The treatment with protease produce by bacillus sp of 1%, 1.5% and 2% for bating agency comparable with commercial enzyme of 1% not significantly different there are for strength, flexibility, stretch. Therefore 1.5% protease of Bacillus sp already meets the standard requirements of SNI 06-0277-1998. [Yogyakarta] : Universitas Gadjah Mada 2012 Thesis NonPeerReviewed , Ir. AGNES TRIASIH AGUSTIN MAPP.Sc and , Prof. Dr. Ir. Suharjono Triatmojo, MS. (2012) PENGGUNAAN BAKTERI PROTEOLITIK NON KOLAGENASE DARI LIMBAH INDUSTRI TUNA SEBAGAI AGENSIA BATING PADA PROSES PENYAMAKAN KULIT IKAN TUNA. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=56997
spellingShingle ETD
, Ir. AGNES TRIASIH AGUSTIN MAPP.Sc
, Prof. Dr. Ir. Suharjono Triatmojo, MS.
PENGGUNAAN BAKTERI PROTEOLITIK NON KOLAGENASE DARI LIMBAH INDUSTRI TUNA SEBAGAI AGENSIA BATING PADA PROSES PENYAMAKAN KULIT IKAN TUNA
title PENGGUNAAN BAKTERI PROTEOLITIK NON KOLAGENASE DARI LIMBAH INDUSTRI TUNA SEBAGAI AGENSIA BATING PADA PROSES PENYAMAKAN KULIT IKAN TUNA
title_full PENGGUNAAN BAKTERI PROTEOLITIK NON KOLAGENASE DARI LIMBAH INDUSTRI TUNA SEBAGAI AGENSIA BATING PADA PROSES PENYAMAKAN KULIT IKAN TUNA
title_fullStr PENGGUNAAN BAKTERI PROTEOLITIK NON KOLAGENASE DARI LIMBAH INDUSTRI TUNA SEBAGAI AGENSIA BATING PADA PROSES PENYAMAKAN KULIT IKAN TUNA
title_full_unstemmed PENGGUNAAN BAKTERI PROTEOLITIK NON KOLAGENASE DARI LIMBAH INDUSTRI TUNA SEBAGAI AGENSIA BATING PADA PROSES PENYAMAKAN KULIT IKAN TUNA
title_short PENGGUNAAN BAKTERI PROTEOLITIK NON KOLAGENASE DARI LIMBAH INDUSTRI TUNA SEBAGAI AGENSIA BATING PADA PROSES PENYAMAKAN KULIT IKAN TUNA
title_sort penggunaan bakteri proteolitik non kolagenase dari limbah industri tuna sebagai agensia bating pada proses penyamakan kulit ikan tuna
topic ETD
work_keys_str_mv AT iragnestriasihagustinmappsc penggunaanbakteriproteolitiknonkolagenasedarilimbahindustritunasebagaiagensiabatingpadaprosespenyamakankulitikantuna
AT profdrirsuharjonotriatmojoms penggunaanbakteriproteolitiknonkolagenasedarilimbahindustritunasebagaiagensiabatingpadaprosespenyamakankulitikantuna