| Summary: | Curcumin, a natural polyphenolic compound with an anticancer properties
presents in the turmeric (Curcuma longa L.). The optimum potensial for cancer
therapy is limited by its poor oral biovability. The objectives of this study were to
improve bioavability of curcumin by preparing curcumin nanoparticles using
chitosan and tripolyphosphat at various concentrations, to characterize, to evaluate
the stability of curcumin nanoparticles in artificial gastric fluid (AGF) and artificial
intestinal fluid (AIF), to identify the cytotoxicity effect of curcumin nanoparticles
against HeLa cell lines and normal cell lines (Vero) and to investigate the cellular
uptake of curcumin nanoparticles.
In this study, curcumin nanoparticles prepared by ionic gelation method using
chitosan and TPP at various concentrations. The curcumin nanoparticles were then
characterized for their physicochemical characterizations include particle size, zeta
potensial morphology and entrapment efficiency. Furthermore, curcumin-chitosan
nanoparticles were evaluated for their stability in artificial gastric fluid (AGF) and
artificial intestinal fluid (AIF) by quantitating the release of curcumin from the
nanoparticles as a function of time, cellular uptake of curcumin nanoparticles
investigated using fluorescence microscopy and cytotoxic effect against HeLa cell
lines and normal cell lines (Vero) by calculating the level of LC50 which was based
on the percentage of the cell death following the 24 hours incubation with the
nanoparticles.
It was demonstrated that curcumin nanoparticles were prepared by ionic
gelation technique using low viscous chitosan and TPP at various concentrations.
The mean particle size of curcumin nanoparticles were 113,9 to 341, 2 nm, zeta
potensial were (-)2,57 to (+)27,55, entrapment efficiency of curcumin in
nanoparticles were 73,63 to 85,71% with the observed shapes of nanoparticles was
spherical. Curcumin nanoparticles were stable in artificial gastric fluid and artificial
intestinal fluid. Curcumin nanoparticles were more cytotoxic to HeLa cell line and
there was a statistically significant difference in potensial cytotoxicity in HeLa cell
lines in comparison to vero cell lines, cellular uptake studies of curcumin
nanoparticles showed that nanoparticles were able to intenalize the HeLa cell lines.
|
|---|