Preparasi Nano Partikel Gamavuton-0 Menggunakan Kitosan Rantai Sedang Dan Tripolifosfat Serta Uji Cellular Uptake Pada Kultursel Mcf-7 Secara In Vitro
Gamavuton-0 (GVT-0) or [1,5-bis(4�-hidroxy-3�-metoxyphenyl)-1,4-pentadiene-3-on] is one of analogue compounds of curcumin. To obtain the optimum therapeutic effect, GVT-0 has drawback is that the lack of solubility in water that affecting its oral bioavailability. The aim of this research was to...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2012
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| author | , RIRIN , Dr. rer. nat. Ronny Martien, M.Si., |
| author_facet | , RIRIN , Dr. rer. nat. Ronny Martien, M.Si., |
| author_sort | , RIRIN |
| collection | UGM |
| description | Gamavuton-0 (GVT-0) or [1,5-bis(4�-hidroxy-3�-metoxyphenyl)-1,4-pentadiene-3-on] is one of analogue compounds of curcumin. To obtain the optimum therapeutic effect, GVT-0 has drawback is that the lack of solubility in water that affecting its oral bioavailability. The aim of this research was to find out the stability of chitosan-loaded GVT-0 in dissolution medium and the ability to enter into MCF-7 cell line.
Preliminary research carried out by purity test through TLC and melting point. GVT-0 preparation was to improve the delivery in the form of nanoparticles in which chitosan medium chain as polymer and tripolyphosphate as cross-linker. Nanoparticle of chitosan-loaded GVT-0 characterization included morphology, distribution and particle size, zeta potential, and entrapment efficiency. Stability test carried out in HCl 0,1 N and artificial intestinal fluid (AIF), whereas cellular uptake assay done by MCF-7 cell line as the model cell.
Results of this research showed that GVT-0 being used was pure by TLC and melting point which were indicated by one single spot in UV 254 and 366 nm and not more than 2°C rises in melting point, chitosan-loaded GVT-0 nanoparticles could be made by ionotropic gelation. The obtained nanoparticles was spherical in shape. The particle size in the range of 30.0-282.2 nm with the polydispersity index ranged from 1.3-10.5, zeta potential were +5.5 upto +11.8 mV. The entrapment efficiency ranged from 49.73 to 58.99%. In vitro stability test showed that nanoparticles stability were pH dependent. The nanoparticles relative unstable in both medium 0.1 N HCl and artificial intestinal fluid. In vitro cellular uptake assay showed that nanoparticle chitosan-loaded GVT-0 were able to enter the MCF-7 cell line. Those results above indicated that nanoparticle may be used to deliver GVT-0 effectively. |
| first_indexed | 2024-03-13T22:44:14Z |
| format | Thesis |
| id | oai:generic.eprints.org:100906 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:44:14Z |
| publishDate | 2012 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1009062016-03-04T08:48:24Z https://repository.ugm.ac.id/100906/ Preparasi Nano Partikel Gamavuton-0 Menggunakan Kitosan Rantai Sedang Dan Tripolifosfat Serta Uji Cellular Uptake Pada Kultursel Mcf-7 Secara In Vitro , RIRIN , Dr. rer. nat. Ronny Martien, M.Si., ETD Gamavuton-0 (GVT-0) or [1,5-bis(4�-hidroxy-3�-metoxyphenyl)-1,4-pentadiene-3-on] is one of analogue compounds of curcumin. To obtain the optimum therapeutic effect, GVT-0 has drawback is that the lack of solubility in water that affecting its oral bioavailability. The aim of this research was to find out the stability of chitosan-loaded GVT-0 in dissolution medium and the ability to enter into MCF-7 cell line. Preliminary research carried out by purity test through TLC and melting point. GVT-0 preparation was to improve the delivery in the form of nanoparticles in which chitosan medium chain as polymer and tripolyphosphate as cross-linker. Nanoparticle of chitosan-loaded GVT-0 characterization included morphology, distribution and particle size, zeta potential, and entrapment efficiency. Stability test carried out in HCl 0,1 N and artificial intestinal fluid (AIF), whereas cellular uptake assay done by MCF-7 cell line as the model cell. Results of this research showed that GVT-0 being used was pure by TLC and melting point which were indicated by one single spot in UV 254 and 366 nm and not more than 2°C rises in melting point, chitosan-loaded GVT-0 nanoparticles could be made by ionotropic gelation. The obtained nanoparticles was spherical in shape. The particle size in the range of 30.0-282.2 nm with the polydispersity index ranged from 1.3-10.5, zeta potential were +5.5 upto +11.8 mV. The entrapment efficiency ranged from 49.73 to 58.99%. In vitro stability test showed that nanoparticles stability were pH dependent. The nanoparticles relative unstable in both medium 0.1 N HCl and artificial intestinal fluid. In vitro cellular uptake assay showed that nanoparticle chitosan-loaded GVT-0 were able to enter the MCF-7 cell line. Those results above indicated that nanoparticle may be used to deliver GVT-0 effectively. [Yogyakarta] : Universitas Gadjah Mada 2012 Thesis NonPeerReviewed , RIRIN and , Dr. rer. nat. Ronny Martien, M.Si., (2012) Preparasi Nano Partikel Gamavuton-0 Menggunakan Kitosan Rantai Sedang Dan Tripolifosfat Serta Uji Cellular Uptake Pada Kultursel Mcf-7 Secara In Vitro. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=57536 |
| spellingShingle | ETD , RIRIN , Dr. rer. nat. Ronny Martien, M.Si., Preparasi Nano Partikel Gamavuton-0 Menggunakan Kitosan Rantai Sedang Dan Tripolifosfat Serta Uji Cellular Uptake Pada Kultursel Mcf-7 Secara In Vitro |
| title | Preparasi Nano Partikel Gamavuton-0 Menggunakan Kitosan Rantai Sedang Dan Tripolifosfat Serta Uji Cellular Uptake Pada Kultursel Mcf-7
Secara In Vitro |
| title_full | Preparasi Nano Partikel Gamavuton-0 Menggunakan Kitosan Rantai Sedang Dan Tripolifosfat Serta Uji Cellular Uptake Pada Kultursel Mcf-7
Secara In Vitro |
| title_fullStr | Preparasi Nano Partikel Gamavuton-0 Menggunakan Kitosan Rantai Sedang Dan Tripolifosfat Serta Uji Cellular Uptake Pada Kultursel Mcf-7
Secara In Vitro |
| title_full_unstemmed | Preparasi Nano Partikel Gamavuton-0 Menggunakan Kitosan Rantai Sedang Dan Tripolifosfat Serta Uji Cellular Uptake Pada Kultursel Mcf-7
Secara In Vitro |
| title_short | Preparasi Nano Partikel Gamavuton-0 Menggunakan Kitosan Rantai Sedang Dan Tripolifosfat Serta Uji Cellular Uptake Pada Kultursel Mcf-7
Secara In Vitro |
| title_sort | preparasi nano partikel gamavuton 0 menggunakan kitosan rantai sedang dan tripolifosfat serta uji cellular uptake pada kultursel mcf 7 secara in vitro |
| topic | ETD |
| work_keys_str_mv | AT ririn preparasinanopartikelgamavuton0menggunakankitosanrantaisedangdantripolifosfatsertaujicellularuptakepadakulturselmcf7secarainvitro AT drrernatronnymartienmsi preparasinanopartikelgamavuton0menggunakankitosanrantaisedangdantripolifosfatsertaujicellularuptakepadakulturselmcf7secarainvitro |