| Summary: | Maize is used as a raw material for sekelan processing, the staple food in
rural communities at Sigedong, Tretep district, Temanggung, Central Java. In general,
maize harvested in the village on the rainy season so the chances of contamination of
mycotoxin-producing fungi is high.
Based on the ecological, Fusarium suspected as a highest polluters fungus,
therefore it is necessary to know the prevalence of the Fusarium fungus
contamination and toxins produced both on maize as a raw material and sekelan. In
addition, it is necessary to evaluate Fumonisin exposure in the population by using
urine.
Enumeration, isolation and identification of the Fusarium fungus done
macroscopically and microscopically. Analysis presence of Fumonisin B1 (FB1) in
maize and sekelan using IAC-HPLC-FLD and urine analysis using LC-MS/MS.
The results showed that the Fusarium fungus contamination in maize,
respectively dominated by Fusarium verticillioides, Fusarium proliferatum, Fusarium
sporotrichioides, Fusarium solani, Fusarium oxysporum, Fusarium subglutinans and
Fusarium graminearum, whereas Fusarium not found in sekelan but other genera are
Aspergillus, Mucor, Penicillium, and Rhizopus founded. Effectively, inside the
process of sekelan especially dehulling and soaking are capable to reduce 77%
Fumonisin B1 and Fumonisin B1 exposure to humans was detected in urine of elderly
respondents indicating the exposure of Fumonisin B1 in their diet.
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