Summary: | We developed hydroxyapatite (HA)
granules made from local raw materials (GranuMaS™),
which have been fabricated using a novel method
(Patent (Pending) No. PI 2004 0748). GranuMaS™
have been fully characterized using X-ray diffraction,
scanning electron microscopy, energy dispersive x-ray,
Fourier transform infra-red spectroscopy and
inductively coupled plasma methods. It has also been
shown to comply with the ASTM F1185 - 88 (1993)
specification standards.
Biocompatibility studies have been performed on the
product, which include neutral red cytotoxicity assay
using cultured fibroblast and CRL-1427 osteoblast cells;
MTT (Tetrazolium Salt) assay; cytotoxicity studies for
apoptosis using Acridine Orange / Propidium Iodide
(AO/PI) dual staining of V79 cells in DMEM; and
COMET assay genotoxicity studies using L-929
(normal mouse epithelial) cells.
An in vivo implantation study involving New Zealand
White rabbits were carried out in the course of this
study.
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