Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice
Light microscopic (LM) and transmission electron microscopic (TEM) observations were performed in the kidneys of oral contraceptive treated mice. Experimental groups were given 0.1 ml of olive oil containing 0.015 fraction of (Neogynon) tablet daily. Control groups were give olive oil alone. Five e...
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| Format: | Article |
| Language: | English |
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Department of Anatomy, National University of Singapore
2009
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| Online Access: | http://irep.iium.edu.my/10689/1/Effectof_Contraciptive_pills.pdf |
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| author | Al-Ani, Imad Matloub Dally Noor Al-Deen, Jinan A. Kashmola, Muhanad Ali Hamdon |
| author_facet | Al-Ani, Imad Matloub Dally Noor Al-Deen, Jinan A. Kashmola, Muhanad Ali Hamdon |
| author_sort | Al-Ani, Imad Matloub Dally |
| collection | IIUM |
| description | Light microscopic (LM) and transmission electron microscopic (TEM) observations were performed in the kidneys of oral contraceptive treated mice. Experimental groups were given 0.1 ml of olive oil containing 0.015 fraction of (Neogynon) tablet daily. Control groups were
give olive oil alone. Five experimental and three control animals were scarifi ed after 1, 3, 6, 9, and 12 estrus cycles. Glomerulosclerosis, hyaline arteriopathy associated with focal areas of interstitial fi brosis were observed in the fi rst cycle. Mild hypertrophy of juxtaglomerular apparatus “JGA” appeared in the 3rd cycle with increased granularity of the JGCs. Proliferative-sclerosing
stages of focal glomerulonephritis were observed and were more advanced in cycle twelve. By transmission electron microscope, there were slight changes in the glomeruli during cycle one. These evident gradually developed in cycle three, thus the glomeruli showed disordered capillary
loop occlusion. In cycle six, capillary lumens were occluded by protuberance projection. There were hyperactivity and proliferation of mesangial cells and electron-dense material deposits in glomerular basal lamina; these deposits were accompanied with electron-lucent materials appeared in cycle 9 behind swelled mesangial cells indicating hyperactivity and proliferative cells which showed abundant RER and mitochondria. These lesions were correlated with hyper-granularity of JGCs and hypertrophied JGA. The JGGs appeared in cycle 12 as heterogenized size. These changes indicate that abuse of oral contraceptives is relatively unsafe, especially after long-term of high-dose usage. |
| first_indexed | 2024-03-05T22:43:56Z |
| format | Article |
| id | oai:generic.eprints.org:10689 |
| institution | International Islamic University Malaysia |
| language | English |
| last_indexed | 2024-03-05T22:43:56Z |
| publishDate | 2009 |
| publisher | Department of Anatomy, National University of Singapore |
| record_format | dspace |
| spelling | oai:generic.eprints.org:106892015-12-18T03:24:27Z http://irep.iium.edu.my/10689/ Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice Al-Ani, Imad Matloub Dally Noor Al-Deen, Jinan A. Kashmola, Muhanad Ali Hamdon QM Human anatomy QP Physiology RB Pathology Light microscopic (LM) and transmission electron microscopic (TEM) observations were performed in the kidneys of oral contraceptive treated mice. Experimental groups were given 0.1 ml of olive oil containing 0.015 fraction of (Neogynon) tablet daily. Control groups were give olive oil alone. Five experimental and three control animals were scarifi ed after 1, 3, 6, 9, and 12 estrus cycles. Glomerulosclerosis, hyaline arteriopathy associated with focal areas of interstitial fi brosis were observed in the fi rst cycle. Mild hypertrophy of juxtaglomerular apparatus “JGA” appeared in the 3rd cycle with increased granularity of the JGCs. Proliferative-sclerosing stages of focal glomerulonephritis were observed and were more advanced in cycle twelve. By transmission electron microscope, there were slight changes in the glomeruli during cycle one. These evident gradually developed in cycle three, thus the glomeruli showed disordered capillary loop occlusion. In cycle six, capillary lumens were occluded by protuberance projection. There were hyperactivity and proliferation of mesangial cells and electron-dense material deposits in glomerular basal lamina; these deposits were accompanied with electron-lucent materials appeared in cycle 9 behind swelled mesangial cells indicating hyperactivity and proliferative cells which showed abundant RER and mitochondria. These lesions were correlated with hyper-granularity of JGCs and hypertrophied JGA. The JGGs appeared in cycle 12 as heterogenized size. These changes indicate that abuse of oral contraceptives is relatively unsafe, especially after long-term of high-dose usage. Department of Anatomy, National University of Singapore 2009 Article PeerReviewed application/pdf en http://irep.iium.edu.my/10689/1/Effectof_Contraciptive_pills.pdf Al-Ani, Imad Matloub Dally and Noor Al-Deen, Jinan A. and Kashmola, Muhanad Ali Hamdon (2009) Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice. Annals of Microscopy, 9. pp. 63-72. ISSN 0219-2209 http://www.microscopy.org.sg/journal.htm |
| spellingShingle | QM Human anatomy QP Physiology RB Pathology Al-Ani, Imad Matloub Dally Noor Al-Deen, Jinan A. Kashmola, Muhanad Ali Hamdon Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice |
| title | Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice |
| title_full | Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice |
| title_fullStr | Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice |
| title_full_unstemmed | Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice |
| title_short | Light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice |
| title_sort | light and transmission electron microscopic study on the effect of contraceptive pills on the glomerulus and juxtaglomerular apparatus in mice |
| topic | QM Human anatomy QP Physiology RB Pathology |
| url | http://irep.iium.edu.my/10689/1/Effectof_Contraciptive_pills.pdf |
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