ANALISIS DNA BABI PADA BURGER MENGGUNAKAN REAL-TIME PCR (Polymerase Chain reaction)
Halal issues related to contamination of pork is one thing that has always been a major concern and consideration in the food industry because of religion, health and economy reasons. Real-time PCR using primers specific species of ND5 mitochondrial DNA have conducted to identify the presence of por...
| Main Authors: | , |
|---|---|
| Format: | Thesis |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
|
| Subjects: |
| _version_ | 1826046106839023616 |
|---|---|
| author | , RENY SYAHRUNI , Prof. Sismindari, SU., Ph.D., Apt |
| author_facet | , RENY SYAHRUNI , Prof. Sismindari, SU., Ph.D., Apt |
| author_sort | , RENY SYAHRUNI |
| collection | UGM |
| description | Halal issues related to contamination of pork is one thing that has always
been a major concern and consideration in the food industry because of religion,
health and economy reasons. Real-time PCR using primers specific species of
ND5 mitochondrial DNA have conducted to identify the presence of porcine in
processed meat like burger that mixed meat of pork and beef or chicken that
combined with hot start method in fluoresence dye have been conducted to
identify the presence of porcine in processed meat like burger. Various
concentrations of pork added to the manufacture of beef and chicken burger from
the range 0.5% -100%. The results showed that the contamination can be detected
in all of pork samples that generate fragments of 227 bp amplification of pig
mitochondrial ND5 gene. Detection limit of porcine was 1 pg and 0,5% in burger
mixed with pork. |
| first_indexed | 2024-03-13T22:48:01Z |
| format | Thesis |
| id | oai:generic.eprints.org:118903 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:48:01Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1189032016-03-04T08:42:14Z https://repository.ugm.ac.id/118903/ ANALISIS DNA BABI PADA BURGER MENGGUNAKAN REAL-TIME PCR (Polymerase Chain reaction) , RENY SYAHRUNI , Prof. Sismindari, SU., Ph.D., Apt ETD Halal issues related to contamination of pork is one thing that has always been a major concern and consideration in the food industry because of religion, health and economy reasons. Real-time PCR using primers specific species of ND5 mitochondrial DNA have conducted to identify the presence of porcine in processed meat like burger that mixed meat of pork and beef or chicken that combined with hot start method in fluoresence dye have been conducted to identify the presence of porcine in processed meat like burger. Various concentrations of pork added to the manufacture of beef and chicken burger from the range 0.5% -100%. The results showed that the contamination can be detected in all of pork samples that generate fragments of 227 bp amplification of pig mitochondrial ND5 gene. Detection limit of porcine was 1 pg and 0,5% in burger mixed with pork. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , RENY SYAHRUNI and , Prof. Sismindari, SU., Ph.D., Apt (2013) ANALISIS DNA BABI PADA BURGER MENGGUNAKAN REAL-TIME PCR (Polymerase Chain reaction). UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=58881 |
| spellingShingle | ETD , RENY SYAHRUNI , Prof. Sismindari, SU., Ph.D., Apt ANALISIS DNA BABI PADA BURGER MENGGUNAKAN REAL-TIME PCR (Polymerase Chain reaction) |
| title | ANALISIS DNA BABI PADA BURGER MENGGUNAKAN
REAL-TIME PCR (Polymerase Chain reaction) |
| title_full | ANALISIS DNA BABI PADA BURGER MENGGUNAKAN
REAL-TIME PCR (Polymerase Chain reaction) |
| title_fullStr | ANALISIS DNA BABI PADA BURGER MENGGUNAKAN
REAL-TIME PCR (Polymerase Chain reaction) |
| title_full_unstemmed | ANALISIS DNA BABI PADA BURGER MENGGUNAKAN
REAL-TIME PCR (Polymerase Chain reaction) |
| title_short | ANALISIS DNA BABI PADA BURGER MENGGUNAKAN
REAL-TIME PCR (Polymerase Chain reaction) |
| title_sort | analisis dna babi pada burger menggunakan real time pcr polymerase chain reaction |
| topic | ETD |
| work_keys_str_mv | AT renysyahruni analisisdnababipadaburgermenggunakanrealtimepcrpolymerasechainreaction AT profsismindarisuphdapt analisisdnababipadaburgermenggunakanrealtimepcrpolymerasechainreaction |