Aktivitas Senyawa Semisintetik Kuasinoid dari Buah Makasar (Brucea javanica [L.] Merr) sebagai Antikanker dengan Target Protein P53, Bcl-2, Kaspase-3, COX-2 dan c-Myc
The Incidence of cervical cancer recently had a leading caused of cancer in woman mostly in developing country including Indonesia. Therefore the pursuit of that cancer drugs is still needed. Trial in vitro models of cervical cancer by using HeLa cell has been approved. It has been known the role of...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| author | , Sonlimar Mangunsong, Drs.,Apt.,M.Kes. , Prof. dr. Iwan Dwiprahasto, M.MedSc. |
| author_facet | , Sonlimar Mangunsong, Drs.,Apt.,M.Kes. , Prof. dr. Iwan Dwiprahasto, M.MedSc. |
| author_sort | , Sonlimar Mangunsong, Drs.,Apt.,M.Kes. |
| collection | UGM |
| description | The Incidence of cervical cancer recently had a leading caused of cancer in woman mostly
in developing country including Indonesia. Therefore the pursuit of that cancer drugs is still
needed. Trial in vitro models of cervical cancer by using HeLa cell has been approved. It has
been known the role of several gens in cancer development which include P53, Bcl-2, c-Myc and
Caspase-3. Due to apoptosis DNA repaired was influenced by P53 proteins complex. Cancer
development also depends on inflammatory effect contributed by COX-2. Brucea javanica (L.)
Merr as a herbal plant has been used to treat several diseases as antiinflamatory, antibabesial,
antivirus, antiplasmodial as well as anticancer. In this study it was performed how to isolate
bruceine A from the seed of �buah makasar�. Following had semi-synthesis of that compound
with each of chlorobenzoyl chloride, and benzoyl chloride. As a result bruceine A have been
isolated (0.0035%), 3-O-chlorobenzoylbruceine (38%) and 3-O-benzoyl bruceine (40%). The
cytotoxic activity and its toxicity were done both in HeLa and Vero cells. That isolated and
semisynthesis products confirmed by structural elucidation with UV/vis, FT-IR, H-NMR, CNMR,
HPLC, HRESI-MS and LC-MS. Molecular activity was tested by flow cytometry and
immunocytochemistry assay through induction apoptosis by increasing P53 and caspase-3 levels,
antiproliferation through the increasing of P53 expression and the lowering levels among of Bcl-2,
c-Myc, as well as COX-2. The IC50 value of bruceine A on HeLa cells as cytotoxicity effect were
87.45±13.0 μg/mL |
| first_indexed | 2024-03-13T22:48:17Z |
| format | Thesis |
| id | oai:generic.eprints.org:118988 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:48:17Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1189882016-03-04T08:43:37Z https://repository.ugm.ac.id/118988/ Aktivitas Senyawa Semisintetik Kuasinoid dari Buah Makasar (Brucea javanica [L.] Merr) sebagai Antikanker dengan Target Protein P53, Bcl-2, Kaspase-3, COX-2 dan c-Myc , Sonlimar Mangunsong, Drs.,Apt.,M.Kes. , Prof. dr. Iwan Dwiprahasto, M.MedSc. ETD The Incidence of cervical cancer recently had a leading caused of cancer in woman mostly in developing country including Indonesia. Therefore the pursuit of that cancer drugs is still needed. Trial in vitro models of cervical cancer by using HeLa cell has been approved. It has been known the role of several gens in cancer development which include P53, Bcl-2, c-Myc and Caspase-3. Due to apoptosis DNA repaired was influenced by P53 proteins complex. Cancer development also depends on inflammatory effect contributed by COX-2. Brucea javanica (L.) Merr as a herbal plant has been used to treat several diseases as antiinflamatory, antibabesial, antivirus, antiplasmodial as well as anticancer. In this study it was performed how to isolate bruceine A from the seed of �buah makasar�. Following had semi-synthesis of that compound with each of chlorobenzoyl chloride, and benzoyl chloride. As a result bruceine A have been isolated (0.0035%), 3-O-chlorobenzoylbruceine (38%) and 3-O-benzoyl bruceine (40%). The cytotoxic activity and its toxicity were done both in HeLa and Vero cells. That isolated and semisynthesis products confirmed by structural elucidation with UV/vis, FT-IR, H-NMR, CNMR, HPLC, HRESI-MS and LC-MS. Molecular activity was tested by flow cytometry and immunocytochemistry assay through induction apoptosis by increasing P53 and caspase-3 levels, antiproliferation through the increasing of P53 expression and the lowering levels among of Bcl-2, c-Myc, as well as COX-2. The IC50 value of bruceine A on HeLa cells as cytotoxicity effect were 87.45±13.0 μg/mL [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , Sonlimar Mangunsong, Drs.,Apt.,M.Kes. and , Prof. dr. Iwan Dwiprahasto, M.MedSc. (2013) Aktivitas Senyawa Semisintetik Kuasinoid dari Buah Makasar (Brucea javanica [L.] Merr) sebagai Antikanker dengan Target Protein P53, Bcl-2, Kaspase-3, COX-2 dan c-Myc. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=58967 |
| spellingShingle | ETD , Sonlimar Mangunsong, Drs.,Apt.,M.Kes. , Prof. dr. Iwan Dwiprahasto, M.MedSc. Aktivitas Senyawa Semisintetik Kuasinoid dari Buah Makasar (Brucea javanica [L.] Merr) sebagai Antikanker dengan Target Protein P53, Bcl-2, Kaspase-3, COX-2 dan c-Myc |
| title | Aktivitas Senyawa Semisintetik Kuasinoid dari Buah Makasar
(Brucea javanica [L.] Merr) sebagai Antikanker dengan Target
Protein P53, Bcl-2, Kaspase-3, COX-2 dan c-Myc |
| title_full | Aktivitas Senyawa Semisintetik Kuasinoid dari Buah Makasar
(Brucea javanica [L.] Merr) sebagai Antikanker dengan Target
Protein P53, Bcl-2, Kaspase-3, COX-2 dan c-Myc |
| title_fullStr | Aktivitas Senyawa Semisintetik Kuasinoid dari Buah Makasar
(Brucea javanica [L.] Merr) sebagai Antikanker dengan Target
Protein P53, Bcl-2, Kaspase-3, COX-2 dan c-Myc |
| title_full_unstemmed | Aktivitas Senyawa Semisintetik Kuasinoid dari Buah Makasar
(Brucea javanica [L.] Merr) sebagai Antikanker dengan Target
Protein P53, Bcl-2, Kaspase-3, COX-2 dan c-Myc |
| title_short | Aktivitas Senyawa Semisintetik Kuasinoid dari Buah Makasar
(Brucea javanica [L.] Merr) sebagai Antikanker dengan Target
Protein P53, Bcl-2, Kaspase-3, COX-2 dan c-Myc |
| title_sort | aktivitas senyawa semisintetik kuasinoid dari buah makasar brucea javanica l merr sebagai antikanker dengan target protein p53 bcl 2 kaspase 3 cox 2 dan c myc |
| topic | ETD |
| work_keys_str_mv | AT sonlimarmangunsongdrsaptmkes aktivitassenyawasemisintetikkuasinoiddaribuahmakasarbruceajavanicalmerrsebagaiantikankerdengantargetproteinp53bcl2kaspase3cox2dancmyc AT profdriwandwiprahastommedsc aktivitassenyawasemisintetikkuasinoiddaribuahmakasarbruceajavanicalmerrsebagaiantikankerdengantargetproteinp53bcl2kaspase3cox2dancmyc |