AKTIVITAS ANTIKANKER ALFA TERPINEOL DARI GETAH Pinus merkusii (Jungh. Et de Vriese) PADA SIKLUS SEL, APOPTOSIS DAN EKSPRESI PROTEIN (Bcl-2 dan Bax) SEL LINI MCF-7
Background: Previous study showed that alfa terpineol from Pinus merkusii had cytotoxic effet on several cancer cell line namely T47D and HeLa. Objective: The aim of this study was to determine the effect of alfa terpineol on cytotoxicity, cell cycle changes, apoptotic and the changes expression of...
| Main Authors: | , |
|---|---|
| Format: | Thesis |
| Published: |
[Yogyakarta] : Universitas Gadjah Mada
2013
|
| Subjects: |
| _version_ | 1826046168739610624 |
|---|---|
| author | , Damiana Sapta Candrasari , Prof.dr. Sofia Mubarika Haryana |
| author_facet | , Damiana Sapta Candrasari , Prof.dr. Sofia Mubarika Haryana |
| author_sort | , Damiana Sapta Candrasari |
| collection | UGM |
| description | Background: Previous study showed that alfa terpineol from Pinus merkusii had cytotoxic effet on several cancer cell line namely T47D and HeLa.
Objective: The aim of this study was to determine the effect of alfa terpineol on cytotoxicity, cell cycle changes, apoptotic and the changes expression of Bcl-2 and Bax in MCF-7 cell
Methods: The cytotoxicity activity of alfa terpineol was determined using MTT assay, where the IC50 value was used as a parameter for cytotoxicity. Cell cycle changes was detected by Pi staining and flowcytometri. Annexin V-Pi staining and flowcytometry was used for detecting early apoptotic cell. The expression of Bcl-2 and Bax protein was explored by immunocytochemistry. Result: MTT assay showed that alfa terpineol inhibited the growth of MCF-7 with
the IC50 value of 33μg/ml. Treatment using the dosage of the IC50 of alfa terpineol increased cell accumulation in Sub-G1 phase and caused early apoptotic
cell 5,92% compare to control. Evaluation of alfa terpineol decreased level of Bcl-2 and increased level of Bax. This result showed that alfa terpineol induce apoptosis through mechanism via mitochondrial pathway.
Conclusion: It is showed that alfa terpineol has the potential as anticancer agent and its mechanism are through inhibition of cell cycle and stimulation of apoptosis. |
| first_indexed | 2024-03-13T22:48:57Z |
| format | Thesis |
| id | oai:generic.eprints.org:119206 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:48:57Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1192062016-03-04T08:31:41Z https://repository.ugm.ac.id/119206/ AKTIVITAS ANTIKANKER ALFA TERPINEOL DARI GETAH Pinus merkusii (Jungh. Et de Vriese) PADA SIKLUS SEL, APOPTOSIS DAN EKSPRESI PROTEIN (Bcl-2 dan Bax) SEL LINI MCF-7 , Damiana Sapta Candrasari , Prof.dr. Sofia Mubarika Haryana ETD Background: Previous study showed that alfa terpineol from Pinus merkusii had cytotoxic effet on several cancer cell line namely T47D and HeLa. Objective: The aim of this study was to determine the effect of alfa terpineol on cytotoxicity, cell cycle changes, apoptotic and the changes expression of Bcl-2 and Bax in MCF-7 cell Methods: The cytotoxicity activity of alfa terpineol was determined using MTT assay, where the IC50 value was used as a parameter for cytotoxicity. Cell cycle changes was detected by Pi staining and flowcytometri. Annexin V-Pi staining and flowcytometry was used for detecting early apoptotic cell. The expression of Bcl-2 and Bax protein was explored by immunocytochemistry. Result: MTT assay showed that alfa terpineol inhibited the growth of MCF-7 with the IC50 value of 33μg/ml. Treatment using the dosage of the IC50 of alfa terpineol increased cell accumulation in Sub-G1 phase and caused early apoptotic cell 5,92% compare to control. Evaluation of alfa terpineol decreased level of Bcl-2 and increased level of Bax. This result showed that alfa terpineol induce apoptosis through mechanism via mitochondrial pathway. Conclusion: It is showed that alfa terpineol has the potential as anticancer agent and its mechanism are through inhibition of cell cycle and stimulation of apoptosis. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , Damiana Sapta Candrasari and , Prof.dr. Sofia Mubarika Haryana (2013) AKTIVITAS ANTIKANKER ALFA TERPINEOL DARI GETAH Pinus merkusii (Jungh. Et de Vriese) PADA SIKLUS SEL, APOPTOSIS DAN EKSPRESI PROTEIN (Bcl-2 dan Bax) SEL LINI MCF-7. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=59202 |
| spellingShingle | ETD , Damiana Sapta Candrasari , Prof.dr. Sofia Mubarika Haryana AKTIVITAS ANTIKANKER ALFA TERPINEOL DARI GETAH Pinus merkusii (Jungh. Et de Vriese) PADA SIKLUS SEL, APOPTOSIS DAN EKSPRESI PROTEIN (Bcl-2 dan Bax) SEL LINI MCF-7 |
| title | AKTIVITAS ANTIKANKER ALFA TERPINEOL DARI GETAH Pinus
merkusii (Jungh. Et de Vriese) PADA SIKLUS SEL, APOPTOSIS DAN
EKSPRESI PROTEIN (Bcl-2 dan Bax) SEL LINI MCF-7 |
| title_full | AKTIVITAS ANTIKANKER ALFA TERPINEOL DARI GETAH Pinus
merkusii (Jungh. Et de Vriese) PADA SIKLUS SEL, APOPTOSIS DAN
EKSPRESI PROTEIN (Bcl-2 dan Bax) SEL LINI MCF-7 |
| title_fullStr | AKTIVITAS ANTIKANKER ALFA TERPINEOL DARI GETAH Pinus
merkusii (Jungh. Et de Vriese) PADA SIKLUS SEL, APOPTOSIS DAN
EKSPRESI PROTEIN (Bcl-2 dan Bax) SEL LINI MCF-7 |
| title_full_unstemmed | AKTIVITAS ANTIKANKER ALFA TERPINEOL DARI GETAH Pinus
merkusii (Jungh. Et de Vriese) PADA SIKLUS SEL, APOPTOSIS DAN
EKSPRESI PROTEIN (Bcl-2 dan Bax) SEL LINI MCF-7 |
| title_short | AKTIVITAS ANTIKANKER ALFA TERPINEOL DARI GETAH Pinus
merkusii (Jungh. Et de Vriese) PADA SIKLUS SEL, APOPTOSIS DAN
EKSPRESI PROTEIN (Bcl-2 dan Bax) SEL LINI MCF-7 |
| title_sort | aktivitas antikanker alfa terpineol dari getah pinus merkusii jungh et de vriese pada siklus sel apoptosis dan ekspresi protein bcl 2 dan bax sel lini mcf 7 |
| topic | ETD |
| work_keys_str_mv | AT damianasaptacandrasari aktivitasantikankeralfaterpineoldarigetahpinusmerkusiijunghetdevriesepadasiklusselapoptosisdanekspresiproteinbcl2danbaxsellinimcf7 AT profdrsofiamubarikaharyana aktivitasantikankeralfaterpineoldarigetahpinusmerkusiijunghetdevriesepadasiklusselapoptosisdanekspresiproteinbcl2danbaxsellinimcf7 |