| Summary: | The extract of Katola bark is widely used in medicine, among others as an
antidiarrheal, antimicrobial and anti-inflammatory ingredients. Research has been
conducted to determine the anti-inflammatory activity of katola extract using
berberine HCl as an active ingredient standards. TLC and densitometer methods used
to standardize the content of the extract compounds using silica gel 60 F254 plates,
with eluent isopropanol: acetic acid: water (9: 0.5: 0.5v/v/v). Detection of spots using
UV light at 254 and 366 nm wave length and spray with reagent Dragendroff and
Cerium (IV) Sulfate.
Anti-inflammatory activity of katola extract determined with measure of
reducing of edema volume and ability to inhibit cyclooxigenase-2 (COX-2). The
measurement of edema volume was conducted with pletismometer of the left leg
back strain male rats on 0, 7, 14 and 21 days after inducted of CFA 0.1% in
subplantar. Immunohistochemical staining was conducted to determine the ability of
katola extract to inhibit of the enzyme cyclooxygenase-2 (COX-2). Animals devided
into 6 groups with each group consisting of 5 individuals. Group 1 is a negative
control, group 2 was given diclofenac sodium (9 mg/kg BW), the 3.4 and 5 were
given the extract in different doses divided the dose 114 mg/kg BW, the dose of 225
mg/kg BW and 450 mg/kg BW. The doses of 450 mg/kg BW of katola extract has
anti-inflammatory effects similar to diclofenac sodium 9 mg/kg BW. Katola extract
has rendemen 19.536% and relative concentration of alkaloids is 1=1 %
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