AKTIVITAS ANTIINFLAMASI EKSTRAK KAYU KATOLA (Arcangelisia flava (L.) Merr.) TERSTANDAR BERBERIN HCl PADA TIKUS GALUR WISTAR YANG DIINDUKSI COMPLETE FREUND�S ADJUVANT (CFA)
The extract of Katola bark is widely used in medicine, among others as an antidiarrheal, antimicrobial and anti-inflammatory ingredients. Research has been conducted to determine the anti-inflammatory activity of katola extract using berberine HCl as an active ingredient standards. TLC and densitome...
| Main Authors: | , |
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| _version_ | 1826046195360858112 |
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| author | , Hasnaeni , Prof. Dr. phil.nat. Sudarsono., Apt |
| author_facet | , Hasnaeni , Prof. Dr. phil.nat. Sudarsono., Apt |
| author_sort | , Hasnaeni |
| collection | UGM |
| description | The extract of Katola bark is widely used in medicine, among others as an
antidiarrheal, antimicrobial and anti-inflammatory ingredients. Research has been
conducted to determine the anti-inflammatory activity of katola extract using
berberine HCl as an active ingredient standards. TLC and densitometer methods used
to standardize the content of the extract compounds using silica gel 60 F254 plates,
with eluent isopropanol: acetic acid: water (9: 0.5: 0.5v/v/v). Detection of spots using
UV light at 254 and 366 nm wave length and spray with reagent Dragendroff and
Cerium (IV) Sulfate.
Anti-inflammatory activity of katola extract determined with measure of
reducing of edema volume and ability to inhibit cyclooxigenase-2 (COX-2). The
measurement of edema volume was conducted with pletismometer of the left leg
back strain male rats on 0, 7, 14 and 21 days after inducted of CFA 0.1% in
subplantar. Immunohistochemical staining was conducted to determine the ability of
katola extract to inhibit of the enzyme cyclooxygenase-2 (COX-2). Animals devided
into 6 groups with each group consisting of 5 individuals. Group 1 is a negative
control, group 2 was given diclofenac sodium (9 mg/kg BW), the 3.4 and 5 were
given the extract in different doses divided the dose 114 mg/kg BW, the dose of 225
mg/kg BW and 450 mg/kg BW. The doses of 450 mg/kg BW of katola extract has
anti-inflammatory effects similar to diclofenac sodium 9 mg/kg BW. Katola extract
has rendemen 19.536% and relative concentration of alkaloids is 1=1 % |
| first_indexed | 2024-03-13T22:49:21Z |
| format | Thesis |
| id | oai:generic.eprints.org:119338 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:49:21Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1193382016-03-04T08:38:42Z https://repository.ugm.ac.id/119338/ AKTIVITAS ANTIINFLAMASI EKSTRAK KAYU KATOLA (Arcangelisia flava (L.) Merr.) TERSTANDAR BERBERIN HCl PADA TIKUS GALUR WISTAR YANG DIINDUKSI COMPLETE FREUND�S ADJUVANT (CFA) , Hasnaeni , Prof. Dr. phil.nat. Sudarsono., Apt ETD The extract of Katola bark is widely used in medicine, among others as an antidiarrheal, antimicrobial and anti-inflammatory ingredients. Research has been conducted to determine the anti-inflammatory activity of katola extract using berberine HCl as an active ingredient standards. TLC and densitometer methods used to standardize the content of the extract compounds using silica gel 60 F254 plates, with eluent isopropanol: acetic acid: water (9: 0.5: 0.5v/v/v). Detection of spots using UV light at 254 and 366 nm wave length and spray with reagent Dragendroff and Cerium (IV) Sulfate. Anti-inflammatory activity of katola extract determined with measure of reducing of edema volume and ability to inhibit cyclooxigenase-2 (COX-2). The measurement of edema volume was conducted with pletismometer of the left leg back strain male rats on 0, 7, 14 and 21 days after inducted of CFA 0.1% in subplantar. Immunohistochemical staining was conducted to determine the ability of katola extract to inhibit of the enzyme cyclooxygenase-2 (COX-2). Animals devided into 6 groups with each group consisting of 5 individuals. Group 1 is a negative control, group 2 was given diclofenac sodium (9 mg/kg BW), the 3.4 and 5 were given the extract in different doses divided the dose 114 mg/kg BW, the dose of 225 mg/kg BW and 450 mg/kg BW. The doses of 450 mg/kg BW of katola extract has anti-inflammatory effects similar to diclofenac sodium 9 mg/kg BW. Katola extract has rendemen 19.536% and relative concentration of alkaloids is 1=1 % [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , Hasnaeni and , Prof. Dr. phil.nat. Sudarsono., Apt (2013) AKTIVITAS ANTIINFLAMASI EKSTRAK KAYU KATOLA (Arcangelisia flava (L.) Merr.) TERSTANDAR BERBERIN HCl PADA TIKUS GALUR WISTAR YANG DIINDUKSI COMPLETE FREUND�S ADJUVANT (CFA). UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=59334 |
| spellingShingle | ETD , Hasnaeni , Prof. Dr. phil.nat. Sudarsono., Apt AKTIVITAS ANTIINFLAMASI EKSTRAK KAYU KATOLA (Arcangelisia flava (L.) Merr.) TERSTANDAR BERBERIN HCl PADA TIKUS GALUR WISTAR YANG DIINDUKSI COMPLETE FREUND�S ADJUVANT (CFA) |
| title | AKTIVITAS ANTIINFLAMASI EKSTRAK KAYU KATOLA (Arcangelisia
flava (L.) Merr.) TERSTANDAR BERBERIN HCl PADA TIKUS GALUR
WISTAR YANG DIINDUKSI COMPLETE FREUND�S ADJUVANT (CFA) |
| title_full | AKTIVITAS ANTIINFLAMASI EKSTRAK KAYU KATOLA (Arcangelisia
flava (L.) Merr.) TERSTANDAR BERBERIN HCl PADA TIKUS GALUR
WISTAR YANG DIINDUKSI COMPLETE FREUND�S ADJUVANT (CFA) |
| title_fullStr | AKTIVITAS ANTIINFLAMASI EKSTRAK KAYU KATOLA (Arcangelisia
flava (L.) Merr.) TERSTANDAR BERBERIN HCl PADA TIKUS GALUR
WISTAR YANG DIINDUKSI COMPLETE FREUND�S ADJUVANT (CFA) |
| title_full_unstemmed | AKTIVITAS ANTIINFLAMASI EKSTRAK KAYU KATOLA (Arcangelisia
flava (L.) Merr.) TERSTANDAR BERBERIN HCl PADA TIKUS GALUR
WISTAR YANG DIINDUKSI COMPLETE FREUND�S ADJUVANT (CFA) |
| title_short | AKTIVITAS ANTIINFLAMASI EKSTRAK KAYU KATOLA (Arcangelisia
flava (L.) Merr.) TERSTANDAR BERBERIN HCl PADA TIKUS GALUR
WISTAR YANG DIINDUKSI COMPLETE FREUND�S ADJUVANT (CFA) |
| title_sort | aktivitas antiinflamasi ekstrak kayu katola arcangelisia flava l merr terstandar berberin hcl pada tikus galur wistar yang diinduksi complete freunda��s adjuvant cfa |
| topic | ETD |
| work_keys_str_mv | AT hasnaeni aktivitasantiinflamasiekstrakkayukatolaarcangelisiaflavalmerrterstandarberberinhclpadatikusgalurwistaryangdiinduksicompletefreundasadjuvantcfa AT profdrphilnatsudarsonoapt aktivitasantiinflamasiekstrakkayukatolaarcangelisiaflavalmerrterstandarberberinhclpadatikusgalurwistaryangdiinduksicompletefreundasadjuvantcfa |