PERBANDINGAN EKSPRESI PROTEIN Bak DAN INDEKS APOPTOSIS SEL TROFOBLAS PADA KEHAMILAN PREEKLAMPSIA BERAT DAN KEHAMILAN NORMOTENSI
Background: Placental is a central and important focus on the pathogenesis of preeclampsia/eclampsia. Failure of trophoblast cell to the spiral arteries remodeling process due to excessive apoptosis causes uteroplacenter ischemia and damage of endothelial cells that give rise to clinical manifestasi...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| _version_ | 1797033211678687232 |
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| author | , Marihot Pasaribu , dr. Diah Rumekti Hadiati, M.Sc SpOG(K) |
| author_facet | , Marihot Pasaribu , dr. Diah Rumekti Hadiati, M.Sc SpOG(K) |
| author_sort | , Marihot Pasaribu |
| collection | UGM |
| description | Background: Placental is a central and important focus on the pathogenesis of
preeclampsia/eclampsia. Failure of trophoblast cell to the spiral arteries
remodeling process due to excessive apoptosis causes uteroplacenter ischemia and
damage of endothelial cells that give rise to clinical manifestasions of preeclampsia/
eclampsia. Excessive trophoblast apoptosis in preeclampsia/eclampsia
mainly occurs through the intrinsic pathway in which expression of Bak protein
increases mitochondrial membranes permeability of cytochrome C which further
activated the caspase cascade and become involved in the process of cell death.
Objective of study: To compare the expression of Bak protein and trophoblastic
apoptotic index between severe preeclampsia and normotensive pregnancies.
Methods: Cross sectional study which consist of 43 severe preeclampsia
pregnancies and 38 normotensive pregnancies recruited between October 2011-
March 2012. Trophoblastic cells Bak protein expression is measured by
imunohistochemical staining technique and trophoblast apoptotic index
quantification is examined by TUNEL assay.
Results: There are significant differences of Bak protein expression in placental
tissue of preeclampsia group (2.37±0.91, p=0.012) compared by normotensive
group (1.92�0.59). There are significant differences of apoptotic index in
placental tissue of preeclampsia group (0.44 0 ± .26, p=0.00) compared by
normotensive group (0.24�0.20).
Conclusion: The Bak protein expression and the number of apoptotic cells in
trophoblast tissue of preeclampsia complicated pregnancy were higher than
normal pregnancy. |
| first_indexed | 2024-03-13T22:50:36Z |
| format | Thesis |
| id | oai:generic.eprints.org:119730 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:50:36Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1197302016-03-04T08:40:44Z https://repository.ugm.ac.id/119730/ PERBANDINGAN EKSPRESI PROTEIN Bak DAN INDEKS APOPTOSIS SEL TROFOBLAS PADA KEHAMILAN PREEKLAMPSIA BERAT DAN KEHAMILAN NORMOTENSI , Marihot Pasaribu , dr. Diah Rumekti Hadiati, M.Sc SpOG(K) ETD Background: Placental is a central and important focus on the pathogenesis of preeclampsia/eclampsia. Failure of trophoblast cell to the spiral arteries remodeling process due to excessive apoptosis causes uteroplacenter ischemia and damage of endothelial cells that give rise to clinical manifestasions of preeclampsia/ eclampsia. Excessive trophoblast apoptosis in preeclampsia/eclampsia mainly occurs through the intrinsic pathway in which expression of Bak protein increases mitochondrial membranes permeability of cytochrome C which further activated the caspase cascade and become involved in the process of cell death. Objective of study: To compare the expression of Bak protein and trophoblastic apoptotic index between severe preeclampsia and normotensive pregnancies. Methods: Cross sectional study which consist of 43 severe preeclampsia pregnancies and 38 normotensive pregnancies recruited between October 2011- March 2012. Trophoblastic cells Bak protein expression is measured by imunohistochemical staining technique and trophoblast apoptotic index quantification is examined by TUNEL assay. Results: There are significant differences of Bak protein expression in placental tissue of preeclampsia group (2.37±0.91, p=0.012) compared by normotensive group (1.92�0.59). There are significant differences of apoptotic index in placental tissue of preeclampsia group (0.44 0 ± .26, p=0.00) compared by normotensive group (0.24�0.20). Conclusion: The Bak protein expression and the number of apoptotic cells in trophoblast tissue of preeclampsia complicated pregnancy were higher than normal pregnancy. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , Marihot Pasaribu and , dr. Diah Rumekti Hadiati, M.Sc SpOG(K) (2013) PERBANDINGAN EKSPRESI PROTEIN Bak DAN INDEKS APOPTOSIS SEL TROFOBLAS PADA KEHAMILAN PREEKLAMPSIA BERAT DAN KEHAMILAN NORMOTENSI. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=59733 |
| spellingShingle | ETD , Marihot Pasaribu , dr. Diah Rumekti Hadiati, M.Sc SpOG(K) PERBANDINGAN EKSPRESI PROTEIN Bak DAN INDEKS APOPTOSIS SEL TROFOBLAS PADA KEHAMILAN PREEKLAMPSIA BERAT DAN KEHAMILAN NORMOTENSI |
| title | PERBANDINGAN EKSPRESI PROTEIN Bak DAN INDEKS APOPTOSIS SEL TROFOBLAS PADA KEHAMILAN PREEKLAMPSIA BERAT DAN KEHAMILAN NORMOTENSI |
| title_full | PERBANDINGAN EKSPRESI PROTEIN Bak DAN INDEKS APOPTOSIS SEL TROFOBLAS PADA KEHAMILAN PREEKLAMPSIA BERAT DAN KEHAMILAN NORMOTENSI |
| title_fullStr | PERBANDINGAN EKSPRESI PROTEIN Bak DAN INDEKS APOPTOSIS SEL TROFOBLAS PADA KEHAMILAN PREEKLAMPSIA BERAT DAN KEHAMILAN NORMOTENSI |
| title_full_unstemmed | PERBANDINGAN EKSPRESI PROTEIN Bak DAN INDEKS APOPTOSIS SEL TROFOBLAS PADA KEHAMILAN PREEKLAMPSIA BERAT DAN KEHAMILAN NORMOTENSI |
| title_short | PERBANDINGAN EKSPRESI PROTEIN Bak DAN INDEKS APOPTOSIS SEL TROFOBLAS PADA KEHAMILAN PREEKLAMPSIA BERAT DAN KEHAMILAN NORMOTENSI |
| title_sort | perbandingan ekspresi protein bak dan indeks apoptosis sel trofoblas pada kehamilan preeklampsia berat dan kehamilan normotensi |
| topic | ETD |
| work_keys_str_mv | AT marihotpasaribu perbandinganekspresiproteinbakdanindeksapoptosisseltrofoblaspadakehamilanpreeklampsiaberatdankehamilannormotensi AT drdiahrumektihadiatimscspogk perbandinganekspresiproteinbakdanindeksapoptosisseltrofoblaspadakehamilanpreeklampsiaberatdankehamilannormotensi |