PENGARUH DERAJAT DEASETILASI TERHADAP EFEKTIVITAS CHITOSAN BEAD SEBAGAI PADATAN PENDUKUNG IMOBILISASI LIPASE DENGAN TEKNIK PENGAITAN SILA
The effect of deacetylation degree of chitosan on the effectiveness of chitosan beads as solid support immobilized lipase by cross-linking techique has been carried out. The study aims to obtain optimal conditions for immobilization of lipase on chitosan beads. Chitosan beads made from the chitosan...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| _version_ | 1797033269736243200 |
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| author | , LIZMA FEBRINA , Dr. Tri Joko Raharjo, M.Si. |
| author_facet | , LIZMA FEBRINA , Dr. Tri Joko Raharjo, M.Si. |
| author_sort | , LIZMA FEBRINA |
| collection | UGM |
| description | The effect of deacetylation degree of chitosan on the effectiveness of chitosan beads as solid support immobilized lipase by cross-linking techique has been carried out. The study aims to obtain optimal conditions for immobilization of lipase on chitosan beads. Chitosan beads made from the chitosan powder with various degree of deacetylation by inverse phase method. The study starts from the optimization process of immobilization such as phosphate buffer pH and concentration of glutaraldehyde as a cross-linker. Lipase immobilized on chitosan beads was tested activities, thermal stability, re-uses and parameter kinetics on the hydrolysis reaction of palm oil triglycerides.
Phospate buffer with pH 6 is optimal condition to dissolved lipase enzym. DD chitosan that resulted are 80,71%, 87,51% and 91,77%. Optimum concentration of glutaraldehyde optimal is approached in glutaraldehyde 0,5%(v/v) for DD chitosan bead 80,71% with (%) percentage immobilization enzyme 84% |
| first_indexed | 2024-03-13T22:51:22Z |
| format | Thesis |
| id | oai:generic.eprints.org:119978 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:51:22Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1199782016-03-04T08:39:51Z https://repository.ugm.ac.id/119978/ PENGARUH DERAJAT DEASETILASI TERHADAP EFEKTIVITAS CHITOSAN BEAD SEBAGAI PADATAN PENDUKUNG IMOBILISASI LIPASE DENGAN TEKNIK PENGAITAN SILA , LIZMA FEBRINA , Dr. Tri Joko Raharjo, M.Si. ETD The effect of deacetylation degree of chitosan on the effectiveness of chitosan beads as solid support immobilized lipase by cross-linking techique has been carried out. The study aims to obtain optimal conditions for immobilization of lipase on chitosan beads. Chitosan beads made from the chitosan powder with various degree of deacetylation by inverse phase method. The study starts from the optimization process of immobilization such as phosphate buffer pH and concentration of glutaraldehyde as a cross-linker. Lipase immobilized on chitosan beads was tested activities, thermal stability, re-uses and parameter kinetics on the hydrolysis reaction of palm oil triglycerides. Phospate buffer with pH 6 is optimal condition to dissolved lipase enzym. DD chitosan that resulted are 80,71%, 87,51% and 91,77%. Optimum concentration of glutaraldehyde optimal is approached in glutaraldehyde 0,5%(v/v) for DD chitosan bead 80,71% with (%) percentage immobilization enzyme 84% [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , LIZMA FEBRINA and , Dr. Tri Joko Raharjo, M.Si. (2013) PENGARUH DERAJAT DEASETILASI TERHADAP EFEKTIVITAS CHITOSAN BEAD SEBAGAI PADATAN PENDUKUNG IMOBILISASI LIPASE DENGAN TEKNIK PENGAITAN SILA. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=59985 |
| spellingShingle | ETD , LIZMA FEBRINA , Dr. Tri Joko Raharjo, M.Si. PENGARUH DERAJAT DEASETILASI TERHADAP EFEKTIVITAS CHITOSAN BEAD SEBAGAI PADATAN PENDUKUNG IMOBILISASI LIPASE DENGAN TEKNIK PENGAITAN SILA |
| title | PENGARUH DERAJAT DEASETILASI TERHADAP EFEKTIVITAS CHITOSAN BEAD SEBAGAI PADATAN PENDUKUNG IMOBILISASI LIPASE DENGAN TEKNIK PENGAITAN SILA |
| title_full | PENGARUH DERAJAT DEASETILASI TERHADAP EFEKTIVITAS CHITOSAN BEAD SEBAGAI PADATAN PENDUKUNG IMOBILISASI LIPASE DENGAN TEKNIK PENGAITAN SILA |
| title_fullStr | PENGARUH DERAJAT DEASETILASI TERHADAP EFEKTIVITAS CHITOSAN BEAD SEBAGAI PADATAN PENDUKUNG IMOBILISASI LIPASE DENGAN TEKNIK PENGAITAN SILA |
| title_full_unstemmed | PENGARUH DERAJAT DEASETILASI TERHADAP EFEKTIVITAS CHITOSAN BEAD SEBAGAI PADATAN PENDUKUNG IMOBILISASI LIPASE DENGAN TEKNIK PENGAITAN SILA |
| title_short | PENGARUH DERAJAT DEASETILASI TERHADAP EFEKTIVITAS CHITOSAN BEAD SEBAGAI PADATAN PENDUKUNG IMOBILISASI LIPASE DENGAN TEKNIK PENGAITAN SILA |
| title_sort | pengaruh derajat deasetilasi terhadap efektivitas chitosan bead sebagai padatan pendukung imobilisasi lipase dengan teknik pengaitan sila |
| topic | ETD |
| work_keys_str_mv | AT lizmafebrina pengaruhderajatdeasetilasiterhadapefektivitaschitosanbeadsebagaipadatanpendukungimobilisasilipasedenganteknikpengaitansila AT drtrijokoraharjomsi pengaruhderajatdeasetilasiterhadapefektivitaschitosanbeadsebagaipadatanpendukungimobilisasilipasedenganteknikpengaitansila |