ISOLASI BAKTERI PROTEOLITIK AIR BUDIDAYA IKAN DAN KEMAMPUANNYA DALAM HIDROLISIS PROTEIN

Proteolytic bacteria is a group ofbacteria that play role in degrading protein and could be used in decreasing protein in aquaculture pond. This research was aimed to obtain isolates of proteolytic bacteria thathave high capability in hydrolyzing protein from aquaculture ponds. Sources of the bac...

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Main Authors: , ARIFA ZUCHROTUNNISA, , Dr. Ir. Ngadiman, M.Si.
Format: Thesis
Published: [Yogyakarta] : Universitas Gadjah Mada 2013
Subjects:
ETD
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author , ARIFA ZUCHROTUNNISA
, Dr. Ir. Ngadiman, M.Si.
author_facet , ARIFA ZUCHROTUNNISA
, Dr. Ir. Ngadiman, M.Si.
author_sort , ARIFA ZUCHROTUNNISA
collection UGM
description Proteolytic bacteria is a group ofbacteria that play role in degrading protein and could be used in decreasing protein in aquaculture pond. This research was aimed to obtain isolates of proteolytic bacteria thathave high capability in hydrolyzing protein from aquaculture ponds. Sources of the bacteria were sludge and water mixture of milkfish, shrimp, african catfish, and giant gouramy aquaculture ponds. The proteolytic bacteria were isolated by pour plating on skim milk agar medium. Qualitative selection was based on hydrolysis zone in the skim milk agar medium, and quantitative selection was done by Lowry method. Morphological and physiological tests were also observed to determine the isolates characters. In vitrotest on natural media was assayed to know proteolytic activity of the selected isolates. Eighty four proteolytic bacteria were isolated from the samples among them, sixteenisolates were selected having specific enzyme activity 1,76 - 20,196 unit/mg protein. The selected isolates have various morphological and physiological characters. Introduction of the selected isolates in aquaculture water of milkfish, shrimp, giant gouramy, and african catfish could increase the protein degradation rate 35, 36, 25, and 55 %.
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institution Universiti Gadjah Mada
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spelling oai:generic.eprints.org:1200192016-03-04T08:24:07Z https://repository.ugm.ac.id/120019/ ISOLASI BAKTERI PROTEOLITIK AIR BUDIDAYA IKAN DAN KEMAMPUANNYA DALAM HIDROLISIS PROTEIN , ARIFA ZUCHROTUNNISA , Dr. Ir. Ngadiman, M.Si. ETD Proteolytic bacteria is a group ofbacteria that play role in degrading protein and could be used in decreasing protein in aquaculture pond. This research was aimed to obtain isolates of proteolytic bacteria thathave high capability in hydrolyzing protein from aquaculture ponds. Sources of the bacteria were sludge and water mixture of milkfish, shrimp, african catfish, and giant gouramy aquaculture ponds. The proteolytic bacteria were isolated by pour plating on skim milk agar medium. Qualitative selection was based on hydrolysis zone in the skim milk agar medium, and quantitative selection was done by Lowry method. Morphological and physiological tests were also observed to determine the isolates characters. In vitrotest on natural media was assayed to know proteolytic activity of the selected isolates. Eighty four proteolytic bacteria were isolated from the samples among them, sixteenisolates were selected having specific enzyme activity 1,76 - 20,196 unit/mg protein. The selected isolates have various morphological and physiological characters. Introduction of the selected isolates in aquaculture water of milkfish, shrimp, giant gouramy, and african catfish could increase the protein degradation rate 35, 36, 25, and 55 %. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , ARIFA ZUCHROTUNNISA and , Dr. Ir. Ngadiman, M.Si. (2013) ISOLASI BAKTERI PROTEOLITIK AIR BUDIDAYA IKAN DAN KEMAMPUANNYA DALAM HIDROLISIS PROTEIN. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=60026
spellingShingle ETD
, ARIFA ZUCHROTUNNISA
, Dr. Ir. Ngadiman, M.Si.
ISOLASI BAKTERI PROTEOLITIK AIR BUDIDAYA IKAN DAN KEMAMPUANNYA DALAM HIDROLISIS PROTEIN
title ISOLASI BAKTERI PROTEOLITIK AIR BUDIDAYA IKAN DAN KEMAMPUANNYA DALAM HIDROLISIS PROTEIN
title_full ISOLASI BAKTERI PROTEOLITIK AIR BUDIDAYA IKAN DAN KEMAMPUANNYA DALAM HIDROLISIS PROTEIN
title_fullStr ISOLASI BAKTERI PROTEOLITIK AIR BUDIDAYA IKAN DAN KEMAMPUANNYA DALAM HIDROLISIS PROTEIN
title_full_unstemmed ISOLASI BAKTERI PROTEOLITIK AIR BUDIDAYA IKAN DAN KEMAMPUANNYA DALAM HIDROLISIS PROTEIN
title_short ISOLASI BAKTERI PROTEOLITIK AIR BUDIDAYA IKAN DAN KEMAMPUANNYA DALAM HIDROLISIS PROTEIN
title_sort isolasi bakteri proteolitik air budidaya ikan dan kemampuannya dalam hidrolisis protein
topic ETD
work_keys_str_mv AT arifazuchrotunnisa isolasibakteriproteolitikairbudidayaikandankemampuannyadalamhidrolisisprotein
AT drirngadimanmsi isolasibakteriproteolitikairbudidayaikandankemampuannyadalamhidrolisisprotein