| Summary: | Diabetes mellitus define as metabolic disorder with chronic hyperglycemia
and disturbance of carbohydrate, lipid and protein metabolism. This also caused
by insulin deficiency, insulin resistance or islets beta cell failure to produce
insulin. DM insulin deficiency linked with beta cell function in insulin synthetase.
Patient with DM therapy is important to know which metabolic mechanism that
disorder. This present study is to investigate effectivity of α-amylase and α-
glukosidase inhibition, blood glucose concentration decrease and pancreatic effect
from andrographolide for therapy on diabetes mellitus insulin deficiency rats.
Experimental methode that used in this study was in vitro enzymatic activity
of andrographolide series concentration versus α-amylase and α-glukosidase
inhibition of amylum and p-nitrophenil-α-D-glucopiranosyde as substrate. Also, in
vivo study of insulin deficiency rats inducted by streptozotocyn. Confirmed
parameter was inhibitory prosentase of the ezymes and blood glucose
concentration measurement with spectrophotometer, include pancreas histologies
stained with HE, VB and IHC protein insulin expression.
Result of this study, andrographolide inhibitory activity potential for α-
amylase and α-glukosidase was not significant. Andrographolide with 1,5 and 4,5
mg/kgbw concentration consecutively decrease blood glucose estimate 39,18%
and 32,51%. It is also influence to pancreas histologies insulin deficiency rats,
consecutively 33% degeneration decrease of islet cell, beta cell number of islet
estimate 29% and 58% higher than negative control, also protein insulin
expression estimate 81% and 78% concentrated than negative control with 58%.
|
|---|