| Summary: | In human, E. coli of VTEC group may cause diarhoea, hemorrhagic colitis,
hemolytic uremic syndrome (HUS) and death. VTEC was transmitted to human by consuming the milk contaminated by E.coli from the cow and environment. Previous research reported 43% of feces samples were detected VTEC. The aim of the research was to identify the risk factor of contaminated milk by E. coli, and to estimate the risk factor on the farm level.
Based on six farmer samples were studied VTEC status on the previous
research. Specimens were the milk, water, feeds, hand swab, filter swab, milkcans swab, and feces. The questioners were taken to collect the risk factor information.
Specimens were taken three times in the morning milking. The total sample were 240 ( 72 milks, 72 swab, 36 water, 18 feeds, and 42 feces). The research methods were isolation and identification of E. coli, and E. coli count (Most Probable Number 3 x 3 table). Isolation and identification of E.coli had be done with coliform estimation test, coliform assertion test, E. coli estimation test, E. coli confirmation test and biochemical testing with IMVIC. The questioners were included farmer data, cows data, risk factor before and after milking, feed-water risk factor, and farmer knowledge of milking hygiene. The data obtained from the questioner and the isolation of E.coli was analyzed descriptively. Determination of the risk factor of the contaminated milk was analyzed by the linear regression using the SPSS programme.
The result showed that MPN of E. coli in milk from the dairy farmers to the
bulk tank has increased. Contamination levels of E. coli in the milk were log 0,70 MPN/ml) from the cow teats, log 0,83 MPN/ml from the milkcans, log 1,02 MPN/ml from the bulk tank of the milk shelter, and log 1,06 MPN/ml in the cooling unit. The factor increasing the MPN level of the milk from the teats was the hand swab before oiling [logSS = 0,004 + 0,486 (logSWTA)], that of the milkcan was filter swab [logSMC = 0,010 + 0,187 (logSWSAR)], and that of cooling unit was bulk tank shelters [logSCU = 0,183 + 0,866 (logSTPS)].
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