RESPONS IMUNITAS IKAN MAS (Cyprinus carpio Linn.) PADA PEMBERIAN ASAM HUMAT TANAH GAMBUT KALIMANTAN
Indonesia as the 4th largest country in peat soil land has many potencies of humic acid substance to explore. Humic acid has been known has many biological activities such as immunostimulatory effect. The aim of this study was to know the effect of humic acid from Borneo peat soil to immune respons...
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Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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author | , Diah Wulandari Rousdy , Dr. biol. hom. Nastiti Wijayanti |
author_facet | , Diah Wulandari Rousdy , Dr. biol. hom. Nastiti Wijayanti |
author_sort | , Diah Wulandari Rousdy |
collection | UGM |
description | Indonesia as the 4th largest country in peat soil land has many potencies of humic acid substance to explore. Humic acid has been known has many biological activities such as immunostimulatory effect. The aim of this study was to know the effect of humic acid from Borneo peat soil to immune respons of carp (Cyprinus carpio Linn.). Research design using RCD (Randomized Complete Design) with six treatment and three replication. The treatment are normal control group, negative control group, positive control group and three group treatment with humic acid (1%, 3% and 5%). Humic acid was given orally to fish for 21 days and Aeromonas hydrophila was used in the challenge test. The results of the study showed administration of humic acid orally enhanced nonspecific and specific immune responses depend on the dose. One percent humic acid treatments stimulate the nonspecific and specific immune system as seen from the number of leukocytes, phagocytic activity, production of ROS after infection, antibody titers and lymphocyte percentage. While humic acid 3% and 5% also enhanced nonspecific immune system by increasing the number of leukocytes, phagocytic activity, the number of active phagocytic cells. Specific immune responses in the treatment of humic acid 3% and 5% is not as good as 1% humic acid treatment. On the other hand, administration of humic acid, except humic acid 1%, caused a decrease in blood hemoglobin levels, ROS production and granulocytes monocytes percentage. There were no significant differences among groups treatment. Survival rate (SR) of carp after infected by A. hydrophila in humic acid concentration of 1% was higher than other treatment. This result indicated humic acid was potential to enhance immune system of fish. |
first_indexed | 2024-03-13T22:54:23Z |
format | Thesis |
id | oai:generic.eprints.org:120941 |
institution | Universiti Gadjah Mada |
last_indexed | 2024-03-13T22:54:23Z |
publishDate | 2013 |
publisher | [Yogyakarta] : Universitas Gadjah Mada |
record_format | dspace |
spelling | oai:generic.eprints.org:1209412016-03-04T08:32:20Z https://repository.ugm.ac.id/120941/ RESPONS IMUNITAS IKAN MAS (Cyprinus carpio Linn.) PADA PEMBERIAN ASAM HUMAT TANAH GAMBUT KALIMANTAN , Diah Wulandari Rousdy , Dr. biol. hom. Nastiti Wijayanti ETD Indonesia as the 4th largest country in peat soil land has many potencies of humic acid substance to explore. Humic acid has been known has many biological activities such as immunostimulatory effect. The aim of this study was to know the effect of humic acid from Borneo peat soil to immune respons of carp (Cyprinus carpio Linn.). Research design using RCD (Randomized Complete Design) with six treatment and three replication. The treatment are normal control group, negative control group, positive control group and three group treatment with humic acid (1%, 3% and 5%). Humic acid was given orally to fish for 21 days and Aeromonas hydrophila was used in the challenge test. The results of the study showed administration of humic acid orally enhanced nonspecific and specific immune responses depend on the dose. One percent humic acid treatments stimulate the nonspecific and specific immune system as seen from the number of leukocytes, phagocytic activity, production of ROS after infection, antibody titers and lymphocyte percentage. While humic acid 3% and 5% also enhanced nonspecific immune system by increasing the number of leukocytes, phagocytic activity, the number of active phagocytic cells. Specific immune responses in the treatment of humic acid 3% and 5% is not as good as 1% humic acid treatment. On the other hand, administration of humic acid, except humic acid 1%, caused a decrease in blood hemoglobin levels, ROS production and granulocytes monocytes percentage. There were no significant differences among groups treatment. Survival rate (SR) of carp after infected by A. hydrophila in humic acid concentration of 1% was higher than other treatment. This result indicated humic acid was potential to enhance immune system of fish. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , Diah Wulandari Rousdy and , Dr. biol. hom. Nastiti Wijayanti (2013) RESPONS IMUNITAS IKAN MAS (Cyprinus carpio Linn.) PADA PEMBERIAN ASAM HUMAT TANAH GAMBUT KALIMANTAN. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=60979 |
spellingShingle | ETD , Diah Wulandari Rousdy , Dr. biol. hom. Nastiti Wijayanti RESPONS IMUNITAS IKAN MAS (Cyprinus carpio Linn.) PADA PEMBERIAN ASAM HUMAT TANAH GAMBUT KALIMANTAN |
title | RESPONS IMUNITAS IKAN MAS (Cyprinus carpio Linn.) PADA PEMBERIAN ASAM HUMAT TANAH GAMBUT KALIMANTAN |
title_full | RESPONS IMUNITAS IKAN MAS (Cyprinus carpio Linn.) PADA PEMBERIAN ASAM HUMAT TANAH GAMBUT KALIMANTAN |
title_fullStr | RESPONS IMUNITAS IKAN MAS (Cyprinus carpio Linn.) PADA PEMBERIAN ASAM HUMAT TANAH GAMBUT KALIMANTAN |
title_full_unstemmed | RESPONS IMUNITAS IKAN MAS (Cyprinus carpio Linn.) PADA PEMBERIAN ASAM HUMAT TANAH GAMBUT KALIMANTAN |
title_short | RESPONS IMUNITAS IKAN MAS (Cyprinus carpio Linn.) PADA PEMBERIAN ASAM HUMAT TANAH GAMBUT KALIMANTAN |
title_sort | respons imunitas ikan mas cyprinus carpio linn pada pemberian asam humat tanah gambut kalimantan |
topic | ETD |
work_keys_str_mv | AT diahwulandarirousdy responsimunitasikanmascyprinuscarpiolinnpadapemberianasamhumattanahgambutkalimantan AT drbiolhomnastitiwijayanti responsimunitasikanmascyprinuscarpiolinnpadapemberianasamhumattanahgambutkalimantan |