| Summary: | Trichomonosis is a parasitic diasease caused by Trichomonas gallinae that
inhabit anterior digestive tract of columbids. Genetic analysis is needed to
determine whether isolates differed genetically depending on geographical
location using ribosomal DNA (rDNA) sequences. The aim of this research is to
identify T. gallinae based on rDNA sequences by DNA sequencing to analyze the
phylogenetic relationships.
The DNA sample were isolated from esophageal mucus of pigeon in
Yogyakarta. Amplification of rDNA by PCR technique used TRI_1F and TRI_1R
primers under condition of pre-denaturation 94°C for 3 min, denaturation 94ºC for
30 s, annealing 52ºC for 45 s, extension 72 ºC for 1 min, and final extension 72 ºC
for 5 min at 35 cycles. The PCR reaction produced 351 bp DNA segment which
has followed by sequencing. Then result of rDNA segment sequences would be
treated in multiple alignment with other protozoas taken from Genbank and then
analyzed using MEGA5.1
Result of this research showed that the sample has closer relationship with
isolate Colorado and Spain A from Genbank indicated by genetic distance of 0-
0,3%.
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