| Summary: | Mastitis is one of diseases in dairy cows that can cause a loss for a breeder,
especially decreasing number of production and milk quality. Inappropriate
antibiotics application may cause bacterial resistance. The research is aimed to
detect sensitivity of Staphylococcus aureus towards penicillin G and tetracycline.
Sixteen samples of cow�s milk were cultured on Blood Agar Plate (BP)
and Mac Conkey Agar (MCA) was then incubated for 24 hours at 37ºC. Coloni
that grow were identified on Gram staining, catalase test, coagulase test and sugar
fermentation test. To determine the sensitivity to the antibiotic penicillin G and
Tetracycline bacteria that have been cultured on media BHI (Brain Heart
Infussion) then cultured average detail in MHA (Muller Hinton Agar) and
incubated at 37ºC for 24 hours with upend position. Resistant zone formed was
measured with rule. The result was matched with interpreting tables standardized
resistant zone Kirby-Baue to determine S. aureus sensitivity towards the antibiotic
penicillin G and Tetracycline.
The result of isolation shows that from 15 samples tested, 2 samples are
positively infected by S. aureus. Test result of Staphylococcus sensitivity towards
Penicillin G and Tetracycline shows that 2 samples of S. aureus are positively
sensitive towards Penicillin G and Tetracycline. According to sensitivity test in
this research, the researcher suggests to use Penicillin G and Tetracycline in
subclinical mastitis treatment caused by Staphylococcus aureus.
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