ISOLASI DAN IDENTIFIKASI VIRUS EGG DROP SYNDROME PADA AYAM LAYER
Egg Drop Syndrome (EDS) is a disease that attacks the layer hen in production phase and causes the failing of peak production, the decreasing production, and the presence of an egg without a shell. This study was aimed to isolate and identify the EDS virus in chicken layer that was diagnosed...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| _version_ | 1826046558356897792 |
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| author | , FIDYAH FITRAWATI , Dr. drh. Michael Haryadi Wibowo, M.P. |
| author_facet | , FIDYAH FITRAWATI , Dr. drh. Michael Haryadi Wibowo, M.P. |
| author_sort | , FIDYAH FITRAWATI |
| collection | UGM |
| description | Egg Drop Syndrome (EDS) is a disease that attacks the layer hen in
production phase and causes the failing of peak production, the decreasing
production, and the presence of an egg without a shell. This study was aimed to
isolate and identify the EDS virus in chicken layer that was diagnosed as a disease
of EDS by hemagglutination (HA) and hemagglutination inhibition (HI) assay.
The sample used was chicken�s uterus and residual water of egg washing
from FF/Sl eman/2011, along with uterus from specific pathogen free (SPF) layer
chicken that was infected by SR/WNO/2011. Specific pathogen free layer chicken
which was from Bandung and was passing through the production phase was fed
with food which was mixed with egg without shell from SR/WNO/2011. The
chicken together with with chicken FF/Sleman/2011 were dissected when
symptoms of infection shown and the uterus taken to make it as the sample.
Symptoms of infection was shown by the presence of dents or pale eggs.
Infundibulum of chicken FF/Sleman/2011 was explored and was found out the
egg without eggshell. Then the egg was washed using sterile PBS and became the
sample. The three subsequent samples was propagated in allantois fluid of
embryonated duck eggs that was 16 days. Allantoic fluid was harvested after
incubation for 4 days, then it was tested by with HA and HI assay. HA and HI test
with EDS anti serum used chicken�s eritrosit in percentage 0,8. HA test positive
result was indicated by erythrocyte cell aggregates formed by the vir al
hemagglutinin protein binding to erythrocyte surface receptors. This assay was
different with HI assay that uses anti serum Avian Influenza (AI), Newcastle
disease (ND), and EDS to test suitability of the virus with that anti serum. The
specific anti serum would bind with that virus and block the hemagglutination
reaction.
HA test in uterus sample of both SR/WNO/2011 and FF/Sleman/2011
showed titer 23 HA units and egg wash water sample of FF/Sleman/2011 showed
titer 22 HA units. HI test for comparison with anti serum ND and AI were
negative, while tests with anti serum EDS showed positive results. Based on HA
and HI test results, we can conclude that the virus which grew in allantoic fluid
was EDS virus. |
| first_indexed | 2024-03-13T22:54:54Z |
| format | Thesis |
| id | oai:generic.eprints.org:121112 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:54:54Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1211122016-03-04T08:35:50Z https://repository.ugm.ac.id/121112/ ISOLASI DAN IDENTIFIKASI VIRUS EGG DROP SYNDROME PADA AYAM LAYER , FIDYAH FITRAWATI , Dr. drh. Michael Haryadi Wibowo, M.P. ETD Egg Drop Syndrome (EDS) is a disease that attacks the layer hen in production phase and causes the failing of peak production, the decreasing production, and the presence of an egg without a shell. This study was aimed to isolate and identify the EDS virus in chicken layer that was diagnosed as a disease of EDS by hemagglutination (HA) and hemagglutination inhibition (HI) assay. The sample used was chicken�s uterus and residual water of egg washing from FF/Sl eman/2011, along with uterus from specific pathogen free (SPF) layer chicken that was infected by SR/WNO/2011. Specific pathogen free layer chicken which was from Bandung and was passing through the production phase was fed with food which was mixed with egg without shell from SR/WNO/2011. The chicken together with with chicken FF/Sleman/2011 were dissected when symptoms of infection shown and the uterus taken to make it as the sample. Symptoms of infection was shown by the presence of dents or pale eggs. Infundibulum of chicken FF/Sleman/2011 was explored and was found out the egg without eggshell. Then the egg was washed using sterile PBS and became the sample. The three subsequent samples was propagated in allantois fluid of embryonated duck eggs that was 16 days. Allantoic fluid was harvested after incubation for 4 days, then it was tested by with HA and HI assay. HA and HI test with EDS anti serum used chicken�s eritrosit in percentage 0,8. HA test positive result was indicated by erythrocyte cell aggregates formed by the vir al hemagglutinin protein binding to erythrocyte surface receptors. This assay was different with HI assay that uses anti serum Avian Influenza (AI), Newcastle disease (ND), and EDS to test suitability of the virus with that anti serum. The specific anti serum would bind with that virus and block the hemagglutination reaction. HA test in uterus sample of both SR/WNO/2011 and FF/Sleman/2011 showed titer 23 HA units and egg wash water sample of FF/Sleman/2011 showed titer 22 HA units. HI test for comparison with anti serum ND and AI were negative, while tests with anti serum EDS showed positive results. Based on HA and HI test results, we can conclude that the virus which grew in allantoic fluid was EDS virus. [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , FIDYAH FITRAWATI and , Dr. drh. Michael Haryadi Wibowo, M.P. (2013) ISOLASI DAN IDENTIFIKASI VIRUS EGG DROP SYNDROME PADA AYAM LAYER. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=61169 |
| spellingShingle | ETD , FIDYAH FITRAWATI , Dr. drh. Michael Haryadi Wibowo, M.P. ISOLASI DAN IDENTIFIKASI VIRUS EGG DROP SYNDROME PADA AYAM LAYER |
| title | ISOLASI DAN IDENTIFIKASI VIRUS EGG DROP SYNDROME
PADA AYAM LAYER |
| title_full | ISOLASI DAN IDENTIFIKASI VIRUS EGG DROP SYNDROME
PADA AYAM LAYER |
| title_fullStr | ISOLASI DAN IDENTIFIKASI VIRUS EGG DROP SYNDROME
PADA AYAM LAYER |
| title_full_unstemmed | ISOLASI DAN IDENTIFIKASI VIRUS EGG DROP SYNDROME
PADA AYAM LAYER |
| title_short | ISOLASI DAN IDENTIFIKASI VIRUS EGG DROP SYNDROME
PADA AYAM LAYER |
| title_sort | isolasi dan identifikasi virus egg drop syndrome pada ayam layer |
| topic | ETD |
| work_keys_str_mv | AT fidyahfitrawati isolasidanidentifikasiviruseggdropsyndromepadaayamlayer AT drdrhmichaelharyadiwibowomp isolasidanidentifikasiviruseggdropsyndromepadaayamlayer |