| Summary: | The development on goat farm and dairy in Indonesia for the last few
years tend to be increasing, one of them is the Ettawa Crossbreed (PE). The
mastitis case is one of the main issues in maximizing milk production. One of the
bacteria groups causing mastitis is the gram-negative coccobacilli. Treatment
using antibiotics is one of the methods applied in the field. However, antibiotics
with inappropriate dosage and usage can cause bacteria resistance. This research
aims to investigate the sensitivity difference of gram-negative coccobacilli from
PE milk on ampicillin, chloramphenicol, gentamicin, and erythromycin.
This research utilized five types of bacteria isolates which consist of
Escherichia coli, Klebsiella oxytoca, Klebsiella pneumoniae, Citrobacter freundii,
and Serratia marcescens which were isolated from Ettawa Crossbreed milk at
Kalasan PE farm, Yogyakarta. The sensitivity test was conducted through diskdiffusion
method by Kirby-Bauer. The bacteria suspension (the amount of
bacteria is 24x108 CFU/ml) in BHI was eliminated evenly with sterilized cotton
on Muller-Hinton Agar (MHA). Ampicillin, chloramphenicol, gentamicin, and
erythromycin antibiotic disks were placed on the surface of MHA medium and
incubated for 16-18 hours at 35° Celsius. The light zones formed around the
antibiotics disks were then measured and compared with Kirby-Bauer standard
table.
The research result indicates that there are differences in sensitivity on
gram-negative coccobassilli group. Escherichia coli are sensitive to ampicillin,
chloramphenicol, gentamicin, and erythromycin. Klebsiella oxytoca are sensitive
to chloramphenicol, intermediate to gentamicin and resistant to ampicillin and
erythromycin. Klebsiella pneumoniae are sensitive to chloramphenicol, and
resistant to ampicillin, erythromycin, and gentamicin. Citrobacter freundii are
sensitive to ampicillin and chloramphenicol, intermediate to gentamicin and
resistant to erythromycin. Serratia marcescens are sensitive to chloramphenicol,
intermediate sensitive to ampicillin and gentamicin, and resistant to erythromycin.
All gram-negative coccobacilli group bacteria are sensitive to chloramphenicol
and almost all gram-negative coccobacilli group bacteria are resistant to
erythromycin.
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