IDENTIFIKASI ISOLAT Mycobacterium tuberculosis DENGAN KONSENTRASI DNA BERTINGKAT MENGGUNAKAN TEKNIK Polymerase Chain Reaction (PCR)
Mycobacterium tuberculosis is a gram-positive bacteri that causes tuberculosis disease. Tuberculosis is a zoonotic disease and the third cause of death after cardiovascular disease. Method and a proper diagnosis correct and quickly be required. This study aims to diagnose M.tuberculosis at levels co...
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| Summary: | Mycobacterium tuberculosis is a gram-positive bacteri that causes
tuberculosis disease. Tuberculosis is a zoonotic disease and the third cause of
death after cardiovascular disease. Method and a proper diagnosis correct and
quickly be required. This study aims to diagnose M.tuberculosis at levels
concentration using PCR technique for reverens of tuberculosis detection.
Materials used in this study is pure M.tuberculosis DNA from the Center
for Veterinary Research. Initial phase is the DNA isolation. M.tuberculosis
isolates were extracted, and then calculated the concentration of DNA
quantitatively. DNA obtained was diluted to eight different concentrations of the
5000 pg/μl |
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