KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA Tarsius bancanus DAN Tarsius spectrum
Tarsius is an endemic primate in Indonesia but is is getting threatened. Insitu and ex-situ conservation of this primate would yield better results when its genetic information and diversity determined. The main objective of this research is to analyze the genetic diversity in Tarsius sp. based on s...
| Main Authors: | , |
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| Format: | Thesis |
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[Yogyakarta] : Universitas Gadjah Mada
2013
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| _version_ | 1797033530184695808 |
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| author | , HANUNG PERTIWI , Dr. drh. Rini Widayanti, M.P. |
| author_facet | , HANUNG PERTIWI , Dr. drh. Rini Widayanti, M.P. |
| author_sort | , HANUNG PERTIWI |
| collection | UGM |
| description | Tarsius is an endemic primate in Indonesia but is is getting threatened. Insitu
and ex-situ conservation of this primate would yield better results when its
genetic information and diversity determined. The main objective of this research
is to analyze the genetic diversity in Tarsius sp. based on sequences of
NADH dehydrogenase sub-unit 6 (ND6) encoding gene.The DNA samples were
isolated from muscle of T. bancanus and T. spectrum. Samples then amplificated
with Polymerase Chain Reaction (PCR) technique used ND6 Foward and ND6
Reverse primer for T. bancanus samples and ND6 Foward lama and ND6 Reverse
primer for T. spectrum samples. The PCR reaction produced 566 base pair (bp)
(T. bancanus) and 629 bp (T. spectrum) which than sequenced. Sequencing result
then analyzed using MEGA 5.05 version program and compared with some other
Tarsius sp. species obtained from Genbank. Result of this research showed there
were 118 sites of different nucleotides and 40 different amino acid sites. The
average distance of genetic based on nucleotide sequence was 14% and based on
amino acid sequence was 15%. Construction of phylogeny tree based on
nucleotide and amino acid of ND6 gene with Neighbor-Joining method with
bootstrap values 1000 times was able to distinguish between T. bancanus (from
Kalimantan) and T. spectrum (from North Sulawesi). |
| first_indexed | 2024-03-13T22:55:19Z |
| format | Thesis |
| id | oai:generic.eprints.org:121251 |
| institution | Universiti Gadjah Mada |
| last_indexed | 2024-03-13T22:55:19Z |
| publishDate | 2013 |
| publisher | [Yogyakarta] : Universitas Gadjah Mada |
| record_format | dspace |
| spelling | oai:generic.eprints.org:1212512016-03-04T08:38:21Z https://repository.ugm.ac.id/121251/ KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA Tarsius bancanus DAN Tarsius spectrum , HANUNG PERTIWI , Dr. drh. Rini Widayanti, M.P. ETD Tarsius is an endemic primate in Indonesia but is is getting threatened. Insitu and ex-situ conservation of this primate would yield better results when its genetic information and diversity determined. The main objective of this research is to analyze the genetic diversity in Tarsius sp. based on sequences of NADH dehydrogenase sub-unit 6 (ND6) encoding gene.The DNA samples were isolated from muscle of T. bancanus and T. spectrum. Samples then amplificated with Polymerase Chain Reaction (PCR) technique used ND6 Foward and ND6 Reverse primer for T. bancanus samples and ND6 Foward lama and ND6 Reverse primer for T. spectrum samples. The PCR reaction produced 566 base pair (bp) (T. bancanus) and 629 bp (T. spectrum) which than sequenced. Sequencing result then analyzed using MEGA 5.05 version program and compared with some other Tarsius sp. species obtained from Genbank. Result of this research showed there were 118 sites of different nucleotides and 40 different amino acid sites. The average distance of genetic based on nucleotide sequence was 14% and based on amino acid sequence was 15%. Construction of phylogeny tree based on nucleotide and amino acid of ND6 gene with Neighbor-Joining method with bootstrap values 1000 times was able to distinguish between T. bancanus (from Kalimantan) and T. spectrum (from North Sulawesi). [Yogyakarta] : Universitas Gadjah Mada 2013 Thesis NonPeerReviewed , HANUNG PERTIWI and , Dr. drh. Rini Widayanti, M.P. (2013) KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA Tarsius bancanus DAN Tarsius spectrum. UNSPECIFIED thesis, UNSPECIFIED. http://etd.ugm.ac.id/index.php?mod=penelitian_detail&sub=PenelitianDetail&act=view&typ=html&buku_id=61326 |
| spellingShingle | ETD , HANUNG PERTIWI , Dr. drh. Rini Widayanti, M.P. KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA Tarsius bancanus DAN Tarsius spectrum |
| title | KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA Tarsius bancanus DAN Tarsius spectrum |
| title_full | KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA Tarsius bancanus DAN Tarsius spectrum |
| title_fullStr | KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA Tarsius bancanus DAN Tarsius spectrum |
| title_full_unstemmed | KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA Tarsius bancanus DAN Tarsius spectrum |
| title_short | KAJIAN KERAGAMAN GENETIK GEN PENYANDI NADH Dehydrogenase Sub-unit 6 (ND6) PADA Tarsius bancanus DAN Tarsius spectrum |
| title_sort | kajian keragaman genetik gen penyandi nadh dehydrogenase sub unit 6 nd6 pada tarsius bancanus dan tarsius spectrum |
| topic | ETD |
| work_keys_str_mv | AT hanungpertiwi kajiankeragamangenetikgenpenyandinadhdehydrogenasesubunit6nd6padatarsiusbancanusdantarsiusspectrum AT drdrhriniwidayantimp kajiankeragamangenetikgenpenyandinadhdehydrogenasesubunit6nd6padatarsiusbancanusdantarsiusspectrum |